RACGAP1
| Gene Symbol | RACGAP1 |
|---|---|
| Entrez Gene | 29127 |
| Alt Symbol | CYK4, HsCYK-4, ID-GAP, MgcRacGAP |
| Species | Human |
| Gene Type | protein-coding |
| Description | Rac GTPase activating protein 1 |
| Other Description | GTPase activating protein|male germ cell RacGap|protein CYK4 homolg|protein CYK4 homolog|rac GTPase-activating protein 1 |
| Swissprots | Q9P2W2 Q9NWN2 Q9H0H5 Q6PJ26 Q9P250 |
| Accessions | EAW58113 EAW58114 EAW58115 Q9H0H5 AB030251 BAA90247 AB040911 BAA96002 AK000733 BAA91347 AK022726 BAB14206 AK225901 AK315785 BAG38131 AL136794 CAB66728 AM393855 CAL38730 BC024144 AAH24144 BC032754 AAH32754 CR533565 CAG38596 DA441098 DB076571 DQ894409 ABM85335 EU176264 ABW03715 XM_005268812 XP_005268869 XM_005268813 XP_005268870 XM_005268814 XP_005268871 XM_005268815 XP_005268872 XM_006719359 XP_006719422 XM_011538235 XP_011536537 XM_011538236 XP_011536538 XM_011538237 XP_011536539 XM_011538238 XP_011536540 XM_011538239 XP_011536541 XM_011538240 XP_011536542 XM_011538241 XP_011536543 XM_011538242 XP_011536544 XM_011538243 XP_011536545 NM_001126103 NP_001119575 NM_001126104 NP_001119576 NM_013277 NP_037409 |
| Function | Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells. {ECO:0000269|PubMed:10979956, ECO:0000269|PubMed:11085985, ECO:0000269|PubMed:11278976 |
| Subcellular Location | Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasmic vesicle, secretory vesicle, acrosome. Cleavage furrow. Midbody. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Colocalizes with RND2 in Golgi- derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase. {ECO:0000250}. |
| Tissue Specificity | Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase. {ECO:0000269|PubMed:10979956, ECO:0000269|PubMed:11278976, ECO:0000269|PubMed:12590651, ECO:0000269|PubMed:9497316}. |
Rac GAP1 CRISPR/Cas9 KO Plasmid (h) - sc-402969 from Santa Cruz Biotechnology
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Rac GAP1 HDR Plasmid (h) - sc-402969-HDR from Santa Cruz Biotechnology
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Rac GAP1 Double Nickase Plasmid (h) - sc-402969-NIC from Santa Cruz Biotechnology
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Rac GAP1 Double Nickase Plasmid (h2) - sc-402969-NIC-2 from Santa Cruz Biotechnology
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Rac GAP1 CRISPR Activation Plasmid (h) - sc-402969-ACT from Santa Cruz Biotechnology
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Rac GAP1 CRISPR Activation Plasmid (h2) - sc-402969-ACT-2 from Santa Cruz Biotechnology
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Rac GAP1 Lentiviral Activation Particles (h) - sc-402969-LAC from Santa Cruz Biotechnology
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Rac GAP1 Lentiviral Activation Particles (h2) - sc-402969-LAC-2 from Santa Cruz Biotechnology
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Rac GAP1 siRNA (h) - sc-76335 from Santa Cruz Biotechnology
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Rac GAP1 shRNA Plasmid (h) - sc-76335-SH from Santa Cruz Biotechnology
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Rac GAP1 shRNA (h) Lentiviral Particles - sc-76335-V from Santa Cruz Biotechnology
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MgcRacGAP/RACGAP1 RNAi - H00029127-R01 from Novus Biologicals
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MISSION® esiRNA - EHU081301 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17888 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17888 from Sigma-Aldrich
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RACGAP1 siRNA (Human) - CRH9215 from Cohesion Biosciences
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RACGAP1 - MBS8229072 from MyBioSource
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Human RACGAP1 siRNA - orb269717 from Biorbyt
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RACGAP1 shRNA Plasmid (Human) - CHH9215 from Cohesion Biosciences
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RACGAP1 miRNA 3'UTR clone - MiUTR3H-07264 from Creative biogene
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RACGAP1 miRNA 3'UTR clone - MiUTR3H-07265 from Creative biogene
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RACGAP1 miRNA 3'UTR clone - MiUTR3H-07266 from Creative biogene
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