IFT20

Gene Symbol IFT20
Entrez Gene 90410
Alt Symbol -
Species Human
Gene Type protein-coding
Description intraflagellar transport 20
Other Description intraflagellar transport 20 homolog|intraflagellar transport protein 20 homolog|intraflagellar transport protein IFT20
Swissprots Q9BUG5 J3QL09 Q5GLZ2 Q8IY31
Accessions EAW51070 EAW51071 EAW51072 EAW51073 EAW51074 EAW51075 EAW51076 Q8IY31 AA490722 AF070643 AI768816 AK297769 BAH12662 AK309232 AL713094 AY224601 AAP50265 BC002640 AAH02640 BC038094 AAH38094 BE619475 BQ639919 DQ599851 HQ448333 ADQ32813 JF432488 ADZ15705 NM_001267774 NP_001254703 NM_001267775 NP_001254704 NM_001267776 NP_001254705 NM_001267777 NP_001254706 NM_001267778 NP_001254707 NM_174887 NP_777547
Function Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment. {ECO:0000269|PubMed:16775004}.
Subcellular Location Golgi apparatus, cis-Golgi network {ECO:0000250}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250}. Note=Present at the centrosomes during the cell cycle and associated with the proximal portion of the mother centriole and the lateral aspect of the daughter centriole. Associated with basal body at the base of primary cilia (By similarity). {ECO:0000250}.
Tissue Specificity Expressed in almost all tissues. {ECO:0000269|PubMed:14672413}.

IFT20 (E-13) - sc-107626 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

IFT20 (N-12) - sc-107627 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-IFT20 antibody - ab103693 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ab103693 stained WI 38 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103693 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-IFT20 antibody - ab73110 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-IFT20 antibody (ab73110) at 1/500 dilutionLane 1 : IFT20 transfected 293T cell lineLane 2 : Non transfected 293T cell lineLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutionObserved band size : 16 kDa (why is the actual band size different from the predicted?)

Anti-IFT20 Antibody (aa44-71, AP) - LS-C208942 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IFT20 Antibody (aa44-71, Biotin) - LS-C208945 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IFT20 Antibody (aa44-71, APC) - LS-C208944 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IFT20 Antibody (aa44-71, Azide-free, HRP) - LS-C208947 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IFT20 Antibody (aa44-71, FITC) - LS-C208946 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IFT20 Antibody (aa44-71, PE) - LS-C208948 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IFT20 Antibody (aa50-80) - LS-C322160 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IFT20 Antibody - LS-C308413 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-IFT20 Antibody (clone 3F3) - LS-C139316 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F3
Western blot of IFT20 expression in transfected 293T cell line by IFT20 monoclonal antibody (M02), clone 3F3.

Anti-IFT20 Antibody (aa44-71) - LS-C165135 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
IFT20 Antibody IHC of formalin-fixed and paraffin-embedded hepatocarcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

IFT20 Antibody - NBP1-86424 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

IFT20 Antibody - H00090410-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

IFT20 Antibody (3F3) - H00090410-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F3
Western Blot: IFT20 Antibody (3F3) [H00090410-M02] - Analysis of IFT20 expression in transfected 293T cell line by IFT20 monoclonal antibody (M02), clone 3F3.Lane 1: IFT20 transfected lysate(16 KDa).Lane 2: Non-transfected lysate.

IFT20 Polyclonal Antibody - bs-15561R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-IFT20 antibody produced in mouse - SAB1408170 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-IFT20: <B>Cat. No. SAB1408170</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~16.3 kDa using IFT20 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Monoclonal Anti-IFT20 antibody produced in mouse - SAB1403414 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F3
<B>Immunofluorescence</B><BR/>Monoclonal Anti-IFT20: <B>Cat. No. SAB1403414</B>: Antibody concentration: 10 μg/mL using HeLa cell.

Anti-IFT20 antibody produced in rabbit - HPA021376 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-IFT20: <B>Cat. No. HPA021376</B>: Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in lymphoid cells.

IFT20 Antibody - middle region (OAAB02550) - OAAB02550 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

IFT20-Antibody-Center - AP5133c from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of IFT20 Antibody (Center) (Cat. #AP5133c) in mouse lung tissue lysates (35ug/lane).IFT20 (arrow) was detected using the purified Pab.

IFT20 antibody - GTX117031 from GeneTex

Prices $119.00
Sizes 25 μl
Host Rabbit
Clonality Polyclonal
Anti-IFT20 antibody used in ICC/IF. GTX117031

Anti -IFT20 (Intraflagellar Transport Protein 20 Homolog) - 128322 from USBiological Life Science

Host Mouse
Clonality Polyclonal
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