| Gene Symbol | STT3B |
|---|---|
| Entrez Gene | 201595 |
| Alt Symbol | CDG1X, SIMP, STT3-B |
| Species | Human |
| Gene Type | protein-coding |
| Description | STT3B, subunit of the oligosaccharyltransferase complex (catalytic) |
| Other Description | STT3, subunit of the oligosaccharyltransferase complex, homolog B|dolichyl-diphosphooligosaccharide protein glycotransferase|dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B|homolog of yeast STT3|oligosaccharyl transferase subunit STT3B|source of immunodominant MHC-associated peptides homolog |
| Swissprots | Q96JZ4 Q96KY7 Q8TCJ2 |
| Accessions | CAF85831 EAW64415 EAW64416 Q8TCJ2 AI914841 AK027789 BAB55370 AK074587 AK075380 BAC11581 AK311488 AX799082 CAE48447 AY074880 AAL71884 BC015880 AAH15880 HY015137 HY039873 XM_011533465 XP_011531767 NM_178862 NP_849193 |
| Function | Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N-glycosylation of target proteins: STT3B-containing complexes are required for efficient cotranslational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A. STT3B-containing complexes also act post-translationally and mediate modification of skip |
| Subcellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| Tissue Specificity | Expressed in heart, brain, placenta, lung, liver, muscle, kidney and pancreas. Expressed in skin fibroblasts (at protein level). {ECO:0000269|PubMed:12887896}. |
| Top Pathways | N-Glycan biosynthesis, Protein processing in endoplasmic reticulum |
SIMP CRISPR/Cas9 KO Plasmid (h) - sc-404481 from Santa Cruz Biotechnology
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SIMP HDR Plasmid (h) - sc-404481-HDR from Santa Cruz Biotechnology
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SIMP Double Nickase Plasmid (h) - sc-404481-NIC from Santa Cruz Biotechnology
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SIMP Double Nickase Plasmid (h2) - sc-404481-NIC-2 from Santa Cruz Biotechnology
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SIMP CRISPR Activation Plasmid (h) - sc-404481-ACT from Santa Cruz Biotechnology
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SIMP CRISPR Activation Plasmid (h2) - sc-404481-ACT-2 from Santa Cruz Biotechnology
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SIMP Lentiviral Activation Particles (h) - sc-404481-LAC from Santa Cruz Biotechnology
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SIMP Lentiviral Activation Particles (h2) - sc-404481-LAC-2 from Santa Cruz Biotechnology
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SIMP siRNA (h) - sc-78489 from Santa Cruz Biotechnology
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SIMP shRNA Plasmid (h) - sc-78489-SH from Santa Cruz Biotechnology
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SIMP shRNA (h) Lentiviral Particles - sc-78489-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU051881 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN20773 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND20773 from Sigma-Aldrich
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STT3B siRNA (Human) - CRJ6298 from Cohesion Biosciences
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STT3B - MBS8229432 from MyBioSource
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shRNA set against Human STT3B (NM_178862.1) - SHH420216 from Creative biogene
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shRNA set against Human STT3B(NM_178862.1) - SHL045264 from Creative biogene
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STT3B shRNA Plasmid (Human) - CHJ6298 from Cohesion Biosciences
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STT3B miRNA 3'UTR clone - MiUTR1H-10033 from Creative biogene
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