| Gene Symbol | OTUB1 |
|---|---|
| Entrez Gene | 55611 |
| Alt Symbol | HSPC263, OTB1, OTU1 |
| Species | Human |
| Gene Type | protein-coding |
| Description | OTU deubiquitinase, ubiquitin aldehyde binding 1 |
| Other Description | OTU domain, ubiquitin aldehyde binding 1|OTU domain-containing ubiquitin aldehyde-binding protein 1|OTU-domain Ubal-binding 1|deubiquitinating enzyme OTUB1|otubain-1|ubiquitin thioesterase OTUB1|ubiquitin-specific protease otubain 1|ubiquitin-specific-processing protease OTUB1 |
| Swissprots | Q9P0B8 Q32Q78 Q96FW1 Q9NXQ4 Q96II3 |
| Accessions | CAH05305 EAW74187 EAW74188 EAW74189 EAW74190 EAW74191 Q96FW1 AF161381 AAF28941 AK000120 BAA90956 AK091830 AK098029 BAG53567 AK225133 AK298287 BAG60547 AK303228 BAG64315 AK307413 AL096714 CAH56412 AY177200 AAO27702 BC007519 AAH07519 BC010368 AAH10368 BC107701 AAI07702 CK906312 NM_017670 NP_060140 NR_003089 |
| Function | Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys- 48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin. |
| Subcellular Location | Cytoplasm {ECO:0000250}. |
| Tissue Specificity | Isoform 1 is ubiquitous. Isoform 2 is expressed only in lymphoid tissues such as tonsils, lymph nodes and spleen, as well as peripheral blood mononuclear cells. {ECO:0000269|PubMed:12704427, ECO:0000269|PubMed:14661020}. |
OTUB1 CRISPR/Cas9 KO Plasmid (h) - sc-407665 from Santa Cruz Biotechnology
|
||||||||
OTUB1 HDR Plasmid (h) - sc-407665-HDR from Santa Cruz Biotechnology
|
||||||||
OTUB1 Double Nickase Plasmid (h) - sc-407665-NIC from Santa Cruz Biotechnology
|
||||||||
OTUB1 Double Nickase Plasmid (h2) - sc-407665-NIC-2 from Santa Cruz Biotechnology
|
||||||||
OTUB1 CRISPR Activation Plasmid (h) - sc-407665-ACT from Santa Cruz Biotechnology
|
||||||||
OTUB1 CRISPR Activation Plasmid (h2) - sc-407665-ACT-2 from Santa Cruz Biotechnology
|
||||||||
OTUB1 Lentiviral Activation Particles (h) - sc-407665-LAC from Santa Cruz Biotechnology
|
||||||||
OTUB1 Lentiviral Activation Particles (h2) - sc-407665-LAC-2 from Santa Cruz Biotechnology
|
||||||||
OTUB1 siRNA (h) - sc-76014 from Santa Cruz Biotechnology
|
||||||||
OTUB1 shRNA Plasmid (h) - sc-76014-SH from Santa Cruz Biotechnology
|
||||||||
OTUB1 shRNA (h) Lentiviral Particles - sc-76014-V from Santa Cruz Biotechnology
|
||||||||
MISSION® esiRNA - EHU041621 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit - CKOZFN16194 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND16194 from Sigma-Aldrich
|
||||||||
OTUB1 siRNA (Human) - CRJ0771 from Cohesion Biosciences
|
||||||||
Human OTUB1 siRNA - orb271272 from Biorbyt
|
||||||||
OTUB1 shRNA Plasmid (Human) - CHJ0771 from Cohesion Biosciences
|