HLCS

Gene Symbol HLCS
Entrez Gene 3141
Alt Symbol HCS
Species Human
Gene Type protein-coding
Description holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase)
Other Description biotin apo-protein ligase|biotin--[acetyl-CoA-carboxylase] ligase|biotin--[methylcrotonoyl-CoA-carboxylase] ligase|biotin--[methylmalonyl-CoA-carboxytransferase] ligase|biotin--protein ligase|holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)) ligase)
Swissprots B2RAH1 D3DSG6 P50747 Q99451
Accessions BAA89434 BAA95510 BAA95511 BAB68550 EAX09731 EAX09732 P50747 AJ001864 CAA05056 AK307940 AK314189 BAG36868 BC060787 AAH60787 BI094308 BQ775124 CB049233 D23672 BAA04902 D87328 BAA13332 DA334377 DB290499 H17102 X80160 XM_005260953 XP_005261010 XM_005260954 XP_005261011 XM_005260955 XP_005261012 XM_005260956 XP_005261013 XM_006723994 XP_006724057 XM_006723995 XP_006724058 XM_011529538 XP_011527840 XM_011529539 XP_011527841 XM_011529540 XP_011527842 XM_011529541 XP_011527843 XM_011529542 XP_011527844 NM_000411 NP_000402 NM_001242784 NP_001229713 NM_001242785 NP_001229714
Function Post-translational modification of specific protein by attachment of biotin. Acts on various carboxylases such as acetyl- CoA-carboxylase, pyruvate carboxylase, propionyl CoA carboxylase, and 3-methylcrotonyl CoA carboxylase.
Subcellular Location Cytoplasm. Mitochondrion.
Tissue Specificity Mostly expressed in muscle, placenta, in lesser extent in the brain, kidney, pancreas, liver and lung.
Top Pathways Biotin metabolism

HCS (N-19) - sc-23732 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HCS (N-19): sc-23732. Western blot analysis of HCS expression in JAR (A) and JEG-3 (B) whole cell lysates.

HCS (C-15) - sc-23734 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-HLCS antibody - ab100925 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HLCS antibody (ab100925) at 1/500 dilutionLane 1 : Human fetal lung lysate Lane 2 : Human fetal kidney lysate

Anti-HLCS antibody - ab70085 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-HLCS antibody (ab70085) at 1/500 dilutionLane 1 : HLCS transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutiondeveloped using the ECL technique

Anti-HLCS antibody [EPR8510] - ab137126 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8510
All lanes : Anti-HLCS antibody [EPR8510] (ab137126) at 1/1000 dilutionLane 1 : JAR lysateLane 2 : Human skeletal muscle lysateLane 3 : Human fetal lung lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-HLCS antibody - N-terminal - ab186124 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HLCS antibody - N-terminal (ab186124) at 0.4 µg/mlLane 1 : HeLa whole cell lysateLane 2 : 293T whole cell lysateLane 3 : Jurkat whole cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique

Anti-HLCS / HCS Antibody - LS-C308263 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-HLCS / HCS Antibody - LS-C137344 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HLCS / HCS Antibody - LS-C311228 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HLCS / HCS Antibody - LS-C311229 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-HLCS / HCS Antibody (aa1-50) - LS-C290902 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human HCS by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HCS antibody used for IP at 6 ug per reaction. HCS was also immunoprecipitated by rabbit anti-HCS antibody BL15481. For blotting immunoprecipitated HCS, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-HLCS / HCS Antibody (clone 1E4) - LS-C173805 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1E4
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-HLCS mouse monoclonal antibody.

HLCS Antibody - NBP1-81759 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: HLCS Antibody [NBP1-81759] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

HLCS Antibody (1E4) - NBP2-01859 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1E4
Western Blot: HLCS Antibody (1E4) [NBP2-01859] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLCS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HLCS.

HLCS Antibody - NBP2-16856 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: HLCS Antibody [NBP2-16856] - Sample (30 ug of whole cell lysate) A: K562 7. 5% SDS PAGE gel, diluted at 1:2000.

HLCS Antibody - NBP2-32174 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: HLCS Antibody [NBP2-32174] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-HLCS antibody NBP2-32174 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

HLCS Antibody - H00003141-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: HLCS Antibody [H00003141-B01P] - Analysis of HLCS expression in transfected 293T cell line by HLCS polyclonal antibody. Lane1:HLCS transfected lysate(79.86 KDa). Lane2:Non-transfected lysate.

HLCS Antibody - H00003141-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

HLCS Antibody - H00003141-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: HLCS Antibody [H00003141-D01P] - Analysis of HLCS expression in transfected 293T cell line by HLCS polyclonal antibody.Lane 1: HLCS transfected lysate(80.80 KDa).Lane 2: Non-transfected lysate.

HLCS Antibody - H00003141-D01 from Novus Biologicals

Host Rabbit
Clonality Polyclonal

Anti-HLCS antibody produced in rabbit - SAB1410637 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-HLCS: <B>Cat. No. SAB1410637</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~80.8 kDa using HLCS transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-HLCS antibody produced in rabbit - HPA017379 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-HLCS: <B>Cat. No. HPA017379</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney shows granular cytoplasmic positivity in tubular cells.

Biotin Antibody (OAAI00293) - OAAI00293 from Aviva Systems Biology

Prices $255.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

HLCS Antibody - C-terminal region (ARP74943_P050) - ARP74943_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

HLCS antibody - orb77697 from Biorbyt

Prices $425.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed paraffin embedded fetal skeletal muscle showing cytoplasmic staining using HLCS antibody
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