PSMF1

Gene Symbol	PSMF1
Entrez Gene	9491
Alt Symbol	PI31
Species	Human
Gene Type	protein-coding
Description	proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
Other Description	hPI31\|proteasome inhibitor PI31 subunit\|proteasome inhibitor hP131 subunit
Swissprots	D3DVW3 Q9H4I1 A0AVQ9 Q92530
Accessions	EAX10649 EAX10650 EAX10651 Q92530 AA885338 AF055032 AK098612 AK294134 BAG57462 AK300667 BAG62352 AK302164 BAG63531 BC029836 BC126127 BC126462 AAI26463 BI461198 BI830805 D88378 BAA13603 DA949012 DB028624 HQ258165 ADR82919 XM_005260873 XP_005260930 XM_005260874 XP_005260931 XM_006723664 XP_006723727 NM_006814 NP_006805 NM_178578 NP_848693 NM_178579
Function	Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28. {ECO:0000269\|PubMed:10764772}.
Subcellular Location	Cytoplasm {ECO:0000269\|PubMed:18495667}. Endoplasmic reticulum {ECO:0000269\|PubMed:18495667}.
Top Pathways	Proteasome

PI31 (26) - sc-136416 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

PI31 (26): sc-136416. Western blot analysis of PI31 expression in K562 whole cell lysate.

PI31 (S-20) - sc-85824 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

PI31 (S-20): sc-85824. Western blot analysis of PI31 expression in non-transfected: sc-117752 (A) and human PI31 transfected: sc-373110 (B) 293T whole cell lysates.

Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody - ab187200 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab187200) at 1/500 dilutionLane 1 : SW480 cell extractLane 2 : A549 cell extractLane 3 : K562 cell extractLane 4 : Mouse liver tissue extractLane 5 : Mouse testis tissue extract

Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody - ab140527 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab140527) at 0.1 µg/mlLane 1 : 293T whole cell lysate at 50 µgLane 2 : 293T whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µg

Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody - ab3893 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

ab3893 staining (1µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody - ab96845 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab96845) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-PSMF1 Antibody (C-Terminus) - LS-C55279 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Antibody staining (1 ug/ml) of Human Kidney lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Anti-PSMF1 Antibody (aa221-271) - LS-C290509 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human PSMF1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMF1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-PSMF1 Antibody (aa150-200) - LS-C290508 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PSMF1 Antibody - LS-C334133 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of various cell lines, using PSMF1 antibody.

Anti-PSMF1 Antibody (clone 2F9) - LS-C337834 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	2F9

Anti-PSMF1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMF1.

Anti-PSMF1 Antibody (aa209-271) - LS-C185652 from LifeSpan Bioscience

Prices	$335.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

PSMF1 antibody [C2C3], C-term detects PSMF1 protein at Cytoplasm and Nucleus by immunofluorescent analysis. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. PSMF1 protein stained by PSMF1 antibody [C2C3], C-term diluted at 1:500.

Anti-PSMF1 Antibody (clone 2D7) - LS-C174259 from LifeSpan Bioscience

Prices	$350.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	2D7

Anti-PSMF1 Antibody (aa110-159) - LS-C116992 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

PSMF1 antibody LS-C116992 Western blot of Jurkat lysate.

Anti-PSMF1 Antibody - LS-C341652 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

PSMF1 Antibody - NBP1-80910 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: PSMF1 Antibody [NBP1-80910] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

PSMF1 Antibody - NBP1-80911 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: PSMF1 Antibody [NBP1-80911] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

PSMF1 Antibody - NBP1-79738 from Novus Biologicals

Prices	$139.00, $329.00
Sizes	20 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: PSMF1 Antibody [NBP1-79738] - Titration: 0.2-1 ug/ml, Positive Control: Jurkat cell lysate.

PSMF1 Isoform 1 Antibody - NB100-1178 from Novus Biologicals

Prices	$299.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Western Blot: PSMF1 Isoform 1 Antibody [NB100-1178] - Analysis of PI31 / PSMF1 (Isoform 1) in Human Kidney lysate using NB100-1178 at 1 ug/ ml.

Anti-PSMF1 antibody produced in rabbit - HPA041300 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-PSMF1: <B>Cat. No. HPA041300</B>: Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Anti-PI31/PSMF1 (Isoform 1) antibody produced in goat - SAB2500788 from Sigma-Aldrich

Prices	$376.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-PI31 / PSMF1 (Isoform 1): <B>Cat. No. SAB2500788</B>: staining (1 μg/mL) of Human Kidney lysate (RIPA buffer, 30 μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-PSMF1 antibody produced in rabbit - HPA041122 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-PSMF1: <B>Cat. No. HPA041122</B>: Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.

PSMF1 Antibody - N-terminal region (ARP59166_P050) - ARP59166_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

PSMF1 antibody - middle region (ARP59167_P050) - ARP59167_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

PI31--PSMF1-Isoform-1-Antibody - AF1824a from Abgent, a WuXi AppTec company

Prices	$335.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

AF1824a staining (1 µg/ml) of Human Kidney lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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