EBF2

Gene Symbol	EBF2
Entrez Gene	64641
Alt Symbol	COE2, EBF-2, O/E-3, OE-3
Species	Human
Gene Type	protein-coding
Description	early B-cell factor 2
Other Description	Collier, Olf and EBF 2\|OLF-1/EBF-LIKE 3\|metencephalon-mesencephalnon-olfactory transcription factor 1\|transcription factor COE2
Swissprots	Q6ISA4 F5H645 A0PJM4 Q9HAK2 Q6IS86 A6NMF7 Q66VZ3 Q6DK36
Accessions	Q9HAK2 AI457889 AK001144 AK021562 BAB13843 AK093878 AK304372 BAH14170 AL701879 AU120738 AY700779 AAU10086 BC069665 AAH69665 BC069726 AAH69726 BC069747 AAH69747 BC069768 AAH69768 BC074794 AAH74794 BC113478 AAI13479 BC113504 AAI13505 BC137106 AAI37107 BC137107 AAI37108 BC148638 AAI48639 BQ878633 BY798905 NM_022659 NP_073150
Function	Transcription factor that, in osteoblasts, activates the decoy receptor for RANKL, TNFRSF11B, which in turn regulates osteoclast differentiation. Acts in synergy with the Wnt- responsive LEF1/CTNNB1 pathway. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' (By similarity). {ECO:0000250}.
Subcellular Location	Nucleus {ECO:0000305}.

EBF2 (S-23) - sc-133528 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

EBF2 (S-23): sc-133528. Western blot analysis of EBF2 expression in Hep G2 whole cell lysate.

Anti-EBF2 antibody - ab156999 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Total extracts from Human Jurkat cells were immunoprecipitated (IP) with IgG (Lane 1) or anti-EBF2 (ab156999) (Lane 2); followed by WB with ab156999 and Rabbit IgG light chain-specific secondard antibody.

Anti-EBF2 antibody - ab56048 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-EBF2 antibody (ab56048) at 1.25 µg/ml + HepG2 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-EBF2 antibody - ab94791 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-EBF2 antibody (ab94791) at 1 µg/ml (in 5% skim milk / PBS buffer) + ACHN cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-EBF2 Antibody (aa407-519) - LS-C182839 from LifeSpan Bioscience

Prices	$650.00
Sizes	100 µg
Host	Sheep
Clonality	Polyclonal

Anti-EBF2 Antibody (aa405-454) - LS-C31776 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-EBF2 Antibody (aa285-536, Biotin) - LS-C299493 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-EBF2 Antibody (aa285-536, FITC) - LS-C303195 from LifeSpan Bioscience

Prices	$435.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-EBF2 Antibody (aa359-408) - LS-C110106 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

EBF2 antibody LS-C110106 Western blot of ACHN lysate.

Anti-EBF2 Antibody (aa249-298) - LS-C205690 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

EBF2 antibody Western Blot of Thymus Tumor. Antibody dilution: 1 ug/ml.

Anti-EBF2 Antibody (aa285-536) - LS-C293716 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

EBF-2 Antibody - AF7006 from Novus Biologicals

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Sheep
Clonality	Polyclonal

Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7006) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EBF-2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

EBF2 Polyclonal Antibody - bs-11740R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EBF2 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-11740R-A350 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EBF2 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-11740R-A488 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EBF2 Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated - bs-11740R-A555 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EBF2 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated - bs-11740R-A647 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EBF2 Polyclonal Antibody, Cy3 Conjugated - bs-11740R-Cy3 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EBF2 Polyclonal Antibody, Cy5 Conjugated - bs-11740R-Cy5 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EBF2 Polyclonal Antibody, Cy5.5 Conjugated - bs-11740R-Cy5.5 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EBF2 Polyclonal Antibody, Cy7 Conjugated - bs-11740R-Cy7 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EBF2 Polyclonal Antibody, FITC Conjugated - bs-11740R-FITC from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EBF2 Polyclonal Antibody, Biotin Conjugated - bs-11740R-Biotin from Bioss

Prices	$349.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EBF2 Polyclonal Antibody, HRP Conjugated - bs-11740R-HRP from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-EBF2 antibody produced in rabbit - E8533 from Sigma-Aldrich

Prices	$57.50, $332.50
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Lysate of HEK-293T over expressing human EBF2 was separated on SDS-PAGE and probed with Rabbit Anti-EBF2 (<B>Cat. No. E8533</B>). The antibody was developed with Goat anti-Rabbit IgG, Peroxidase conjugate (<B>Cat. No. A0545</B>) and a chemiluminescence substrate. <BR/><B>Lanes</B><BR/>1. 1 μg/ML Anti-EBF2 <BR/>2. 1 μg/mL Anti-EBF2 + 20 μg immunizing peptide

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