RRN3

Gene Symbol	RRN3
Entrez Gene	54700
Alt Symbol	A-270G1.2, TIFIA
Species	Human
Gene Type	protein-coding
Description	RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)
Other Description	RNA polymerase I-specific transcription initiation factor RRN3\|TIF-IA\|transcription initiation factor IA\|transcription initiation factor TIF-IA
Swissprots	Q3MHU9 A2RTY9 B4E3T2 B4E0J7 Q9NYV6 Q6IPL4 Q9H4F0
Accessions	EAW54754 Q9NYV6 AF227156 AAF66160 AJ272050 CAC07955 AK225662 AK295486 BAG58409 AK300253 BAG62018 AK302994 BAG64131 AK303405 BAG64459 AK304856 BAG65594 AK314769 BAG37307 AK316520 BAH14891 AL110238 BC036182 BC071868 AAH71868 BC104660 AAI04661 BC132688 AAI32689 BC132690 AAI32691 BU676519 CK906270 DA764092 DC376629 HQ258337 ADR83091 XM_005255376 XP_005255433 XM_005255377 XP_005255434 XM_006725198 XP_006725261 XM_006725199 XP_006725262 XM_011522540 XP_011520842 XM_011522541 XP_011520843 XM_011522542 XP_011520844 XM_011546705 XP_011545007 XM_011546706 XP_011545008 NM_001301064 NP_001287993 NM_018427 NP_060897
Function	Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction (By similarity). {ECO:0000250}.
Subcellular Location	Nucleus, nucleolus {ECO:0000269\|PubMed:11250903}.

Rrn3 (D-9) - sc-390464 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

Rrn3 (D-9): sc-390464. Western blot analysis of Rrn3 expression in Jurkat (A) and HeLa (B) whole cell lysates.

Rrn3 (E-20) - sc-11804 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Rrn3 (E-20): sc-11804. Western blot analysis of Rrn3 expression in Jurkat (A), HeLa (B) and K-562 (C) whole cell lysates.

Rrn3 (C-20) - sc-11805 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Rrn3 (C-20): sc-11805. Western blot analysis of Rrn3 expression in HeLa (A), Jurkat (B) and K-562 (C) whole cell lysates.

Rrn3 (H-300) - sc-366331 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Rrn3 (Y-23) - sc-133978 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	50 µg/0.5 ml
Host	Rabbit
Clonality	Polyclonal

Rrn3 (Y-23): sc-133978. Western blot analysis of Rrn3 expression in Jurkat whole cell lysate.

Anti-RRN3 (phospho S649) antibody - ab138651 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-RRN3 (phospho S649) antibody (ab138651) at 1/500 dilution + 24 hour starved Jurkat cell extract at 30 µg

Anti-RRN3 antibody - ab169511 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-RRN3 antibody (ab169511) at 1 µg/mlLane 1 : RRN3 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-RRN3 antibody - ab112052 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-RRN3 antibody (ab112052) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-RRN3 antibody - ab79375 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-RRN3 antibody (ab79375) at 1/500 dilutionLane 1 : HeLa cell extracts, treated with calyculin A (50ng/ml, 30mins)Lane 2 : HeLa cell extracts, treated with calyculin A (50ng/ml, 30mins) with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-RRN3 antibody - ab42539 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RRN3 antibody (ab42539) at 0.5 µg/ml + Jurkat cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-TIF-IA / RRN3 Antibody (aa575-624) - LS-C110373 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

TIF-IA / RRN3 antibody Western Blot of HeLa cell lysate. RRN3 is supported by BioGPS gene expression data to be expressed in HeLa.

Anti-TIF-IA / RRN3 Antibody (aa331-380) - LS-C31361 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-TIF-IA / RRN3 Antibody (Val643) - LS-C193122 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-TIF-IA / RRN3 Antibody (C-Terminus) - LS-C30139 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-TIF-IA / RRN3 Antibody (aa277-305, AP) - LS-C223949 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-TIF-IA / RRN3 Antibody (aa277-305, APC) - LS-C223950 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-TIF-IA / RRN3 Antibody (aa277-305, FITC) - LS-C223952 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-TIF-IA / RRN3 Antibody (aa277-305, Biotin) - LS-C223951 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-TIF-IA / RRN3 Antibody (aa277-305, Azide-free, HRP) - LS-C223953 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-TIF-IA / RRN3 Antibody (aa277-305, PE) - LS-C223954 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-TIF-IA / RRN3 Antibody (aa602-651) - LS-C118376 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of extracts from HeLa cells, treated with calyculin A 50 ng/ml 30', using TIF-IA (Ab-649) Antibody. The lane on the right is treated with the synthesized peptide.

Anti-TIF-IA / RRN3 Antibody (phospho-Ser649) - LS-C291645 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-TIF-IA / RRN3 Antibody (aa276-305) - LS-C324840 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-TIF-IA / RRN3 Antibody (aa277-305) - LS-C158901 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

RRN3 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The RRN3 antibody detected the RRN3 protein (arrow).

Anti-TIF-IA / RRN3 Antibody (phospho-Ser649) - LS-C199654 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IHC of paraffin-embedded human brain, using TIF-IA (Phospho-Ser649) Antibody. The sample on the right was incubated with synthetic peptide.

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