Gene Symbol | IWS1 |
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Entrez Gene | 55677 |
Alt Symbol | - |
Species | Human |
Gene Type | protein-coding |
Description | IWS1 homolog (S. cerevisiae) |
Other Description | IWS1-like protein|interacts with Spt6|protein IWS1 homolog |
Swissprots | Q9NV97 Q96MI7 Q6P157 Q8N3E8 Q96ST2 Q9NWH8 Q2TB65 |
Accessions | EAW95321 Q96ST2 AI638093 AK000868 BAA91402 AK001717 BAA91858 AK027561 BAB55198 AK056881 BAB71301 AK294669 BAG57836 AK295970 BAG58746 AK296848 BAG59414 AK309006 AL834178 CAD38875 BC017012 AAH17012 BC022051 BC022892 BC065279 AAH65279 BC110536 AAI10537 BC110537 AAI10538 DA268882 XM_005263707 XP_005263764 XM_006712626 XP_006712689 XM_011511450 XP_011509752 XM_011511451 XP_011509753 XM_011511452 XP_011509754 XR_427099 XR_922960 NM_017969 NP_060439 |
Function | Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. {ECO:0000269|PubMed:17184735, ECO:0000269|PubMed:17234882, ECO:0000269|PubMed:19141475}. |
Subcellular Location | Nucleus {ECO:0000255|PROSITE- ProRule:PRU00649, ECO:0000269|PubMed:17184735, ECO:0000269|PubMed:17234882}. |
hIws1 CRISPR/Cas9 KO Plasmid (h) - sc-407220 from Santa Cruz Biotechnology
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hIws1 HDR Plasmid (h) - sc-407220-HDR from Santa Cruz Biotechnology
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hIws1 Double Nickase Plasmid (h) - sc-407220-NIC from Santa Cruz Biotechnology
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hIws1 Double Nickase Plasmid (h2) - sc-407220-NIC-2 from Santa Cruz Biotechnology
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hIws1 CRISPR Activation Plasmid (h) - sc-407220-ACT from Santa Cruz Biotechnology
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hIws1 CRISPR Activation Plasmid (h2) - sc-407220-ACT-2 from Santa Cruz Biotechnology
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hIws1 Lentiviral Activation Particles (h) - sc-407220-LAC from Santa Cruz Biotechnology
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hIws1 Lentiviral Activation Particles (h2) - sc-407220-LAC-2 from Santa Cruz Biotechnology
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hIws1 siRNA (h) - sc-94726 from Santa Cruz Biotechnology
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hIws1 shRNA Plasmid (h) - sc-94726-SH from Santa Cruz Biotechnology
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hIws1 shRNA (h) Lentiviral Particles - sc-94726-V from Santa Cruz Biotechnology
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CompoZr® Knockout ZFN Kit - CKOZFN10848 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND10848 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR01509 from Sigma-Aldrich
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IWS1 siRNA (Human) - CRJ0826 from Cohesion Biosciences
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IWS1, siRNA - MBS8201629 from MyBioSource
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shRNA set against Human IWS1 (NM_017969.2) - SHH321811 from Creative biogene
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Human IWS1 siRNA - orb271327 from Biorbyt
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IWS1 shRNA Plasmid (Human) - CHJ0826 from Cohesion Biosciences
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IWS1 miRNA 3'UTR clone - MiUTR3H-15159 from Creative biogene
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