PRKDC
| Gene Symbol | PRKDC |
|---|---|
| Entrez Gene | 5591 |
| Alt Symbol | DNA-PKcs, DNAPK, DNPK1, HYRC, HYRC1, IMD26, XRCC7, p350 |
| Species | Human |
| Gene Type | protein-coding |
| Description | protein kinase, DNA-activated, catalytic polypeptide |
| Other Description | DNA-PK catalytic subunit|DNA-dependent protein kinase catalytic subunit|hyper-radiosensitivity of murine scid mutation, complementing 1|p460 |
| Swissprots | Q9UME3 Q7Z611 Q13327 Q59H99 Q96SE6 P78528 Q14175 P78527 Q13337 |
| Accessions | AAA79244 AAB51722 AAC52019 AAK40350 AAP69525 BAB79635 EAW86680 EAW86681 EAW86682 EAW86683 P78527 AB208860 BAD92097 AK296833 BAG59403 BC017494 BC024963 BC037968 U34994 AAC50210 U35835 AAA79184 U47077 AAB39925 XM_011517567 XP_011515869 XM_011517568 XP_011515870 NM_001081640 NP_001075109 NM_006904 NP_008835 |
| Function | Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylat |
| Subcellular Location | Nucleus. Nucleus, nucleolus. |
| Top Pathways | Cell cycle, Non-homologous end-joining |
DNA-PK CS CRISPR/Cas9 KO Plasmid (h) - sc-400337 from Santa Cruz Biotechnology
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DNA-PK CS HDR Plasmid (h) - sc-400337-HDR from Santa Cruz Biotechnology
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DNA-PK CS Double Nickase Plasmid (h) - sc-400337-NIC from Santa Cruz Biotechnology
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DNA-PK CS Double Nickase Plasmid (h2) - sc-400337-NIC-2 from Santa Cruz Biotechnology
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DNA-PK CS CRISPR Activation Plasmid (h) - sc-400337-ACT from Santa Cruz Biotechnology
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DNA-PK CS CRISPR Activation Plasmid (h2) - sc-400337-ACT-2 from Santa Cruz Biotechnology
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DNA-PK CS Lentiviral Activation Particles (h) - sc-400337-LAC from Santa Cruz Biotechnology
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DNA-PK CS Lentiviral Activation Particles (h2) - sc-400337-LAC-2 from Santa Cruz Biotechnology
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DNA-PK CS siRNA (h) - sc-35200 from Santa Cruz Biotechnology
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DNA-PK CS shRNA Plasmid (h) - sc-35200-SH from Santa Cruz Biotechnology
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DNA-PK CS shRNA (h) Lentiviral Particles - sc-35200-V from Santa Cruz Biotechnology
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DNA-PKcs RNAi - H00005591-R03 from Novus Biologicals
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MISSION® esiRNA - EHU123791 from Sigma-Aldrich
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MISSION® siRNA - SIHK1860 from Sigma-Aldrich
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MISSION® siRNA - SIHK1859 from Sigma-Aldrich
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MISSION® siRNA - SIHK1858 from Sigma-Aldrich
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MISSION® siRNA - SIHK1857 from Sigma-Aldrich
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MISSION® siRNA - SIHK1861 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17426 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN1596 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1596 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17426 from Sigma-Aldrich
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PRKDC siRNA (Human) - CRH3761 from Cohesion Biosciences
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shRNA set against Human PRKDC(NM_006904.6) - SHR140764 from Creative biogene
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shRNA set against Human PRKDC(NM_001081640.1) - SHR140808 from Creative biogene
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