| Gene Symbol |
Rif1
|
| Entrez Gene |
51869
|
| Alt Symbol |
5730435J01Rik, 6530403D07Rik, AU016181, AW549474, D2Ertd145e
|
| Species |
Mouse
|
| Gene Type |
protein-coding
|
| Description |
Rap1 interacting factor 1 homolog (yeast)
|
| Other Description |
mRif1|rap1-interacting factor 1 homolog|telomere-associated protein RIF1
|
| Swissprots |
Q9CS77 Q6T8D9 Q8BTU2 Q7TPD8 Q6PR54 Q8C408
|
| Accessions |
EDL26903 Q6PR54 AK017618 BAB30843 AK018316 BAC25553 AK030007 AK081133 AK083291 BAC38846 AK088688 BAC40506 AK088917 AY428571 AAR87833 AY585206 AAS94124 BC026482 BC055320 AAH55320 CJ101347 JX255737 AFQ31624 XM_006498177 XP_006498240 XM_006498179 XP_006498242 XM_011239135 XP_011237437 XM_011239136 XP_011237438 XM_011239137 XP_011237439 NM_175238 NP_780447
|
| Function |
Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S- phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints (By similarity). {ECO:0000250}.
|
| Subcellular Location |
Nucleus {ECO:0000269|PubMed:15042697}. Chromosome, telomere {ECO:0000269|PubMed:15042697}. Cytoplasm, cytoskeleton, spindle {ECO:0000250}. Note=Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase (By similarity). While the majority of the protein appears nuclear and distinct from normal telomere structures, a small fraction may bind to telomeres in embryonic stem cells. {ECO:0000250}.
|
| Tissue Specificity |
Expressed in Sertoli cells, prospermatagonia, early primary spermatocytes, and in oocytes at all stages of their growth. Expressed in embryonic stem (ES) and embryonic germ (EG) cells: expression is lost upon differentiation. {ECO:0000269|PubMed:15042697}.
|
| Top Pathways |
Signaling pathways regulating pluripotency of stem cells
|
![Western blot using Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kDa corresponding to mouse R(arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubawith the immunizing peptide (data not shown).Approximately 25 µg of MEF whole cell lysawas separated by SDS-PAGE and transfeonto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 µg/ml for 2 h at room temperature followed by washes and reactiowith a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperatuIRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging Systemdeveloped by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://www.bioprodhub.com/system/product_images/ab_products/22/sub_2/21984_0112306.jpeg)
![Western blot using Rif1 polyclonal antibody ( Cat # PAB10275 ) shows detection of a band ~ 265 kDa corresponding to mouse Rif1 ( arrowhead ). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide ( data not shown ). Approximately 25 µg of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 µg/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.](http://www.bioprodhub.com/system/product_images/ab_products/22/sub_2/21985_PAB10275-46-182-1.jpg)
![WB using Anti-Rif1 antibody shows detection of a band ~265 kDa corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). The primary antibody diluted to 1.0ug/ml. IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX was used at 1:10000.](http://www.bioprodhub.com/system/product_images/ab_products/22/sub_4/15687_600-401-871-1-W.jpg)