TLE3

Gene Symbol	TLE3
Entrez Gene	7090
Alt Symbol	ESG, ESG3, GRG3, HsT18976
Species	Human
Gene Type	protein-coding
Description	transducin-like enhancer of split 3
Other Description	enhancer of split groucho 3\|enhancer of split groucho-like protein 3\|transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)\|transducin-like enhancer of split 3, homolog of Drosophila E(sp1)\|transducin-like enhancer protein 3
Swissprots	Q8IVV6 F8W964 Q04726 E9PD64 Q8WVR2 Q9HCM5 Q6PI57 B4DPT0
Accessions	EAW77848 EAW77849 EAW77850 EAW77851 Q04726 AB046767 BAB13373 AB209672 BAD92909 AK000083 AK001258 BAG50879 AK096779 BAG53364 AK298482 BAG60692 AK304459 BAG65275 AK315058 AY566265 AAT72913 AY583459 AAS90945 AY583460 AAS90946 BC012004 BC015421 BC015729 BC041831 AAH41831 BC043247 AAH43247 BM676381 BP373048 M99438 AAA61194 XM_005254622 XP_005254679 XM_005254623 XP_005254680 XM_005254625 XP_005254682 XM_005254628 XP_005254685 XM_005254633 XP_005254690 XM_006720665 XP_006720728 XM_011521976 XP_011520278 XM_011521977 XP_011520279 XM_011521978 XP_011520280 XM_011521979 XP_011520281 XM_011521980 XP_011520282 XM_011521981 XP_011520283 XM_011521982 XP_011520284 XM_011521983 XP_011520285 NM_001105192 NP_001098662 NM_001282979 NP_001269908 NM_001282980 NP_001269909 NM_001282981 NP_001269910 NM_001282982 NP_001269911 NM_005078 NP_005069 NM_020908 NP_065959
Function	Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity). {ECO:0000250}.
Subcellular Location	Nucleus.
Tissue Specificity	Placenta and lung.

TLE3 (M-201) - sc-9124 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

TLE3 (M-201): sc-9124. Western blot analysis of TLE3 expression in A-431 (A) and HeLa (B) nuclear extracts.

TLE3 (N-20) - sc-13374 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

TLE3 (N-20): sc-13374. Western blot analysis of TLE3 expression in HEK293 whole cell lysate.

TLE3 (H-12) - sc-13375 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-TLE3 antibody - ab94972 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

TLE3 was immunoprecipitated using 0.5mg HeLa whole cell extract, 5µg of Rabbit polyclonal to TLE3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab94972.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 83kDa; TLE3

Anti-TLE3 antibody - ab32992 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-TLE3 antibody (ab32992) at 1/2.5 dilution + HepG2 cell lysateSecondary HRP conjugated anti Rabbit IgG at 1/50000 dilution

Anti-TLE3 antibody - ab185508 from Abcam

Prices	$443.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of paraffin-embedded Human vulva/anal skin tissue, labeling TLE3 using ab185508 at a 1/500 dilution.

Anti-TLE3 Antibody (aa295-311) - LS-C7310 from LifeSpan Bioscience

Prices	$325.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-TLE3 Antibody (aa161-210) - LS-C31410 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

TLE3 Antibody - AF5785 from Novus Biologicals

Prices	$99.00, $329.00
Sizes	25 µg, 100 µg
Host	Goat
Clonality	Polyclonal

Western blot shows lysates of MCF‑7 human breast cancer cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse TLE3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5785) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TLE3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

TLE3 Antibody - H00007090-B01P from Novus Biologicals

Prices	$359.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

TLE3 Antibody - NBP2-30646 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: TLE3 Antibody [NBP2-30646] - Analysis in human cell line HeLa nuclear extract.

Anti-Groucho3 antibody produced in rabbit - G3919 from Sigma-Aldrich

Prices	$340.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-TLE3 antibody produced in rabbit - AV32362 from Sigma-Aldrich

Prices	$267.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Cell Type: HepG2 (<B>Cat. No. AV32362</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 2.5 μg/mL in cell type HepG2

Anti-TLE3 antibody produced in rabbit - HPA054116 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-TLE3: <B>Cat. No. HPA054116</B>: Immunohistochemical staining of human vulva/anal skin shows strong nuclear positivity in epidermal cells.

TLE3 antibody - N-terminal region (ARP32362_T100) - ARP32362_T100 from Aviva Systems Biology

Prices	$229.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

TLE3-antibody-N-terminal-region - AI10053 from Abgent, a WuXi AppTec company

Prices	$232.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

TLE3 antibody - N-terminal region (AI10053) in Human HepG2 cells using Western BlotWB Suggested Anti-TLE3 Antibody Titration: 2.5ug/ml Positive Control: HepG2 cell lysate

Human/Mouse TLE3 Antibody - AF5785 from R&D Systems

Prices	$99.00, $329.00
Sizes	25 µg, 100 µg
Host	Goat

Detection of Human TLE3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse TLE3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5785) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109 ). A specific band was detected for TLE3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3 .

Human/Mouse TLE3 Affinity Purified Polyclonal Ab, Goat IgG - AF5785-SP from R&D Systems

Host	Goat
Clonality	Polyclonal

TLE3 antibody - 70R-20845 from Fitzgerald

Prices	$375.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

TLE3 Polyclonal Antibody - A4157 from ABclonal

Prices	$200.00, $480.00
Sizes	50 µg, 200 µg
Host	Rabbit
Clonality	Polyclonal

TLE3 antibody - orb230937 from Biorbyt

Prices	$323.00, $493.00
Sizes	100 μg, 200 μg
Host	Rabbit
Clonality	Polyclonal

TLE3 antibody (N-terminal) - 20R-3004 from Fitzgerald

Prices	$295.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

TLE3 Antibody - abx003071 from Abbexa

Host	Rabbit
Clonality	Polyclonal

TLE3 antibody - 11372-1-AP from Acris Antibodies

Prices	$270.00
Sizes	150 µl
Host	Rabbit
Clonality	Polyclonal

Hepg2 cell were subjected to SDS PAGE followed by western blot with 11372-1-AP(TLE3 Antibody) at dilution of 1:1200

TLE3 antibody - 22094-1-AP from Acris Antibodies

Prices	$270.00
Sizes	150 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry of paraffin-embedded human breast cancer slide using 22094-1-AP( TLE3 Antibody) at dilution of 1:50

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