HMGCL

Gene Symbol	HMGCL
Entrez Gene	3155
Alt Symbol	HL
Species	Human
Gene Type	protein-coding
Description	3-hydroxymethyl-3-methylglutaryl-CoA lyase
Other Description	3-hydroxy-3-methylglutarate-CoA lyase\|3-hydroxy-3-methylglutaryl-CoA lyase\|3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase\|HMG-CoA lyase\|hydroxymethylglutaricaciduria\|hydroxymethylglutaryl-CoA lyase, mitochondrial\|mitochondrial 3-hydroxy-3-methylglutaryl-CoA lyase
Swissprots	Q96FP8 B7UCC6 D3Y5K7 B4DUP4 P35914 Q6IBC0
Accessions	AER36554 EAW95094 EAW95095 EAW95096 EAW95097 P35914 AI202943 AK122801 BAG53736 AK300733 BAG62406 AK309129 AK313869 BAG36597 BC010570 AAH10570 BC021145 BM981610 BT009792 AAP88794 CA306929 CR456884 CAG33165 DA425822 DQ892083 ABM83009 DQ895276 ABM86202 FJ472654 ACK58684 GU433941 ADD21697 L07033 AAA92733 NM_000191 NP_000182 NM_001166059 NP_001159531
Function	Key enzyme in ketogenesis (ketone body formation). Terminal step in leucine catabolism. Ketone bodies (beta- hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism. {ECO:0000269\|PubMed:22847177, ECO:0000269\|PubMed:22865860, ECO:0000269\|PubMed:8566388}.
Subcellular Location	Mitochondrion matrix. Peroxisome. Note=Unprocessed form is peroxisomal.
Tissue Specificity	Highest expression in liver. Expressed in pancreas, kidey, intestine, testis, fibroblasts and lymphoblasts. Very low expression in brain and skeletal muscle. The relative expression of isoform 2 (at mRNA level) is highest in heart (30%), skeletal muscle (22%), and brain (14%). {ECO:0000269\|PubMed:21952825}.
Top Pathways	Peroxisome, Synthesis and degradation of ketone bodies, Valine, leucine and isoleucine degradation, Butanoate metabolism

HMGCL/HMGCLL1 (H-50) - sc-292720 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

HMGCL (T-20) - sc-101973 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

HMGCL (T-20): sc-101973. Western blot analysis of HMGCL expression in fetal liver tissue extract.

HMGCL (63Z) - sc-100548 from Santa Cruz Biotechnology

Prices	$295.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

HMGCL (63Z): sc-100548. Western blot analysis of HMGCL expression in non-transfected: sc-110760 (A) and human HMGCL transfected: sc-111008 (B) 293 whole cell lysates.

HMGCL (G-12) - sc-131552 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

HMGCL (G-12): sc-131552. Western blot analysis of HMGCL expression in mouse liver tissue extract.

HMGCL (Y-21) - sc-133661 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	50 µg/0.5 ml
Host	Rabbit
Clonality	Polyclonal

HMGCL (Y-21): sc-133661. Western blot analysis of HMGCL expression in A-431 whole cell lysate.

Anti-HMGCL antibody - ab104500 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Cytoplasmic staining of HMGCL in formalin-fixed, paraffin-embedded Human fetal lymph node tissue using 1/100 ab104500.

Anti-HMGCL antibody - ab55173 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-HMGCL antibody [9F5AB9] - ab110295 from Abcam

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	9F5AB9

Immunocytochemistry image of ab110295 stained Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with ab110295 at 5 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. Target protein locates mainly in mitochondria.

Anti-HMGCL antibody [EPR15550] - N-terminal - ab187648 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR15550

Flow cytometric analysis of 2% paraformaldehyde fixed 293 cells labeling HMGCL with ab187648 at 1/20 (red) or Rabbit monoclonal IgG (green) followed by Goat anti rabbit IgG (FITC) antibody at 1/150.

Anti-HMGCL antibody [EPR15551] - ab197022 from Abcam

Prices	$347.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR15551

Anti-HMGCL antibody [EPR15551] (ab197022) at 1/1000 dilution + 293 (Human epithelial cells from embryonic kidney) cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Anti-HMGCL Antibody - LS-C178400 from LifeSpan Bioscience

Prices	$255.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-HMGCL Antibody (aa1-326, clone 10D211, Azide-free) - LS-C183702 from LifeSpan Bioscience

Host	Mouse
Clonality	Monoclonal
Clone	10D211

Anti-HMGCL Antibody (aa81-130) - LS-C40341 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-HMGCL Antibody (C-Terminus) - LS-C40342 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-HMGCL Antibody - LS-C310625 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HMGCL Antibody (aa160-190) - LS-C322009 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HMGCL Antibody - LS-C310628 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-HMGCL Antibody (aa70-99) - LS-C322010 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HMGCL Antibody (aa1-325) - LS-C186043 from LifeSpan Bioscience

Prices	$335.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

IHC of paraffin-embedded SW480 xenograft using HMGCL antibody at 1:500 dilution.

Anti-HMGCL Antibody (aa160-190, AP) - LS-C258637 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HMGCL Antibody (aa160-190, APC) - LS-C258638 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HMGCL Antibody (aa160-190, Biotin) - LS-C258639 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HMGCL Antibody (aa251-300) - LS-C170247 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-HMGCL Antibody (aa161-190) - LS-C164935 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

HMGCL Antibody western blot of WiDr cell line lysates (35 ug/lane). The HMGCL antibody detected the HMGCL protein (arrow).

Anti-HMGCL Antibody (aa71-99) - LS-C159851 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

HMGCL Antibody western blot of K562 cell line lysates (35 ug/lane). The HMGCL antibody detected the HMGCL protein (arrow).

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