Gene Symbol | TLK1 |
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Entrez Gene | 9874 |
Alt Symbol | PKU-beta |
Species | Human |
Gene Type | protein-coding |
Description | tousled-like kinase 1 |
Other Description | SNARE protein kinase SNAK|serine threonine protein kinase|serine/threonine-protein kinase tousled-like 1 |
Swissprots | Q14150 Q8N591 Q9Y4F6 Q9NYH2 B4DX87 B3KR15 Q9UKI8 |
Accessions | AAX93162 AAY15077 EAX11220 EAX11221 EAX11222 EAX11223 Q9UKI8 AB004885 BAA20562 AF162666 AAF03094 AF246219 AAF71263 AK090779 BAG52227 AK091975 BAG52453 AK292508 BAF85197 AK301857 BAG63299 BC032657 AAH32657 BX537631 D50927 BAA09486 DA144429 DN604386 DQ890904 ABM81830 DQ894057 ABM84983 Z25421 CAA80908 XM_005246981 XP_005247038 XM_011512238 XP_011510540 XR_923070 NM_001136554 NP_001130026 NM_001136555 NP_001130027 NM_012290 NP_036422 |
Function | Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S- phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'. {ECO:0000269|PubMed:10523312, ECO:0000269|PubMed:10588641, ECO:0000269|PubMed:11314006, ECO:0000269|PubMed:11470414, ECO:0000269|PubMed:12660173, ECO:0000269|PubMed:9427565}. |
Subcellular Location | Nucleus {ECO:0000269|PubMed:10523312, ECO:0000269|PubMed:10588641, ECO:0000269|PubMed:11314006, ECO:0000269|PubMed:9427565}. |
Tissue Specificity | Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined. {ECO:0000269|PubMed:10588641, ECO:0000269|PubMed:9427565}. |
TLK1 CRISPR/Cas9 KO Plasmid (h) - sc-407634 from Santa Cruz Biotechnology
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TLK1 HDR Plasmid (h) - sc-407634-HDR from Santa Cruz Biotechnology
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TLK1 Double Nickase Plasmid (h) - sc-407634-NIC from Santa Cruz Biotechnology
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TLK1 Double Nickase Plasmid (h2) - sc-407634-NIC-2 from Santa Cruz Biotechnology
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TLK1 CRISPR Activation Plasmid (h) - sc-407634-ACT from Santa Cruz Biotechnology
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TLK1 CRISPR Activation Plasmid (h2) - sc-407634-ACT-2 from Santa Cruz Biotechnology
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TLK1 Lentiviral Activation Particles (h) - sc-407634-LAC from Santa Cruz Biotechnology
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TLK1 Lentiviral Activation Particles (h2) - sc-407634-LAC-2 from Santa Cruz Biotechnology
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TLK1 siRNA (h) - sc-45973 from Santa Cruz Biotechnology
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TLK1 shRNA Plasmid (h) - sc-45973-SH from Santa Cruz Biotechnology
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TLK1 shRNA (h) Lentiviral Particles - sc-45973-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU115111 from Sigma-Aldrich
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MISSION® siRNA - SIHK2293 from Sigma-Aldrich
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MISSION® siRNA - SIHK2292 from Sigma-Aldrich
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MISSION® siRNA - SIHK2291 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN21394 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND21394 from Sigma-Aldrich
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TLK1 siRNA (Human) - CRH6622 from Cohesion Biosciences
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TLK1 RNAi - H00009874-R02 from Novus Biologicals
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TLK1 - MBS8237880 from MyBioSource
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TLK1 shRNA Plasmid (Human) - CHH6622 from Cohesion Biosciences
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TLK1 miRNA 3'UTR clone - MiUTR4H-TG09001 from Creative biogene
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