MRAS

Gene Symbol MRAS
Entrez Gene 22808
Alt Symbol M-RAs, R-RAS3, RRAS3
Species Human
Gene Type protein-coding
Description muscle RAS oncogene homolog
Other Description muscle and microspikes RAS|ras-related protein M-Ras|ras-related protein R-Ras3
Swissprots Q86WX8 O14807 B4DIK0
Accessions EAW79068 EAW79069 EAW79070 EAW79071 O14807 AF022080 AAC52085 AF043938 AAD02287 AF493918 AAM12632 AI216578 AK056337 AK091633 AK123833 AK124954 BAG54117 AK295640 BAG58512 AK316071 BAH14442 BC017733 AAH17733 BC035939 BC047101 BC047690 BC156845 AAI56846 BI488489 BI546150 BT020057 AAV38860 CN356600 CR542067 CAG46864 DA235195 DA355883 XM_005247228 XP_005247285 XM_005247229 XP_005247286 NM_001085049 NP_001078518 NM_001252090 NP_001239019 NM_001252091 NP_001239020 NM_001252092 NP_001239021 NM_001252093 NP_001239022 NM_012219 NP_036351
Function May serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway. {ECO:0000269|PubMed:16630891}.
Subcellular Location Cell membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
Tissue Specificity Expression highly restricted to the brain and heart.
Top Pathways Tight junction, MAPK signaling pathway, HTLV-I infection, Rap1 signaling pathway, Proteoglycans in cancer

M-Ras (H-60) - sc-292844 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
M-Ras (H-60): sc-292844. Western blot analysis of M-Ras expression in Jurkat whole cell lysate.

M-Ras (K-16) - sc-12532 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
M-Ras (K-16): sc12532. Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.

M-Ras (N-19) - sc-8168 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-MRas antibody - ab26303 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MRas antibody (ab26303)Lane 1 : HEK 293T cell lysateLane 2 : MCF-7 cell lysateLane 3 : HeLa cell lysateSecondaryanti-rabbit HRP conjugateddeveloped using the ECL technique

Anti-MRas antibody - ab172409 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-MRas antibody (ab172409) at 1 µg/mlLane 1 : MRas transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-MRas antibody [3C4] - ab119064 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3C4
All lanes : Anti-MRas antibody [3C4] (ab119064) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY MRas cDNALysates/proteins at 5 µg per lane.

Anti-MRas antibody [EPR12457] - ab176570 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12457
All lanes : Anti-MRas antibody [EPR12457] (ab176570) at 1/1000 dilutionLane 1 : Fetal heart lysateLane 2 : Fetal brain lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Anti-MRAS Antibody (clone 3C4) - LS-C172952 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3C4
Anti-MRAS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MRAS.

Anti-MRAS Antibody - LS-C309057 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

M-Ras/R-Ras3 Antibody - AF3606 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal

M-Ras/R-Ras3 Antibody (3C4) - NBP2-00719 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3C4
Western Blot: MRAS Antibody (3C4) [NBP2-00719] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRAS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRAS.

M-Ras/R-Ras3 Antibody - NBP1-81039 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

M-Ras/R-Ras3 Antibody - H00022808-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: M-Ras/R-Ras3 Antibody [H00022808-B01P] - Analysis of MRAS expression in transfected 293T cell line by MRAS polyclonal antibody. Lane 1: MRAS transfected lysate(22.88 KDa). Lane 2: Non-transfected lysate.

M-Ras/R-Ras3 Antibody - H00022808-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: M-Ras/R-Ras3 Antibody [H00022808-B01] - analysis of MRAS expression in transfected 293T cell line by MRAS MaxPab polyclonal antibody (B01). Lane 1: MRAS transfected lysate (23.80 KDa). Lane 2: Non-transfected lysate.

M-RAS/Mras Polyclonal Antibody - bs-1882R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Paraformaldehyde-fixed, paraffin embedded rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MRas Polyclonal Antibody, Unconjugated (bs-1882R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Anti-MRAS antibody produced in mouse - SAB1407144 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-MRAS: <B>Cat. No. SAB1407144</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~22.9 kDa using MRAS transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-MRAS (C-term) antibody produced in rabbit - SAB1300903 from Sigma-Aldrich

Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-MRAS (C-term) Antibody: <B>Cat. No. SAB1300903</B>: MRAS in Jurkat cell line lysates with Anti-MRAS (C-term) Antibody at 4.0 μg/mL.

Anti-MRAS antibody produced in rabbit - HPA042728 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MRAS: <B>Cat. No. HPA042728</B>: Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Anti-MRAS (136-150) antibody produced in rabbit - SAB1101818 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-MRAS (136-150) (RB): <B>Cat. No. SAB1101818</B>: Immunofluorescence of HUVEC cells using Anti-MRAS (136-150) (RB) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Human/Rat M-Ras/R-Ras3 Antibody - AF3606 from R&D Systems

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Detection of Human and Rat M-Ras/R-Ras3 by Western Blot. Western blot shows lysates of L6 rat myoblast cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Rat M-Ras/R-Ras3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3606) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109 ). For additional reference, recombinant human R-Ras, TC21/R-Ras2, and M-Ras/R-Ras3 (2 ng/lane) were included. A specific band for M-Ras/R-Ras3 was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
Detection of Human and Rat M‑Ras/R‑Ras3 by Western Blot. Western blot shows lysates of L6 rat myoblast cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Rat M‑Ras/R‑Ras3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3606) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human R‑Ras, TC21/R-Ras2, and M‑Ras/R‑Ras3 (2 ng/lane) were included. A specific band for M‑Ras/R‑Ras3 was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MRAS antibody - 10R-4874 from Fitzgerald

Prices $455.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3C4

MRAS antibody - 70R-18588 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

M-RAS/Mras Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-1882R-A350 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

M-RAS/Mras Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-1882R-A488 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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