BHMT

Gene Symbol BHMT
Entrez Gene 635
Alt Symbol BHMT1, HEL-S-61p
Species Human
Gene Type protein-coding
Description betaine--homocysteine S-methyltransferase
Other Description betaine--homocysteine S-methyltransferase 1|epididymis secretory sperm binding protein Li 61p
Swissprots Q93088 Q9UNI9
Accessions AAD22043 AAQ90058 EAW95831 EAW95832 Q93088 AK298308 BAG60562 AK303780 BAG64739 AK315224 BAG37653 BC012616 AAH12616 BM678994 DA370221 EU794592 ACJ13646 U50929 AAC50668 NM_001713 NP_001704
Function Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Subcellular Location Cytoplasm.
Tissue Specificity Found exclusively in liver and kidney. {ECO:0000269|PubMed:9281325}.
Top Pathways Glycine, serine and threonine metabolism, Cysteine and methionine metabolism

BHMT (H-7) - sc-390299 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BHMT (H-7): sc-390299. Western blot analysis of BHMT expression in human kidney tissue extract.

BHMT (E-15) - sc-162568 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BHMT (E-15): sc-162568. Western blot analysis of BHMT expression in mouse kidney tissue extract.

BHMT (H-46) - sc-134648 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BHMT (H-46): sc-134648. Western blot analysis of BHMT expression in mouse kidney tissue extract.

BHMT (O-24) - sc-130977 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
BHMT (O-24): sc-130977. Western blot analysis of BHMT expression in non-transfected: sc-117752 (A) and mouse BHMT transfected: sc-118805 (B) 293T whole cell lysates.

BHMT (3D6) - sc-69708 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
BHMT (3D6): sc-69708. Western blot analysis of BHMT expression in human kidney tissue extract.

Anti-BHMT antibody - ab180796 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-BHMT antibody (ab180796) at 1/500 dilution + 293T cell lysate

Anti-BHMT antibody [clone 3D6] - ab52144 from Abcam

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone clone 3D6
Overlay histogram showing HepG2 cells stained with ab52144 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52144, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-BHMT antibody [EP6781] - ab137095 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP6781
All lanes : Anti-BHMT antibody [EP6781] (ab137095) at 1/1000 dilutionLane 1 : Human fetal liver lysateLane 2 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-BHMT antibody [EPR6782] - ab124992 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6782
All lanes : Anti-BHMT antibody [EPR6782] (ab124992) at 1/1000 dilutionLane 1 : Human fetal liver lysateLane 2 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-BHMT Antibody (aa1-406) - LS-C35138 from LifeSpan Bioscience

Prices $355.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal

Anti-BHMT Antibody (aa49-98) - LS-C80427 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
BHMT antibody LS-C80427 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.

Anti-BHMT Antibody (aa327-376) - LS-C80428 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
BHMT antibody LS-C80428 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.

Anti-BHMT Antibody (aa1-406, clone 3D6) - LS-C93707 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3D6
Tissue lysates of mouse brain (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human APP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-BHMT Antibody (clone 3E11) IHC-plus™ - LS-B10964 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 3E11
Anti-BHMT antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10964 concentration 10 ug/ml.

Anti-BHMT Antibody - LS-C306543 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-BHMT Antibody - LS-C192478 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of BHMT pAb in extracts from 293T cells.

Anti-BHMT Antibody (C-Terminus, clone EPR6782) - LS-C138969 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6782
IHC of paraffin-embedded human liver tissue using anti-BHMT antibody.

Anti-BHMT Antibody (clone 10B3) - LS-C115014 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 10B3
Anti-BHMT mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BHMT.

Anti-BHMT Antibody - LS-C331359 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of Recombinant protein cell lines, using BHMT antibody.

Anti-BHMT Antibody (clone 2F6) - LS-C115092 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2F6
IHC of paraffin-embedded Human liver tissue using anti-BHMT mouse monoclonal antibody.

Anti-BHMT Antibody (aa199-406) - LS-C185513 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded SG xenograft using BHMT antibody at 1:100 dilution.

Anti-BHMT Antibody (aa1-243) - LS-C185517 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
BHMT antibody detects BHMT protein at cytosol on Colon Carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded Colon Carcinoma. BHMT antibody dilution:1:500.

Anti-BHMT Antibody (aa391-402) IHC-plus™ - LS-B3257 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-BHMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3257 concentration 5 ug/ml.

Anti-BHMT Antibody (clone 8C11H5) IHC-plus™ - LS-B4100 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 8C11H5
Anti-BHMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4100 dilution 1:200.

BHMT Antibody (3E11) - NBP2-02349 from Novus Biologicals

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E11
Western Blot: BHMT Antibody (3E11) [NBP2-02349] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.
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