MED16

Gene Symbol MED16
Entrez Gene 10025
Alt Symbol DRIP92, THRAP5, TRAP95
Species Human
Gene Type protein-coding
Description mediator complex subunit 16
Other Description mediator of RNA polymerase II transcription subunit 16|thyroid hormone receptor-associated protein 5|thyroid hormone receptor-associated protein complex 95 kDa component|thyroid hormone receptor-associated protein, 95-kD subunit|vitamin D3 receptor-interacting protein complex 92 kDa component
Swissprots Q9Y652 Q96AD4 Q96I35 Q9Y2X0 Q6PJT2
Accessions EAW69586 EAW69587 Q9Y2X0 AF106934 AAD31087 AF121228 AAD30032 AL137732 AU098434 BC004554 AAH04554 BC007853 AAH07853 BC011841 AAH11841 BC017282 AAH17282 BI461485 BQ015734 CB050542 DQ893678 ABM84604 DQ895933 ABM86859 EU392448 ACB88881 EU392449 ACB88882 EU392450 ACB88883 EU392451 ACB88884 EU392452 ACB88885 EU392453 ACB88886 EU392454 ACB88887 EU392455 ACB88888 EU392456 ACB88889 EU392457 ACB88890 EU392458 ACB88891 EU392459 ACB88892 EU392460 ACB88893 EU392461 ACB88894 EU392462 ACB88895 EU392463 ACB88896 EU392464 ACB88897 EU392465 ACB88898 EU392466 ACB88899 EU392467 ACB88900 EU392468 ACB88901 EU392469 ACB88902 EU392470 ACB88903 EU392471 ACB88904 EU392472 ACB88905 EU392473 ACB88906 EU392474 ACB88907 EU392475 ACB88908 EU392476 ACB88909 EU392477 ACB88910 EU392478 ACB88911 EU392479 ACB88912 EU392480 ACB88913 EU392481 ACB88914 EU392482 EU392483 EU392484 EU392485 EU392486 EU392487 EU392488 EU392489 EU392490 EU392491 EU392492 EU392493 EU392494 EU392495 EU392496 EU392497 EU392498 XM_011527604 XP_011525906 XR_936134 NM_005481 NP_005472
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. {ECO:0000269|PubMed:10198638, ECO:0000269|PubMed:10235266}.
Subcellular Location Nucleus {ECO:0000305}.

TRAP95 (C-19) - sc-5363 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TRAP95 (C-19): sc-5363. Western blot analysis of TRAP95 expression in HeLa nuclear extract (A) and Jurkat whole cell lysate (B).

TRAP95 (H-300) - sc-292427 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

TRAP95 (L-15) - sc-5365 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TRAP95 (N-16) - sc-5366 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TRAP95 (E-24) - sc-134109 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
TRAP95 (E-24): sc-134109. Western blot analysis of human TRAP95 transfected 293T whole cell lysate.

TRAP95 (F83) - sc-130439 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
TRAP95 (F83): sc-130439. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

Anti-THRAP5 antibody - ab28520 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab28520, at 4.0-8.0ug/ml , staining human THRAP5 in myocardial cells by Immunohistochemistry, Paraffin embedded tissue.

Anti-THRAP5 antibody - ab126960 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-THRAP5 antibody (ab126960) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : HeLa whole cell lysateLane 3 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-THRAP5 antibody - ab56939 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab56939 THRAP5 antibody at 1ug/ml.

Anti-THRAP5 antibody - N-terminal - ab174865 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-THRAP5 antibody - N-terminal (ab174865) at 1/1000 dilutionLane 1 : Mouse thymus total cell extractLane 2 : HeLa total cell extractLane 3 : Jurkat total cell extract

Anti-MED16 / THRAP5 Antibody (aa252-281, AP) - LS-C246492 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED16 / THRAP5 Antibody (aa252-281, APC) - LS-C246493 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED16 / THRAP5 Antibody (aa252-281, Biotin) - LS-C246494 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED16 / THRAP5 Antibody (aa252-281, FITC) - LS-C246495 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED16 / THRAP5 Antibody (aa252-281, PE) - LS-C246498 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED16 / THRAP5 Antibody - LS-C155407 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 5% SDS PAGE MED16 / THRAP5 antibody diluted at 1:1000

Anti-MED16 / THRAP5 Antibody (aa171-220) - LS-C79965 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
721_B cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 8%.

Anti-MED16 / THRAP5 Antibody (aa252-281, Azide-free, HRP) - LS-C248786 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED16 / THRAP5 Antibody (aa1-111, Azide-free) - LS-C328940 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-MED16 / THRAP5 Antibody (aa252-281) - LS-C322935 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED16 / THRAP5 Antibody (aa686-735) - LS-C82004 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-MED16 / THRAP5 Antibody (aa749-798) - LS-C31444 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
MED16 / THRAP5 antibody LS-C31444 was used in IHC to stain formalin-fixed, paraffin-embedded human heart.

Anti-MED16 / THRAP5 Antibody (aa131-180) - LS-C205818 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MED16 / THRAP5 antibody Western Blot of 293T. Antibody dilution: 1 ug/ml.

Anti-MED16 / THRAP5 Antibody (aa253-281) - LS-C164116 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
MED16 Antibody western blot of 293 cell line lysates (35 ug/lane). The MED16 antibody detected the MED16 protein (arrow).

Anti-MED16 / THRAP5 Antibody (aa550-600) - LS-C290326 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human MED16 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MED16 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. MED16 was also immunoprecipitated by rabbit anti-MED16 antibody BL13259, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
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