| Gene Symbol | Rif1 |
|---|---|
| Entrez Gene | 51869 |
| Alt Symbol | 5730435J01Rik, 6530403D07Rik, AU016181, AW549474, D2Ertd145e |
| Species | Mouse |
| Gene Type | protein-coding |
| Description | Rap1 interacting factor 1 homolog (yeast) |
| Other Description | mRif1|rap1-interacting factor 1 homolog|telomere-associated protein RIF1 |
| Swissprots | Q9CS77 Q6T8D9 Q8BTU2 Q7TPD8 Q6PR54 Q8C408 |
| Accessions | EDL26903 Q6PR54 AK017618 BAB30843 AK018316 BAC25553 AK030007 AK081133 AK083291 BAC38846 AK088688 BAC40506 AK088917 AY428571 AAR87833 AY585206 AAS94124 BC026482 BC055320 AAH55320 CJ101347 JX255737 AFQ31624 XM_006498177 XP_006498240 XM_006498179 XP_006498242 XM_011239135 XP_011237437 XM_011239136 XP_011237438 XM_011239137 XP_011237439 NM_175238 NP_780447 |
| Function | Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S- phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints (By similarity). {ECO:0000250}. |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:15042697}. Chromosome, telomere {ECO:0000269|PubMed:15042697}. Cytoplasm, cytoskeleton, spindle {ECO:0000250}. Note=Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase (By similarity). While the majority of the protein appears nuclear and distinct from normal telomere structures, a small fraction may bind to telomeres in embryonic stem cells. {ECO:0000250}. |
| Tissue Specificity | Expressed in Sertoli cells, prospermatagonia, early primary spermatocytes, and in oocytes at all stages of their growth. Expressed in embryonic stem (ES) and embryonic germ (EG) cells: expression is lost upon differentiation. {ECO:0000269|PubMed:15042697}. |
| Top Pathways | Signaling pathways regulating pluripotency of stem cells |
Rif1 CRISPR/Cas9 KO Plasmid (m) - sc-424565 from Santa Cruz Biotechnology
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Rif1 HDR Plasmid (m) - sc-424565-HDR from Santa Cruz Biotechnology
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Rif1 Double Nickase Plasmid (m) - sc-424565-NIC from Santa Cruz Biotechnology
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Rif1 Double Nickase Plasmid (m2) - sc-424565-NIC-2 from Santa Cruz Biotechnology
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Rif1 CRISPR Activation Plasmid (m) - sc-424565-ACT from Santa Cruz Biotechnology
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Rif1 CRISPR Activation Plasmid (m2) - sc-424565-ACT-2 from Santa Cruz Biotechnology
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Rif1 Lentiviral Activation Particles (m) - sc-424565-LAC from Santa Cruz Biotechnology
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Rif1 Lentiviral Activation Particles (m2) - sc-424565-LAC-2 from Santa Cruz Biotechnology
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Rif1 siRNA (m) - sc-62945 from Santa Cruz Biotechnology
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Rif1 shRNA Plasmid (m) - sc-62945-SH from Santa Cruz Biotechnology
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Rif1 shRNA (m) Lentiviral Particles - sc-62945-V from Santa Cruz Biotechnology
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CompoZr® Knockout ZFN Kit - CKOZFN40238 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND40238 from Sigma-Aldrich
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RIF1 siRNA (Mouse) - CRM5218 from Cohesion Biosciences
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RIF1 - MBS827747 from MyBioSource
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shRNA set against Mouse Rif1(NM_175238.5) - SHR182144 from Creative biogene
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RIF1 shRNA Plasmid (Mouse) - CHM5218 from Cohesion Biosciences
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RIF1 miRNA 3'UTR clone - MiUTR1M-10122 from Creative biogene
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