Gene Symbol | DNAJA1 |
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Entrez Gene | 3301 |
Alt Symbol | DJ-2, DjA1, HDJ2, HSDJ, HSJ2, HSPF4, NEDD7, hDJ-2 |
Species | Human |
Gene Type | protein-coding |
Description | DnaJ (Hsp40) homolog, subfamily A, member 1 |
Other Description | HSJ-2|dnaJ homolog subfamily A member 1|dnaJ protein homolog 2|heat shock 40 kDa protein 4|heat shock protein J2|heat shock protein, DNAJ-like 2|human DnaJ protein 2|neural precursor cell expressed, developmentally down-regulated 7 |
Swissprots | Q86TL9 Q5T7Q0 P31689 |
Accessions | EAW58524 EAW58525 EAW58526 EAW58527 P31689 AJ277242 CAB93148 AK289623 BAF82312 AK298725 BAH12856 AY186741 AAO31694 BC008182 AAH08182 BI462555 BT007292 AAP35956 D13388 BAA02656 DQ891091 ABM82017 EU176556 ABW03357 L08069 AAC37517 NM_001539 NP_001530 |
Function | Co-chaperone for HSPA8/Hsc70 (PubMed:10816573). Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877). Plays a role in protein transport into mitochondria via its role as co- chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed:14752510). Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed:24512202). {ECO:0000269|PubMed:10816573, ECO:0000269|PubMed:14752510, ECO:0000269|PubMed:24318877, ECO:0000269|PubMed:24512202, ECO:0000269|PubMed:9192730}. |
Subcellular Location | Membrane {ECO:0000305|PubMed:10816573}; Lipid-anchor {ECO:0000305|PubMed:10816573}. Cytoplasm {ECO:0000269|PubMed:10816573}. Microsome {ECO:0000250}. Nucleus {ECO:0000269|PubMed:10816573}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:10816573}. Mitochondrion {ECO:0000250}. Note=Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei. {ECO:0000250}. |
Tissue Specificity | Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver. {ECO:0000269|PubMed:10816573, ECO:0000269|PubMed:15595953}. |
Top Pathways | Protein processing in endoplasmic reticulum |
HSP 40-4 CRISPR/Cas9 KO Plasmid (h) - sc-403819 from Santa Cruz Biotechnology
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HSP 40-4 HDR Plasmid (h) - sc-403819-HDR from Santa Cruz Biotechnology
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HSP 40-4 Double Nickase Plasmid (h) - sc-403819-NIC from Santa Cruz Biotechnology
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HSP 40-4 Double Nickase Plasmid (h2) - sc-403819-NIC-2 from Santa Cruz Biotechnology
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HSP 40-4 CRISPR Activation Plasmid (h) - sc-403819-ACT from Santa Cruz Biotechnology
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HSP 40-4 CRISPR Activation Plasmid (h2) - sc-403819-ACT-2 from Santa Cruz Biotechnology
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HSP 40-4 Lentiviral Activation Particles (h) - sc-403819-LAC from Santa Cruz Biotechnology
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HSP 40-4 Lentiviral Activation Particles (h2) - sc-403819-LAC-2 from Santa Cruz Biotechnology
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HSP 40-4 siRNA (h) - sc-60816 from Santa Cruz Biotechnology
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HSP 40-4 shRNA Plasmid (h) - sc-60816-SH from Santa Cruz Biotechnology
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HSP 40-4 shRNA (h) Lentiviral Particles - sc-60816-V from Santa Cruz Biotechnology
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HDJ2 RNAi - H00003301-R01 from Novus Biologicals
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MISSION® esiRNA - EHU114481 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN7340 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND7340 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR01291 from Sigma-Aldrich
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DNAJA1 siRNA (Human) - CRH2257 from Cohesion Biosciences
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DNAJA1 - MBS8231466 from MyBioSource
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HSP 40-4 CRISPR/Cas9 KO Plasmid (h2) - sc-403819-KO-2 from Santa Cruz Biotechnology
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HSP 40-4 HDR Plasmid (h2) - sc-403819-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human DNAJA1 (NM_001539.2) - SHH279213 from Creative biogene
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shRNA set against Human DNAJA1(NM_001539.2) - SHH009353 from Creative biogene
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DNAJA1 shRNA Plasmid (Human) - CHH2257 from Cohesion Biosciences
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DNAJA1 miRNA 3'UTR clone - MiUTR1H-02904 from Creative biogene
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