ZNF282

Gene Symbol	ZNF282
Entrez Gene	8427
Alt Symbol	HUB1
Species	Human
Gene Type	protein-coding
Description	zinc finger protein 282
Other Description	HTLV-I U5 repressive element-binding protein 1\|HTLV-I U5RE-binding protein 1\|HUB-1
Swissprots	Q6DKK0 O43691 B4DRI5 Q9UDV7
Accessions	EAL24430 EAW80055 EAW80056 Q9UDV7 AK299280 BAG61297 AK316282 BAH14653 AY927504 AY927505 BC009542 BC031686 AAH31686 BC041704 AAH41704 BC055287 AAH55287 BC073805 AAH73805 BU731447 CV573257 D30612 BAA24380 XM_006716151 XP_006716214 XM_011516628 XP_011514930 NM_001303481 NP_001290410 NM_003575 NP_003566
Function	Binds to the U5 repressive element (U5RE) of the human T cell leukemia virus type I long terminal repeat. It recognizes the 5'-TCCACCCC-3' sequence as a core motif and exerts a strong repressive effect on HTLV-I LTR-mediated expression.
Subcellular Location	Nucleus {ECO:0000305}.
Tissue Specificity	Ubiquitous.

ZNF282 (D-13) - sc-132168 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

ZNF282 (D-13): sc-132168. Western blot analysis of ZNF282 expression in non-transfected: sc-117752 (A) and mouse ZNF282 transfected: sc-124786 (B) 293T whole cell lysates.

ZNF282 (N-13) - sc-132169 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

ZNF282 (T-13) - sc-132170 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

ZNF282 (T-13): sc-132170. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

ZNF282 (J-23) - sc-102202 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

ZNF282 (J-23): sc-102202. Western blot analysis of ZNF282 expression in transfected 293T whole cell lysate.

Anti-ZNF282 antibody - ab50665 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ZNF282 antibody (ab50665) at 1.25 µg/ml + 293T cell lysate. at 10 µgSecondaryHRP conjugated anti-Rabbit IgG (1:100,000)

Anti-ZNF282 antibody - ab127540 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ZNF282 antibody (ab127540) at 1/1000 dilution + Raji whole cell lysate at 30 µg

Anti-ZNF282 Antibody (N-Terminus) - LS-C31372 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ZNF282 Antibody (C-Terminus) - LS-C31374 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ZNF282 Antibody - LS-C155576 from LifeSpan Bioscience

Prices	$335.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE ZNF282 / HUB1 antibody diluted at 1:1000

ZNF282 Antibody - NBP1-81653 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence: ZNF282 Antibody [NBP1-81653] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

ZNF282 Antibody - NBP2-20985 from Novus Biologicals

Prices	$349.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: ZNF282 Antibody [NBP2-20985] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.

Anti-ZNF282 (AB2) antibody produced in rabbit - AV38361 from Sigma-Aldrich

Prices	$267.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Cell Type: transfected 293T (<B>Cat. No. AV38361</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 1.25 μg/mL in cell type transfected 293T

Anti-ZNF282 antibody produced in rabbit - HPA024374 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-ZNF282: <B>Cat. No. HPA024374</B>: Immunohistochemical staining of human hippocampus shows strong nuclear positivity in neuronal cells.

Anti-ZNF282 (AB1) antibody produced in rabbit - AV38360 from Sigma-Aldrich

Prices	$267.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Cell Type: transfected 293T (<B>Cat. No. AV38360</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 1.25 μg/mL in cell type transfected 293T

ZNF282 antibody - N-terminal region (ARP38360_T100) - ARP38360_T100 from Aviva Systems Biology

Prices	$229.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

ZNF282 antibody - C-terminal region (ARP38361_T100) - ARP38361_T100 from Aviva Systems Biology

Prices	$229.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

ZNF282 Antibody - 28-849 from ProSci

Prices	$250.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Antibody used in WB on Transfected 293T at 1.25 ug/ml.

ZNF282 Antibody - 28-850 from ProSci

Prices	$250.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

ZNF282 Antibody - GWB-750F13 from Genway Biotech

Prices	$268.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

ZNF282 Antibody - GWB-69C1EC from Genway Biotech

Prices	$268.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Yeast Hub1 Antibody - GWB-11F9B0 from Genway Biotech

Prices	$500.00
Sizes	500 μg
Host	Rabbit

Anti-ZNF282 (aa 46-263) polyclonal antibody - DPABH-23190 from Creative Diagnostics

Host	Rabbit
Clonality	Polyclonal

ZNF282 Polyclonal Antibody - PA5-31276 from Thermo Fisher Scientific

Prices	$349.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of ZNF282 using 30µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a ZNF282 polyclonal antibody (Product # PA5-31276) at a dilution of 1:1000.

ZNF282 - MBS274530 from MyBioSource

Prices	$360.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:1000

ZNF282-antibody-C-terminal-region-AI11402 - AI11402 from Abgent, a WuXi AppTec company

Prices	$232.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

WB Suggested Anti-ZNF282 Antibody Titration: 1.25ug/ml ELISA Titer: 1:312500 Positive Control: Transfected 293T

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