ALDH1A1

Gene Symbol ALDH1A1
Entrez Gene 216
Alt Symbol ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1
Species Human
Gene Type protein-coding
Description aldehyde dehydrogenase 1 family, member A1
Other Description ALDH class 1|ALHDII|RALDH 1|acetaldehyde dehydrogenase 1|aldehyde dehydrogenase 1, soluble|aldehyde dehydrogenase, liver cytosolic|epididymis luminal protein 12|epididymis luminal protein 9|epididymis secretory sperm binding protein Li 53e|retinal dehydrogenase 1|retinaldehyde dehydrogenase 1
Swissprots O00768 P00352 Q5SYR1
Accessions AAA51692 AAD13925 AAD14823 AAP88039 CBH19844 EAW62543 EAW62544 P00352 AB209821 BAD93058 AF003341 AAC51652 AI074764 AK000118 AK026641 AK293176 BAG56719 AK302168 BAG63535 AY390731 AAR92229 BC001505 AAH01505 BG773210 BT006921 AAP35567 DC398835 DQ890669 ABM81595 DQ893850 ABM84776 EU668318 ACF94471 EU794589 ACJ13643 FJ224286 ACI45978 K03000 AAA51695 M26761 AAA35518 NM_000689 NP_000680
Function Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm.
Top Pathways Retinol metabolism

ALDH1A1 (D4R9V) Rabbit mAb - 12035 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4R9V
Western blot analysis of extracts from Hep 3B and Hep G2 cells using ALDH1A1 (D4R9V) Rabbit mAb.

ALDH1/2 (H-85) - sc-50385 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ALDH1/2 (H-85): sc-50385. Western blot analysis of ALDH1/2 expression in Hep G2 whole cell lysate (A) and mouse liver (B) and rat liver (C) tissue extracts.

ALDH1A1 (A-6) - sc-398578 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ALDH1A1 (A-6): sc-398578. Western blot analysis of ALDH1A1 expression in non-transfected 293T: sc-117752 (A), human ALDH1A1 transfected 293T: sc-174232 (B), Hep G2 (C) and A549 (D) whole cell lysates and human liver tissue extract (E).

ALDH1A1 (B-5) - sc-374149 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ALDH1A1 (B-5): sc-374149. Western blot analysis of ALDH1A1 expression in Hep G2 (A) and K-562 (B) whole cell lysates.

ALDH1A1 (H-4) - sc-374076 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ALDH1A1 (H-4): sc-374076. Western blot analysis of ALDH1A1 expression in Hep G2 whole cell lysate.

ALDH1A1 (L-15) - sc-22589 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ALDH1A1 (L-15): sc-22589. Western blot analysis of ALDH1A1 expression in K-562 whole cell lysate.

ALDH1A1 (N-19) - sc-22588 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ALDH1A1 (N-19): sc-22588. Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.

Anti-ALDH1A1 antibody - ab131068 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ALDH1A1 antibody - ab191229 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ALDH1A1 antibody (ab191229) at 1/1000 dilutionLane 1 : A549 cell lysateLane 2 : Rat liver tissue lysateLysates/proteins at 35 µg per lane.SecondaryGoat anti-rabbit IgG H&L(HRP) at 1/5000 dilutiondeveloped using the ECL technique

Anti-ALDH1A1 antibody - ab89431 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-ALDH1A1 antibody (ab89431) at 1 µg/ml + human liver lysate at 50 µg

Anti-ALDH1A1 antibody [1G6] - ab124236 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1G6
ab124236 at 5µg/ml staining ALDH1A1 in formalin fixed, paraffin embedded Human colon tissue by Immunohistochemistry.

Anti-ALDH1A1 antibody [5A11] - ab105920 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5A11
Overlay histogram showing HepG2 cells stained with ab105920 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105920, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-ALDH1A1 antibody [EP1932Y] - ab134188 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1932Y
Anti-ALDH1A1 antibody [EP1932Y] (ab134188) at 1/1000 dilution + HepG2 cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-ALDH1A1 antibody [EP1933Y] - ab52492 from Abcam

Prices $403.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1933Y
Anti-ALDH1A1 antibody [EP1933Y] (ab52492) at 1/2000 dilution + HepG2 cell lysate at 10 µgSecondaryHRP labeled Goat anti-rabbit at 1/2000 dilution

Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 488) - ab195254 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1933Y
ab195254 staining ALDH1A1 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195254 at a working dilution of 1 in 100 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 647) - ab195255 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1933Y
ab195255 staining ALDH1A1 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195255 at a working dilution of 1 in 50 (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-ALDH1A1 antibody [EP1933Y] (HRP) - ab195517 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1933Y
Anti-ALDH1A1 antibody [EP1933Y] (HRP) (ab195517) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-ALDH1A1 antibody [mAbcam61293] - ab61293 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam61293
All lanes : Anti-ALDH1A1 antibody [mAbcam61293] (ab61293) at 10 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-ALDH1A1 antibody [mAbcam61293] (FITC) - ab151613 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam61293

Anti-ALDH1A1 antibody - C-terminal - ab151498 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-ALDH1A1 antibody - C-terminal (ab151498) at 1/10000 dilution + A431 whole cell lysate at 30 µg

Anti-ALDH1A1 antibody - N-terminal - ab190671 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-ALDH1A1 antibody - N-terminal (ab190671) at 1/500 dilution + Mouse lung extract at 30 µg

Anti-ALDH1A1 antibody - Neuronal Marker - ab23375 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab23375 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab23375, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Anti-ALDH1A1 / ALDH1 Antibody (aa2-501) - LS-C126706 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-ALDH1A1 / ALDH1 Antibody (aa488-501) - LS-C54480 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody staining (0.03 ug/ml) of human liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Anti-ALDH1A1 / ALDH1 Antibody (clone 152CT1.2.2, Azide-free, HRP) - LS-C250112 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 152CT1.2.2
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