Gene Symbol | CNOT1 |
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Entrez Gene | 23019 |
Alt Symbol | AD-005, CDC39, NOT1, NOT1H |
Species | Human |
Gene Type | protein-coding |
Description | CCR4-NOT transcription complex, subunit 1 |
Other Description | CCR4-NOT transcription complex subunit 1|CCR4-associated factor 1|NOT1 (negative regulator of transcription 1, yeast) homolog|adrenal gland protein AD-005|negative regulator of transcription subunit 1 homolog |
Swissprots | Q8IWB8 Q9UFR8 Q9BVZ6 Q9Y2L0 Q9UI27 Q8TB53 Q7Z3K2 Q68DX7 A5YKK6 |
Accessions | A5YKK6 AB023224 BAA76851 AF110778 AAF14861 AJ346264 AK056927 BAG51828 AK075125 AK092885 BAG52622 AK300281 BAG62040 AK301092 BAH13408 AK302495 BAG63779 AL117492 CAB55960 AL832769 AL833549 BC000779 AAH00779 BC011861 BC024317 AAH24317 BC027969 BC040523 AAH40523 BM023419 BM968443 BX537840 CAD97851 BX647815 CB162525 CR749237 CAH18093 DB456343 DQ579255 EF553522 ABQ66268 NM_001265612 NP_001252541 NM_016284 NP_057368 NM_206999 NP_996882 NR_049763 |
Function | Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA- mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of emryonic stem (ES) cell identity. {ECO:0000269|PubM |
Subcellular Location | Cytoplasm, P-body {ECO:0000269|PubMed:21976065}. Nucleus {ECO:0000305|PubMed:21976065}. Note=NANOS2 promotes its localization to P-body. {ECO:0000250}. |
Tissue Specificity | Strongly expressed in brain, heart, thymus, spleen, kidney, liver, placenta and lung. Weakly expressed in skeletal muscle and colon. {ECO:0000269|PubMed:10637334}. |
Top Pathways | RNA degradation |
CNOT1 CRISPR/Cas9 KO Plasmid (h) - sc-403431 from Santa Cruz Biotechnology
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CNOT1 HDR Plasmid (h) - sc-403431-HDR from Santa Cruz Biotechnology
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CNOT1 Double Nickase Plasmid (h) - sc-403431-NIC from Santa Cruz Biotechnology
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CNOT1 Double Nickase Plasmid (h2) - sc-403431-NIC-2 from Santa Cruz Biotechnology
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CNOT1 CRISPR Activation Plasmid (h) - sc-403431-ACT from Santa Cruz Biotechnology
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CNOT1 CRISPR Activation Plasmid (h2) - sc-403431-ACT-2 from Santa Cruz Biotechnology
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CNOT1 Lentiviral Activation Particles (h) - sc-403431-LAC from Santa Cruz Biotechnology
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CNOT1 Lentiviral Activation Particles (h2) - sc-403431-LAC-2 from Santa Cruz Biotechnology
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CNOT1 siRNA (h) - sc-93370 from Santa Cruz Biotechnology
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CNOT1 shRNA Plasmid (h) - sc-93370-SH from Santa Cruz Biotechnology
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CNOT1 shRNA (h) Lentiviral Particles - sc-93370-V from Santa Cruz Biotechnology
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CNOT1 RNAi - H00023019-R01 from Novus Biologicals
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CNOT1 RNAi - H00023019-R02 from Novus Biologicals
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MISSION® esiRNA - EHU072681 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN6318 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND6318 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR07580 from Sigma-Aldrich
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CNOT1 siRNA (Human) - CRH7934 from Cohesion Biosciences
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CNOT1 - MBS8223424 from MyBioSource
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shRNA set against Human CNOT1 (NM_016284.4) - SHH266404 from Creative biogene
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Human CNOT1 siRNA - orb268436 from Biorbyt
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CNOT1 shRNA Plasmid (Human) - CHH7934 from Cohesion Biosciences
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CNOT1 miRNA 3'UTR clone - MiUTR3H-07205 from Creative biogene
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CNOT1 miRNA 3'UTR clone - MiUTR3H-07206 from Creative biogene
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