PHB

Gene Symbol PHB
Entrez Gene 5245
Alt Symbol HEL-215, HEL-S-54e, PHB1
Species Human
Gene Type protein-coding
Description prohibitin
Other Description epididymis luminal protein 215|epididymis secretory sperm binding protein Li 54e
Swissprots P35232 B4DY47 Q4VBQ0
Accessions AAA86691 AAO18340 CAL48156 EAW94680 EAW94681 P35232 AA357151 AA709387 AK223181 BAD96901 AK290766 BAF83455 AK302258 BAG63609 AK307767 AK312649 BAG35532 AM393620 CAL38496 AY577780 AAS88903 BC013401 AAH13401 BC095460 AAH95460 BE729762 BF038604 BG029858 BI871461 BM972128 BP267961 BQ574485 BT007411 AAP36079 CR541706 CAG46507 CX784018 DC360133 FJ224341 ACI46033 FJ460516 ACS44653 S85655 AAB21614 NM_001281496 NP_001268425 NM_001281497 NP_001268426 NM_001281715 NP_001268644 NM_002634 NP_002625
Function Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. {ECO:0000269|PubMed:11302691}.
Subcellular Location Mitochondrion inner membrane {ECO:0000250}.
Tissue Specificity Widely expressed in different tissues.

PHB1 Antibody - 2426 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from MCF-7, Neuro2A, PC12 and COS cells, using PHB1 Antibody.

Prohibitin (E-5) - sc-377037 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Prohibitin (E-5): sc-377037. Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing cytoplasmic and nuclear staining of follicle cells and ovarian stroma cells.

Prohibitin (H-80) - sc-28259 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Prohibitin (H-80): sc-28259. Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization.

Prohibitin (6K2D1) - sc-53996 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Prohibitin (C-13) - sc-18198 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Prohibitin (C-13): sc-18198. Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization.

Prohibitin (N-20) - sc-18196 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Prohibitin (N-20): sc-18196. Western blot analysis of Prohibitin expression in A-431 (A), Ramos (B), F9 (C) and Raji (D) whole cell lysates.

Prohibitin (SPM311) - sc-56467 from Santa Cruz Biotechnology

Prices $279.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Prohibitin (SPM311): sc-56467. Western blot analysis of Prohibitin expression in F9 (A), RAW 264.7 (B), A-10 (C) and NIH/3T3 (D) whole cell lysates and rat liver (E) and mouse liver (F) tissue extracts.

Anti-Prohibitin antibody - ab55618 from Abcam

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
ICC/IF image of rat primary mixed glial culture stained with ab55618. The cells on cover slip were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3x triton1000 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab55618, 1µg/ml) overnight at +4°C. The secondary antibody was Alexa Fluor®568 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.See Abreview

Anti-Prohibitin antibody - ab154589 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Prohibitin antibody (ab154589) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

Anti-Prohibitin antibody - ab70672 from Abcam

Prices $376.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
ab70672, at 1/100 dilution, staining Prohibitin in formalin fixed, paraffin embedded human heart tissue by immunohistochemistry.

Anti-Prohibitin antibody - ab115669 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Human Kidney (formalin-fixed, paraffin-embedded) stained with ab115669 at 1/50 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Prohibitin antibody [EP2803Y] - ab75766 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2803Y
Overlay histogram showing HepG2 cells stained with ab75766 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75766, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker - ab75771 from Abcam

Prices $388.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2804Y
All lanes : Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771) at 1/1000 dilutionLane 1 : MCF-7 cell lysatesLane 2 : PC-12 cell lysatesLane 3 : A431 cell lysatesLane 4 : Ramos cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (Alexa Fluor® 488) - ab184813 from Abcam

Prices $433.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2804Y
ab184813 staining Prohibitin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab184813 at a working dilution of 1 in 50 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at a dilution of 1 in 250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Prohibitin antibody [II-14-10] - ab1836 from Abcam

Prices $403.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone II-14-10
Formalin fixed paraffin embedded human heart stained with Prohibitin antibody ab1836.

Anti-Prohibitin antibody [II-14-10] (Biotin) - ab79198 from Abcam

Prices $372.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone II-14-10

Anti-Prohibitin antibody, prediluted - ab74539 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ab74539 staining Prohibitin in Human Heart by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

Anti-Prohibitin antibody [SPM311], prediluted - ab54330 from Abcam

Prices $376.00
Sizes 7 ml
Host Mouse
Clonality Monoclonal
Clone SPM311
Ab54330 ready to use staining human heart tissue; formalin fixed, paraffin embedded.

Anti-Prohibitin antibody - Mitochondrial Marker - ab28172 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab28172 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab28172, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence.  5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

Anti-PHB / Prohibitin Antibody - LS-C38346 from LifeSpan Bioscience

Prices $465.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal

Anti-PHB / Prohibitin Antibody (clone II-14-10) - LS-C85386 from LifeSpan Bioscience

Prices $650.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone II-14-10

Anti-PHB / Prohibitin Antibody (clone II-14-10, Biotin) - LS-C86887 from LifeSpan Bioscience

Prices $785.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone II-14-10

Anti-PHB / Prohibitin Antibody (clone II-14-10, Biotin) - LS-C87992 from LifeSpan Bioscience

Prices $320.00, $750.00, $1,160.00
Sizes 20 µg, 100 µg, 1 ml
Host Mouse
Clonality Monoclonal
Clone II-14-10

Anti-PHB / Prohibitin Antibody (clone II-14-10, Azide-free) - LS-C87993 from LifeSpan Bioscience

Prices $630.00, $990.00
Sizes 100 µg, 200 µg
Host Mouse
Clonality Monoclonal
Clone II-14-10

Anti-PHB / Prohibitin Antibody - LS-C89326 from LifeSpan Bioscience

Prices $575.00
Sizes 6 ml
Host Mouse
Clonality Monoclonal
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