VPS45

Gene Symbol VPS45
Entrez Gene 11311
Alt Symbol H1, H1VPS45, SCN5, VPS45A, VPS45B, VPS54A, VSP45, VSP45A
Species Human
Gene Type protein-coding
Description vacuolar protein sorting 45 homolog (S. cerevisiae)
Other Description hlVps45|leucocyte vacuolar protein sorting 45|vacuolar protein sorting 45A|vacuolar protein sorting-associated protein 45
Swissprots Q15715 D3DUZ9 Q5T4P6 Q9NRW7 Q9Y4Z6 F5H8K1 Q53FR8
Accessions CAI14265 CAI14266 EAW53584 EAW53585 Q9NRW7 AF165513 AAF86643 AJ133421 CAB40417 AK023170 BAB14443 AK223214 BAD96934 AK293555 BAH11533 AK295529 BAH12096 AK298786 BAH12870 AK298835 BAH12880 AK301994 BAH13603 AK307949 AY453586 AAS49034 BC012932 AAH12932 BC028382 AAH28382 BG176899 CR743023 DA122826 DA374440 JF432313 ADZ15530 U35246 AAC50931 XR_921733 XR_921734 XR_921735 NM_001279353 NP_001266282 NM_001279354 NP_001266283 NM_001279355 NP_001266284 NM_007259 NP_009190 NR_103998
Function May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network.
Subcellular Location Golgi apparatus membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Endosome membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Note=Associated with Golgi/endosomal vesicles and the trans-Golgi network. {ECO:0000250}.
Tissue Specificity Ubiquitous. Expression was highest in testis, heart and brain, intermediate in kidney, spleen, prostate, ovary, small intestine and thymus and low in lung, skeletal muscle, placenta, colon, pancreas, peripheral blood leukocytes and liver.
Top Pathways Endocytosis

Anti-VPS45A antibody - ab168515 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-VPS45A antibody (ab168515) at 1 µg/mlLane 1 : VPS45A transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateLysates/proteins at 15 µl per lane.

Anti-VPS45A antibody - ab129298 from Abcam

Prices $407.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab129208, at 3.75&microg/ml, staining VPS45A in Formalin-fixed, Paraffin-embedded Human spleen tissue by Immunohistochemistry.

Anti-VPS45A antibody - ab52013 from Abcam

Prices $370.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-VPS45A antibody (ab52013) at 0.3 µg/ml + Human Peripheral Blood Mononucleocyte lysate (35µg protein in RIPA buffer).

Anti-VPS45A antibody - Carboxyterminal end - ab56157 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-VPS45 Antibody (C-Terminus) - LS-C47065 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-VPS45 Antibody (Internal) - LS-C95247 from LifeSpan Bioscience

Prices $325.00
Sizes 500 µg
Host Rabbit
Clonality Polyclonal

Anti-VPS45 Antibody (aa461-489, AP) - LS-C242611 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS45 Antibody (aa461-489, APC) - LS-C242612 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS45 Antibody (aa427-476) - LS-C135275 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
VPS45 antibody LS-C135275 Western blot of Mouse Heart lysate. Antibody concentration 1 ug/ml.

Anti-VPS45 Antibody (aa461-489, Biotin) - LS-C242614 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS45 Antibody (aa461-489, PE) - LS-C242618 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS45 Antibody (aa461-489, FITC) - LS-C242615 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS45 Antibody (aa461-489, Azide-free, HRP) - LS-C247227 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS45 Antibody (aa460-489) - LS-C326293 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS45 Antibody (aa558-570) - LS-C55680 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.3 ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-VPS45 Antibody (aa468-517) - LS-C170223 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-VPS45 Antibody (aa461-489) - LS-C160540 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
VPS45 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The VPS45 antibody detected the VPS45 protein (arrow).

Anti-VPS45 Antibody (aa294-307) IHC-plus™ - LS-B6464 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-VPS45 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6464 concentration 2.5 ug/ml.

VPS45 Antibody - NBP1-81642 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VPS45 Antibody [NBP1-81642] - Analysis in human cell line RT-4.

VPS45 Antibody - NBP1-81641 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VPS45 Antibody [NBP1-81641] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

VPS45 Antibody - NB100-2431 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: VPS45 Antibody [NB100-2431] - (0.03ug/ml) staining of human placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

VPS45 Antibody - NB100-41372 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: VPS45 Antibody [NB100-41372] - (0.3ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

VPS45 Antibody - H00011311-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: VPS45 Antibody [H00011311-B01P] - Analysis of VPS45 expression in transfected 293T cell line by VPS45 polyclonal antibody. Lane 1: VPS45A transfected lysate(62.7 KDa). Lane 2: Non-transfected lysate.

Anti-VPS45 antibody produced in rabbit - HPA027425 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-VPS45: <B>Cat. No. HPA027425</B>: Immunohistochemical staining of human fallopian tube shows strong membranous positivity in ciliated glandular cells.

Anti-VPS45 (266-280) antibody produced in rabbit - SAB1101450 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-VPS45 (266-280) : <B>Cat. No. SAB1101450</B>: Immunofluorescence of HUVEC cells using VPS45 (266-280) , Cat. No. SAB1101450 (red) at a 1:50 dilution, taken at 40x magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
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