NSF

Gene Symbol NSF
Entrez Gene 4905
Alt Symbol SKD2
Species Human
Gene Type protein-coding
Description N-ethylmaleimide-sensitive factor
Other Description N-ethylmaleimide-sensitive factor-like protein|N-ethylmaleimide-sensitive fusion protein|NEM-sensitive fusion protein|vesicle-fusing ATPase|vesicular-fusion protein NSF
Swissprots B4DFA2 Q9UKZ2 Q8N6D7 A8K2D9 P46459
Accessions CAH56804 EAW57706 EAW57707 P46459 AF102846 AAF04745 AF135168 AAF70545 AK226078 AK290204 BAF82893 AK294001 BAG57363 AK294727 BAG57874 AK294886 BAG57980 AK299031 BAH12933 AK299786 BAG61667 AK316057 BAH14428 AL049276 BC013314 AAH13314 BC030613 AAH30613 BM451383 DB460965 U03985 AAA17411 U80997 AAG23890 XM_011524863 XP_011523165 XM_011546358 XP_011544660 XM_011547488 XP_011545790 NM_006178 NP_006169 NR_040116
Function Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm.
Top Pathways Vasopressin-regulated water reabsorption, Synaptic vesicle cycle, GABAergic synapse

NSF (D31C7) XP ® Rabbit mAb - 3924 from Cell Signaling Technology

Prices $277.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D31C7
Western blot analysis of extracts from various cell types using NSF (D31C7) XP ® Rabbit mAb.

NSF Antibody - 2145 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SHSY5Y, Neuro2A, PC12 and CHO cells using NSF Antibody.

NSF (H-300) - sc-15339 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NSF (H-300): sc-15339. Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

NSF (N-18) - sc-15915 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NSF (N-18): sc-15915. Western blot analysis of NSF expression in EOC 20 whole cell lysate.

NSF (C-5) - sc-74457 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NSF (C-5): sc-74457. Western blot analysis of NSF expression in SK-N-SH (A), SH-SY5Y (B) and KNRK (C) whole cell lysates.

NSF (C-19) - sc-15917 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NSF (C-19): sc-15917. Western blot analysis of NSF expression in mouse brain tissue extract.

NSF (E-6) - sc-74458 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NSF (E-6): sc-74458. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

NSF (7) - sc-136008 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
NSF (7): sc-136008. Western blot analysis of NSF expression in HeLa whole cell lysate.

Anti-NSF antibody - ab87155 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NSF antibody (ab87155) at 1 µg/mlLane 1 : Brain (Rat) Tissue LysateLane 2 : Rat Hippocampus Tissue LysateLane 3 : Brain (Mouse) Tissue LysateLane 4 : Mouse Hippocampus Tissue LysateLane 5 : CHO-K1 cell lysate Whole Cell LysateLane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-NSF antibody - ab126202 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-NSF antibody (ab126202) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

Anti-NSF antibody [9G7-3] - ab12368 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 9G7-3
All lanes : Anti-NSF antibody [9G7-3] (ab12368) at 1/1000 dilutionLane 1 : Molecular weight markerLane 2 : Lysates prepared from heat shocked Hela cellsLane 3 : Lysates prepared from Vero cellsLane 4 : Lysates prepared from CHO-K1 cellsLane 5 : Lysates prepared from ESK-4 cells

Anti-NSF antibody [NSF-1] - ab16681 from Abcam

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone NSF-1
All lanes : Anti-NSF antibody [NSF-1] (ab16681) at 0.5 µg/mlLane 1 : 3T3Lane 2 : PC12Lane 3 : MDBKLane 4 : Normal human fibroblasts

Anti-NSF antibody - C-terminal - ab156598 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NSF antibody - C-terminal (ab156598) at 1/100 dilution + Mouse cerebellum tissue lysate at 35 µg

Anti-NSF Antibody - LS-C9353 from LifeSpan Bioscience

Prices $375.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-NSF Antibody (Leu524) - LS-C37801 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NSF Antibody - LS-C17877 from LifeSpan Bioscience

Prices $335.00
Sizes 25 µg
Host Mouse
Clonality Monoclonal

Anti-NSF Antibody (N-Terminus) - LS-C94343 from LifeSpan Bioscience

Prices $325.00
Sizes 500 µg
Host Rabbit
Clonality Polyclonal

Anti-NSF Antibody (aa668-696, AP) - LS-C207357 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSF Antibody (aa668-696, APC) - LS-C207358 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSF Antibody (aa668-696, Biotin) - LS-C207359 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSF Antibody (aa668-696, FITC) - LS-C207360 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSF Antibody - LS-C26575 from LifeSpan Bioscience

Prices $285.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot (10% SDS-PAGE) of 3T3 (lane 1), PC12 (lane 2), MDBK (lane 3) and normal human fibroblasts (lane 4) probed with clone NSF-1 anti-NSF at 0.5 g/ml. The diffuse bands at ~70 kDa were also present on a blot incubated with second antibody alone (data not shown).

Anti-NSF Antibody (aa668-696, Azide-free, HRP) - LS-C207361 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSF Antibody (aa668-696, PE) - LS-C207363 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSF Antibody (aa157-202) - LS-C313863 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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