Gene Symbol | WDFY3 |
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Entrez Gene | 23001 |
Alt Symbol | ALFY, ZFYVE25 |
Species | Human |
Gene Type | protein-coding |
Description | WD repeat and FYVE domain containing 3 |
Other Description | WD repeat and FYVE domain-containing protein 3|autophagy-linked FYVE protein |
Swissprots | Q8N1T2 Q4W5K5 Q9Y2J7 Q8NAV6 Q96D33 Q8IZQ1 Q96N85 Q6P0Q5 Q96BS7 |
Accessions | AAY40903 EAX05952 EAX05953 EAX05954 EAX05955 EAX05956 Q8IZQ1 AB023210 BAA76837 AF131845 AF538685 AAN15137 AI393222 AI962188 AK055806 BAB71020 AK092019 AK092394 AK094910 BAC04455 BC013377 AAH13377 BC015214 AAH15214 BC065502 AAH65502 BC119633 AAI19634 BC148294 AAI48295 BC148298 AAI48299 BC150246 AAI50247 BE221984 BM676710 BQ003872 BX648077 BX648153 EF491804 EF491819 HQ447113 ADQ31600 XM_005262858 XP_005262915 XM_011531757 XP_011530059 XM_011531758 XP_011530060 XM_011531759 XP_011530061 XM_011531760 XP_011530062 XM_011531761 XP_011530063 XM_011531762 XP_011530064 XM_011531763 XP_011530065 XM_011531764 XP_011530066 XM_011531765 XP_011530067 XM_011531766 XP_011530068 XM_011531767 XP_011530069 NM_014991 NP_055806 NM_178583 NM_178585 |
Function | Required for selective autophagy (aggrephagy) but not for autophagic degradation of bulk cytospol in response to starvation. Involved in the formation and degradation of cytoplasmic polyubiquitin-containing bodies (p62 bodies, ALIS/aggresome-like induced structures). May play a role as adaptor or scaffolding protein by promoting the association of the E3-like ligase ATG12-ATG5-ATG16L and LC3 to ubiquitinated target substrate. The association with GABARAP is required for its recruitment to LC3B-positive p62 bodies suggesting a role in targeting certain p62 structures for clearance. Involved in midbody ring degradation. {ECO:0000269|PubMed:20168092, ECO:0000269|PubMed:20417604, ECO:0000269|PubMed:24128730}. |
Subcellular Location | Nucleus membrane. Cytoplasm. Nucleus, PML body. Membrane; Peripheral membrane protein; Cytoplasmic side. Note=Colocalizes with autophagic structures in starved cells. Shuttles between nucleus and cytoplasm; translocation from the nucleus to cytoplasmic polyubiquitin-containing bodies upon cellular stress is dependent on SQSTM1. |
Tissue Specificity | Ubiquitous. Expressed in osteoclast and their mononuclear precursors (at protein level). {ECO:0000269|PubMed:15292400, ECO:0000269|PubMed:20971078}. |
WDFY3 CRISPR/Cas9 KO Plasmid (h) - sc-406364 from Santa Cruz Biotechnology
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WDFY3 HDR Plasmid (h) - sc-406364-HDR from Santa Cruz Biotechnology
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WDFY3 Double Nickase Plasmid (h) - sc-406364-NIC from Santa Cruz Biotechnology
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WDFY3 Double Nickase Plasmid (h2) - sc-406364-NIC-2 from Santa Cruz Biotechnology
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WDFY3 CRISPR Activation Plasmid (h) - sc-406364-ACT from Santa Cruz Biotechnology
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WDFY3 CRISPR Activation Plasmid (h2) - sc-406364-ACT-2 from Santa Cruz Biotechnology
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WDFY3 Lentiviral Activation Particles (h) - sc-406364-LAC from Santa Cruz Biotechnology
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WDFY3 Lentiviral Activation Particles (h2) - sc-406364-LAC-2 from Santa Cruz Biotechnology
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WDFY3 siRNA (h) - sc-89191 from Santa Cruz Biotechnology
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WDFY3 shRNA Plasmid (h) - sc-89191-SH from Santa Cruz Biotechnology
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WDFY3 shRNA (h) Lentiviral Particles - sc-89191-V from Santa Cruz Biotechnology
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WDFY3 RNAi - H00023001-R01 from Novus Biologicals
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WDFY3 RNAi - H00023001-R02 from Novus Biologicals
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MISSION® esiRNA - EHU139991 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22800 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22800 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR07971 from Sigma-Aldrich
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WDFY3 siRNA (Human) - CRH7922 from Cohesion Biosciences
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WDFY3 RNAi - H00023001-R03 from Novus Biologicals
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WDFY3 - MBS8225627 from MyBioSource
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shRNA set against Human WDFY3 (NM_014991.4) - SHH445848 from Creative biogene
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Human WDFY3 siRNA - orb268424 from Biorbyt
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WDFY3 shRNA Plasmid (Human) - CHH7922 from Cohesion Biosciences
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WDFY3 miRNA 3'UTR clone - MiUTR4H-TG09826 from Creative biogene
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