| Gene Symbol | Piwil2 |
|---|---|
| Entrez Gene | 57746 |
| Alt Symbol | Piwil1l, mili |
| Species | Mouse |
| Gene Type | protein-coding |
| Description | piwi-like RNA-mediated gene silencing 2 |
| Other Description | Miwi like|Miwi likw|piwi like homolog 2|piwi-like homolog 2|piwi-like protein 2 |
| Swissprots | Q8CDG1 Q99MV6 Q3TQE8 Q9JMB6 A6H617 |
| Accessions | EDL35904 Q8CDG1 AB032605 BAA93706 AF285586 AAK31965 AK030116 BAC26791 AK047771 AK163647 BAE37436 BC138444 AAI38445 BC145717 AAI45718 XM_006519328 XP_006519391 XM_006519329 XP_006519392 NM_021308 NP_067283 |
| Function | Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer- independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piR |
| Subcellular Location | Cytoplasm {ECO:0000269|PubMed:11578866, ECO:0000269|PubMed:18922463, ECO:0000269|PubMed:19114715, ECO:0000269|PubMed:19465913, ECO:0000269|PubMed:19584108}. Note=Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. |
| Tissue Specificity | Expressed in adult testis, specifically in spermatocytes and in spermatogonia. Only detected in primordial germ cells of both sexes. Widely expressed in tumors. Also present at early stages of oocyte growth. Expressed in brain (at protein level). {ECO:0000269|PubMed:11279525, ECO:0000269|PubMed:11578866, ECO:0000269|PubMed:12906857, ECO:0000269|PubMed:18404146, ECO:0000269|PubMed:18922463, ECO:0000269|PubMed:19114715, ECO:0000269|PubMed:19377467}. |
| Top Pathways | Dorso-ventral axis formation |
MILI CRISPR/Cas9 KO Plasmid (m) - sc-425421 from Santa Cruz Biotechnology
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MILI HDR Plasmid (m) - sc-425421-HDR from Santa Cruz Biotechnology
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MILI Double Nickase Plasmid (m) - sc-425421-NIC from Santa Cruz Biotechnology
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MILI Double Nickase Plasmid (m2) - sc-425421-NIC-2 from Santa Cruz Biotechnology
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MILI CRISPR Activation Plasmid (m) - sc-425421-ACT from Santa Cruz Biotechnology
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MILI CRISPR Activation Plasmid (m2) - sc-425421-ACT-2 from Santa Cruz Biotechnology
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MILI Lentiviral Activation Particles (m) - sc-425421-LAC from Santa Cruz Biotechnology
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MILI Lentiviral Activation Particles (m2) - sc-425421-LAC-2 from Santa Cruz Biotechnology
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PIWIL2 siRNA (m) - sc-62457 from Santa Cruz Biotechnology
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PIWIL2 shRNA Plasmid (m) - sc-62457-SH from Santa Cruz Biotechnology
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PIWIL2 shRNA (m) Lentiviral Particles - sc-62457-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EMU008111 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN38782 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND38782 from Sigma-Aldrich
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PIWIL2 siRNA (Mouse) - CRM6038 from Cohesion Biosciences
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shRNA set against Mouse PIWIL2 (NM_021308.1) - SHH376300 from Creative biogene
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Mouse PIWIL2 siRNA - orb285514 from Biorbyt
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PIWIL2 shRNA Plasmid (Mouse) - CHM6038 from Cohesion Biosciences
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PIWIL2 miRNA 3'UTR clone - MiUTR1M-09252 from Creative biogene
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