CDK6

Gene Symbol CDK6
Entrez Gene 1021
Alt Symbol MCPH12, PLSTIRE
Species Human
Gene Type protein-coding
Description cyclin-dependent kinase 6
Other Description cell division protein kinase 6|serine/threonine-protein kinase PLSTIRE
Swissprots Q00534 A4D1G0
Accessions AAM76970 EAL24146 EAW76827 EAW76828 EAW76829 Q00534 AA312213 AB073601 AI023175 AI367890 AI400582 AI738486 AI935072 AK000660 AK055922 AK123134 AK172791 AK313491 BAG36273 AV726767 AW665776 BC012914 BC027989 BC052264 AAH52264 BC063591 BC065026 BE893003 BG166127 BM542578 BM542629 BM542736 BM905472 BM978533 BQ048881 BQ213893 BQ231384 BQ773638 BU159055 X66365 CAA47008 XM_006715835 XP_006715898 XM_006715836 XP_006715899 XM_011515731 XP_011514033 NM_001145306 NP_001138778 NM_001259 NP_001250
Function Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents my
Subcellular Location Cytoplasm. Nucleus. Cell projection, ruffle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:23918663}. Note=Localized to the ruffling edge of spreading fibroblasts. Kinase activity only in nucleus. Localized to the cytosol of neurons and showed prominent staining around either side of the nucleus (By similarity). Present in the cytosol and in the nucleus in interphase cells and at the centrosome during mitosis from prophase to telophase (PubMed:23918663). {ECO:0000250|UniProtKB:Q64261, ECO:0000269|PubMed:23918663}.
Tissue Specificity Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells. {ECO:0000269|PubMed:20668294, ECO:0000269|PubMed:8114739}.
Top Pathways Cell cycle, Glioma, Pancreatic cancer, Measles, Non-small cell lung cancer

CDK6 (D4S8S) Rabbit mAb - 13331 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4S8S
Western blot analysis of extracts from HeLa, Jurkat, and COS-7 cells using CDK6 (D4S8S) Rabbit mAb.

CDK6 (DCS83) Mouse mAb - 3136 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (20 western blots)
Host Mouse
Clonality Monoclonal
Clone DCS83
Western blot analysis of extracts from HeLa, IM-CD-3 and C6 cells, using CDK6 (DCS83) Mouse mAb.

Cdk6 (F-7) - sc-390493 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk6 (F-7): sc-390493. Western blot analysis of Cdk6 expression in Jurkat (A) and K-562 (B) whole cell lysates.

Cdk6 (C-21) - sc-177 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cdk6 (C-21): sc-177. Western blot analysis of Cdk6 expression in K-562 (A) and Jurkat (B) whole cell lysates.

Cdk6 (B-10) - sc-7961 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk6 (B-10): sc-7961. Western blot analysis of Cdk6 expression in K-562 (A) and Jurkat (B) whole cell lysates.

Cdk6 (H-11) - sc-271364 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk6 (H-11): sc-271364. Western blot analysis of Cdk6 expression in Jurkat whole cell lysate.

Cdk6 (DCS-90) - sc-56282 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk6 (DCS-90): sc-56282. Western blot analysis of Cdk6 expression in K-562 (A) and Jurkat (B) whole cell lysates.

Cdk6 (H-230) - sc-7181 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of Cdk6 expression in phorbol ester-induced Jurkat nuclear extract. Antibodies tested include Cdk6 (H-230): sc-7181 (A) and Cdk6 (H-96): sc-7180 (B).

Cdk6 (DCS-83) - sc-53638 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk6 (DCS-83): sc-53638. Western blot analysis of Cdk6 expression in K-562 (A) and Jurkat (B) whole cell lysates and phorbol treated Jurkat (C), phorbol treated K-562 (D) and Jurkat (E) nuclear extracts.

Cdk6 (H-96) - sc-7180 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cdk6 (H-96): sc-7180. Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing nuclear and cytoplasmic staining of hematopoietic cells.

Cdk6 (N-18) - sc-32501 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cdk6 (N-18): sc-32501. Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing nuclear and cytoplasmic staining of hematopoietic cells.

Cdk6 (SPM383) - sc-56362 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk6 (SPM383): sc-56362. Western blot analysis of Cdk6 expression in Jurkat whole cell lysate.

p-Cdk6 (Tyr 24) - sc-293097 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Cdk6 (Tyr 24): sc-293097. Western blot analysis of Cdk6 phosphorylation expression in untreated (A) and serum-starved treated (B) 293 whole cell lysates.

Anti-Cdk6 (phospho Y13) antibody - ab131439 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cdk6 (phospho Y13) antibody (ab131439) at 1/500 dilutionLane 1 : 293 cell extract untreatedLane 2 : 293 cell extract treated with Hydroxyurea

Anti-Cdk6 (phospho Y13) antibody - ab194871 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cdk6 (phospho Y13) antibody (ab194871) at 1/500 dilutionLane 1 : Untreated 293 cell lysateLane 2 : 293 cell lysate treated with HU

Anti-Cdk6 (phospho Y24) antibody - ab131469 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cdk6 (phospho Y24) antibody (ab131469) at 1/500 dilutionLane 1 : Extract from 293 cells; untreatedLane 2 : Extract from 293 cells; treated with starvation

Anti-Cdk6 antibody - ab54576 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Cdk6 antibody (ab54576) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.

Anti-Cdk6 antibody - ab131532 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of methanol-fixed HeLa cells labelling Cdk6 with ab131532 at 1/100 dilution.

Anti-Cdk6 antibody - ab157519 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Cdk6 antibody (ab157519) at 1/1000 dilution + C6 (rat glioma) cell lysate

Anti-Cdk6 antibody - ab76671 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Cdk6 antibody (ab76671) + HL60 at 35 µg

Anti-Cdk6 antibody [DCS-90] - ab6317 from Abcam

Host Mouse
Clonality Monoclonal
Clone DCS-90

Anti-Cdk6 antibody [EPR4515] - ab124821 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4515
Overlay histogram showing HeLa cells stained with ab124821 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124821, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Cdk6 antibody [EPR4515] (Alexa Fluor® 488) - ab198944 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4515
Overlay histogram showing HeLa cells stained with ab198944 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab198944, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Cdk6 antibody [EPR4515] (Alexa Fluor® 647) - ab198946 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4515
ab198946 staining Cdk6 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198946 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

Anti-Cdk6 antibody [EPR4515] (HRP) - ab198945 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4515
All lanes : Anti-Cdk6 antibody [EPR4515] (HRP) (ab198945) at 1/5000 dilutionLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.
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