DAXX

Gene Symbol DAXX
Entrez Gene 1616
Alt Symbol BING2, DAP6, EAP1
Species Human
Gene Type protein-coding
Description death-domain associated protein
Other Description CENP-C binding protein|ETS1-associated protein 1|Fas-binding protein|death domain-associated protein 6|death-associated protein 6|fas death domain-associated protein
Swissprots Q5STK9 F5ANJ6 O14747 Q9BWI3 Q9UER7 O15141 O15208 F5ANJ7 B4E1I3 F5H082
Accessions CAB09986 CAB09989 EAX03722 EAX03723 EAX03724 Q9UER7 AA533586 AB209493 BAD92730 AF006041 AAB63043 AF015956 AAB66585 AF039136 AAB92671 AF050179 AAC39853 AF097742 AAC72843 AK223404 BAD97124 AK292187 BAF84876 AK303767 BAG64733 AK303854 BAG64795 AK309612 AK313034 BAG35867 BC000220 AAH00220 BC073776 BC109073 AAI09074 BC109074 AAI09075 BM997178 CR457085 CAG33366 DC345308 DC359186 EU446949 ABZ92478 HQ436528 AEC33235 HQ436529 AEC33236 XM_005248860 XP_005248917 XM_005275218 XP_005275275 XM_005275354 XP_005275411 NM_001141969 NP_001135441 NM_001141970 NP_001135442 NM_001254717 NP_001241646 NM_001350 NP_001341
Function Transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Inhibits transcriptional activatiopn of PAX3 and ETS1 through direct protein-protein interactions. Modulates PAX5 activity; the function seems to involve CREBBP. Acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination
Subcellular Location Isoform gamma: Nucleus {ECO:0000269|PubMed:21482821}. Note=Diffuse nuclear distribution pattern and no comparable dot-like accumulation of isoform 1. {ECO:0000269|PubMed:21482821}.
Tissue Specificity Ubiquitous.
Top Pathways Amyotrophic lateral sclerosis (ALS), MAPK signaling pathway, Herpes simplex infection

Daxx (25C12) Rabbit mAb - 4533 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 25C12
Western blot analysis of extracts from K562 (human), A20 (mouse) and PC12 (rat) cell lines using Daxx (25C12) Rabbit mAb.

Daxx Antibody - 4532 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Ramos and THP-1 cells, using Daxx Antibody.

Daxx (C-20) - sc-7000 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Daxx (C-20): sc-7000. Western blot analysis of Daxx expression in MOLT-4 (A) and Ramos (B) whole cell lysates.

Daxx (H-7) - sc-8043 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Daxx (H-7): sc-8043. Western blot analysis of Daxx expression in MOLT-4 (A), Ramos (B) and RAW 264.7 (C) whole cell lysates.

Daxx (M-112) - sc-7152 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Daxx (M-112): sc-7152. Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing nuclear localization.

Daxx (S-20) - sc-7001 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Daxx (S-20): sc-7001. Western blot analysis of Daxx expression in MOLT-4 (A) and Ramos (B) whole cell lysates.

Daxx (DAXX-01) - sc-51586 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Daxx (DAXX-01): sc-51586. Western blot analysis of Daxx expression in HeLa (A), MOLT-4 (B), Ramos (C), K-562 (D) and Raji (E) whole cell lysates.

Daxx (6D345) - sc-70952 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Daxx (6D345): sc-70952. Western blot analysis of Daxx expression in HeLa (A), MOLT-4 (B), Ramos (C) and K-562 (D) whole cell lysates.

p-Daxx (Ser 213) - sc-130187 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Daxx (Ser 213): sc-130187. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.

p-Daxx (Ser 671) - sc-130592 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-Daxx (phospho S668) antibody - ab55323 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Daxx (phospho S668) antibody (ab55323) at 1/500 dilutionLane 1 : Extracts from 293 cells treated with PBS for 60 minutes.Lane 2 : Extracts from 293 cells treated with PBS for 60 minutes, and with the immunising phosphopeptide.

Anti-Daxx antibody - ab105173 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab105173 (1/200) staining DAXX in asynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.See Abreview

Anti-Daxx antibody - ab47833 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Daxx antibody (ab47833) at 1/200 dilutionLane 1 : RWPE-1 cell extract at 25 µgLane 2 : RWPE-1 cell extract at 50 µgSecondaryPeroxidase conjugated anti-Rabbit IgG at 1/25000 dilution

Anti-Daxx antibody - ab2017 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab2017 at 10µg/ml staining DAXX in Hela cells by ICC/IF

Anti-Daxx antibody - ab151954 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Daxx antibody (ab151954) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : H1299 whole cell lysateLane 3 : Raji whole cell lysateLysates/proteins at 30 µg per lane.

Anti-Daxx antibody - ab66238 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
All lanes : Anti-Daxx antibody (ab66238) at 1 µg/mlLane 1 : Daxx transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 50 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-Daxx antibody - ab69802 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Daxx antibody (ab69802) at 1/500 dilutionLane 1 : extracts from 293 cellsLane 2 : extracts from A549 cellsLane 3 : extracts from A549 cells plus immunizing peptideLysates/proteins at 5 µg per lane.

Anti-Daxx antibody - ab133220 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin embedded Human small intestine tissue labelling Daxx with ab133220 at 15 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Daxx antibody - ab55370 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Daxx antibody (ab55370) at 1/500 dilutionLane 1 : extracts from COS7 cells, treated with Serum (10% for 30 mins) with no immunizing peptideLane 2 : extracts from COS7 cells, treated with Serum (10% for 30 mins) with immunizing peptide

Anti-Daxx antibody [7A11] - ab130198 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7A11
All lanes : Anti-Daxx antibody [7A11] (ab130198) at 1/500 dilutionLane 1 : K562 cell lysateLane 2 : β-actin

Anti-Daxx antibody [DAXX-01] - ab9091 from Abcam

Prices $379.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone DAXX-01
ICC/IF image of ab9091 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9091, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Daxx antibody [E94] - ab32140 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E94
ab32140 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32140 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-Daxx antibody [E94] (Alexa Fluor® 647) - ab202831 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E94
ab202831 staining Daxx in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202831 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-DAXX Antibody (Ser671, FITC) - LS-C276655 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DAXX Antibody (Ser671, Azide-free, HRP) - LS-C276656 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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