COIL

Gene Symbol COIL
Entrez Gene 8161
Alt Symbol CLN80, p80-coilin
Species Human
Gene Type protein-coding
Description coilin
Other Description coilin p80|p80
Swissprots B2R931 P38432
Accessions EAW94522 P38432 AK313616 BAG36378 AW021880 BC010385 AAH10385 DQ892927 ABM83853 DQ896176 ABM87175 M58411 AAA36412 U06632 AAB81550 NM_004645 NP_004636
Function Component of nuclear coiled bodies, also known as Cajal bodies or CBs, which are involved in the modification and assembly of nucleoplasmic snRNPs. {ECO:0000269|PubMed:7679389}.
Subcellular Location Nucleus {ECO:0000269|PubMed:7679389}. Nucleus, Cajal body {ECO:0000269|PubMed:7679389}.
Tissue Specificity Found in all the cell types examined.

Coilin (D2L3J) XP ® Rabbit mAb - 14168 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2L3J
Western blot analysis of extracts from IMR-32, HeLa, and COS-7 cells using Coilin (D2L3J) XP ® Rabbit mAb.

coilin (B-7) - sc-55595 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
coilin (B-7): sc-55595. Western blot analysis of coilin expression in MOLT-4 (A) and Jurkat (B) nuclear extracts.

coilin (L-20) - sc-26110 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

coilin (H-300) - sc-32860 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
coilin (H-300): sc-32860. Western blot analysis of coilin expression in non-transfected: sc-117752 (A) and mouse coilin transfected: sc-125155 (B) 293T whole cell lysates and MOLT-4 nuclear extract (C).

coilin (Pdelta) - sc-56298 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
coilin (Pdelta): sc-56298. Western blot analysis of human recombinant coilin fusion protein.

coilin (F-7) - sc-55594 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
coilin (F-7): sc-55594. Western blot analysis of coilin expression in MOLT-4 (A) and Jurkat (B) nuclear extracts.

coilin (N-18) - sc-26109 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

coilin (56) - sc-135871 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/500 µl
Host Mouse
Clonality Monoclonal
coilin (56): sc-135871. Western blot analysis of coilin expression in K-562 whole cell lysate.

Anti-Coilin antibody - ab74739 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Coilin antibody (ab74739) at 1 µg/ml + Jurkat cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-Coilin antibody - ab167253 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-Coilin antibody (ab167253) at 1 µgLane 1 : Coilin transfected 293T cell lysateLane 2 : Non-transfected lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-Coilin antibody - ab124067 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Coilin antibody (ab124067) at 1/100 dilution + MCF7 cell line lysa at 35 µg

Anti-Coilin antibody [1H10] - ab119713 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1H10
All lanes : Anti-Coilin antibody [1H10] (ab119713) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : SK-N-MC cell lysateLane 3 : K562 cell lysateLane 4 : A431 cell lysateLane 5 : HepG2 cell lysateLane 6 : SK-BR3 cell lysateLane 7 : Ramos cell lysate

Anti-Coilin antibody [EPR7843(N)] - N-terminal - ab177466 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7843(N)
Flow cytometric analysis of permeabilized Jurkat cells labeling Coilin (red) with ab177466 at 1/100 dilution or a rabbit IgG (negative) (green).

Anti-Coilin antibody [IH10] - ab87913 from Abcam

Prices $392.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone IH10
IHC image of Coilin staining in human testis formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab87913, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97250, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-Coilin antibody [IH10] (Alexa Fluor® 488) - ab197531 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone IH10
ab197531 staining Coilin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197531 at 1/1000 dilution (shown in green) and ab6046 (Rabbit polyclonal to beta Tubulin) at 1µg/ml. This was followed by an incubation at room temperature for 1h with ab150088, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594), pre-adsorbed, at 1µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Coilin antibody [IH10] (Alexa Fluor® 647) - ab196714 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone IH10
ab196714 staining Coilin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196714 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

Anti-Coilin antibody [IH10] (FITC) - ab150278 from Abcam

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone IH10

Anti-Coilin antibody [IH10] (HRP) - ab196715 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone IH10
All lanes : Anti-Coilin antibody [IH10] (HRP) (ab196715) at 1/2000 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Coilin antibody [IH10] - BSA and Azide free - ab175346 from Abcam

Prices $373.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone IH10

Anti-Coilin antibody [Pdelta] - ab11822 from Abcam

Prices $384.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone Pdelta
ICC/IF image of ab11822 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11822, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Coilin antibody - C-terminal - ab176684 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Coilin in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176684 at 6 µg/mg lysate for IP and at 1µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds. 

Anti-COIL / Coilin Antibody - LS-C76887 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-COIL / Coilin Antibody (C-Terminus) - LS-C125379 from LifeSpan Bioscience

Prices $530.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal

Anti-COIL / Coilin Antibody - LS-C137362 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-COIL / Coilin Antibody (clone GF1) - LS-C189897 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone GF1
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