GNAI1

Gene Symbol GNAI1
Entrez Gene 2770
Alt Symbol Gi
Species Human
Gene Type protein-coding
Description guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
Other Description Gi1 protein alpha subunit|adenylate cyclase-inhibiting G alpha protein|guanine nucleotide-binding protein G(i) subunit alpha-1
Swissprots Q9UGA4 C9J3A4 P63096 B4E2V1 A8KA88 P11015 P31871 P04898 Q8TAN5 Q5U074
Accessions AAA35893 AAC03418 EAW77009 EAW77010 EAW77011 EAW77012 P63096 AF055013 AAC09361 AF493905 AAM12619 AI878901 AK292953 BAF85642 AK304442 BAG65263 AL049933 CAB43212 AM393657 CAL38533 BC026326 AAH26326 BT019775 AAV38580 CD299597 DQ892385 ABM83311 DQ895598 ABM86524 DQ895599 ABM86525 M17219 AAA52581 NM_001256414 NP_001243343 NM_002069 NP_002060
Function Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. {ECO:0000269|PubMed:17264214, ECO:0000269|PubMed:17635935}.
Subcellular Location Nucleus {ECO:0000250}. Cytoplasm {ECO:0000269|PubMed:17635935}. Cell membrane {ECO:0000269|PubMed:17635935}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:17635935}. Note=Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane (By similarity). Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody. {ECO:0000250}.
Top Pathways Circadian entrainment, Oxytocin signaling pathway, Estrogen signaling pathway, Adrenergic signaling in cardiomyocytes, Glutamatergic synapse

Gα(i) Antibody - 5290 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various tissues using Gα(i) Antibody.

G α i/o/t/z/gust (H-300) - sc-28586 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
G<sub>α i/o/t/z/gust</sub> (H-300): sc-28586. Immunofluorescence staining of methanol-fixed HeLa cells showing perinuclear and cytoplasmic localization.

G α i-3 (H-7) - sc-365422 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
G<sub>α i-3</sub> (H-7): sc-365422. Western blot analysis of G<sub>α i-3</sub> expression in HeLa whole cell lysate.

G α i-1 (I-20) - sc-391 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
G<sub>α i-1</sub> (I-20): sc-391. Western blot analysis of G<sub>α i-1</sub> expression in bovine brain extract (A) and analysis of rat recombinant G<sub>α i-1</sub>: sc-4232 WB (B).

G α i-1 (SPM397) - sc-56536 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
G<sub>α i-1</sub> (SPM397): sc-56536. Western blot analysis of G<sub>α i-1</sub> expression in SK-N-SH whole cell lysate (A) and rat brain (B) and mouse brain (C) tissue extracts.

G α i-1 (R4) - sc-13533 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
G<sub>αi-1</sub> (R4): sc-13533. Western blot analysis of G<sub>αi-1</sub> expression in rat recombinant G<sub>αi-1</sub>.

G α i-1/2/3 (N-20) - sc-26761 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
G<sub>α i-1/2/3</sub> (N-20): sc-26761. Immunofluorescence staining of methanol-fixed KNRK cells showing membrane localization.

G α i-1/2/3 (35) - sc-136478 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ga i-1/2/3 (35): sc-136478. Western blot analysis of Ga i-1/2/3 expression in rat kidney tissue extract.

G α i-1/2/3 (I-18) - sc-26762 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-G Protein alpha Inhibitor 1+2 antibody - ab3522 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab3522 staining in Human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3522, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-G protein alpha inhibitor 1 antibody [2B8-2A5] - ab118434 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2B8-2A5
ab118434 at 5µg/ml staining G protein alpha inhibitor 1 in Formalin-fixed, Paraffin-embedded Human skin tissue by Immunohistochemistry.

Anti-G protein alpha inhibitor 1 antibody [EPR9441(B)] - ab140125 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9441(B)
Overlay histogram showing SHSY-5Y cells stained with ab140125 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140125, 1/9999 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-G protein alpha inhibitor 1 antibody [EPR9441(B)] (Alexa Fluor® 488) - ab202292 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9441(B)
ab202292 staining G protein alpha inhibitor 1 in SK-N-SH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202292 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in SK-N-SH cells fixed with 100% methanol (5min).

Anti-GNAI1 / Gi Antibody (aa345-354) - LS-C3237 from LifeSpan Bioscience

Prices $265.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GNAI1 / Gi Antibody (aa345-354) - LS-C23812 from LifeSpan Bioscience

Prices $475.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-GNAI1 / Gi Antibody (aa110-123) - LS-C23978 from LifeSpan Bioscience

Prices $445.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal

Anti-GNAI1 / Gi Antibody - LS-C23979 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-GNAI1 / Gi Antibody - LS-C26697 from LifeSpan Bioscience

Prices $560.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-GNAI1 / Gi Antibody (Azide-free) - LS-C36538 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-GNAI1 / Gi Antibody (clone R4.5) - LS-C87972 from LifeSpan Bioscience

Prices $280.00, $530.00, $810.00
Sizes 20 µg, 100 µg, 1 ml
Host Mouse
Clonality Monoclonal
Clone R4.5

Anti-GNAI1 / Gi Antibody (clone R4.5, Azide-free) - LS-C87973 from LifeSpan Bioscience

Prices $595.00, $880.00
Sizes 100 µg, 200 µg
Host Mouse
Clonality Monoclonal
Clone R4.5

Anti-GNAI1 / Gi Antibody - LS-C314087 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GNAI1 / Gi Antibody (N-Terminus) - LS-C203274 from LifeSpan Bioscience

Prices $245.00
Sizes 40 µg
Host Rabbit
Clonality Polyclonal

Anti-GNAI1 / Gi Antibody (aa146-195) - LS-C81891 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-GNAI1 / Gi Antibody (clone R4.5) IHC-plus™ - LS-B2546 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone R4.5
Anti-GNAI1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2546 dilution 1:50.
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