WNK2

Gene Symbol	WNK2
Entrez Gene	65268
Alt Symbol	NY-CO-43, P/OKcl.13, PRKWNK2, SDCCAG43
Species	Human
Gene Type	protein-coding
Description	WNK lysine deficient protein kinase 2
Other Description	antigen NY-CO-43\|mitogen-activated protein kinase kinase kinase\|protein kinase lysine-deficient 2\|protein kinase with no lysine 2\|protein kinase, lysine deficient 2\|serine/threonine-protein kinase WNK2\|serologically defined colon cancer antigen 43
Swissprots	Q5VWF1 Q9Y3S1 Q9H3P4 Q9C0A3 Q8IY36 Q5VWF2
Accessions	EAW62857 EAW62858 EAW62859 EAW62860 Q9Y3S1 AB044546 BAB18648 AB051547 BAB21851 AF039702 AAC18051 AJ242724 CAB44308 AK000694 AK093910 AK096968 AK124314 AK310390 BC037965 AAH37965 HY151846 XM_005252137 XP_005252194 XM_005252140 XP_005252197 XM_005252141 XP_005252198 XM_005252144 XP_005252201 XM_011518926 XP_011517228 XM_011518927 XP_011517229 XM_011518928 XP_011517230 XM_011518929 XP_011517231 XM_011518930 XP_011517232 XM_011518931 XP_011517233 XM_011518932 XP_011517234 XM_011518933 XP_011517235 XM_011518934 XP_011517236 XM_011518935 XP_011517237 XM_011518936 XP_011517238 XM_011518937 XP_011517239 XM_011518938 XP_011517240 XM_011518939 XP_011517241 XM_011518940 XP_011517242 NM_001282394 NP_001269323 NM_006648 NP_006639
Function	Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity. {ECO:0000269\|PubMed:17667937, ECO:0000269\|PubMed:18593598, ECO:0000269\|PubMed:21733846}.
Subcellular Location	Cytoplasm. Cell membrane.
Tissue Specificity	Expressed in various cancer cell lines (at protein level). Predominantly expressed in heart, brain, skeletal muscle and colon. {ECO:0000269\|PubMed:11571656, ECO:0000269\|PubMed:17667937, ECO:0000269\|PubMed:21733846}.

WNK2 (H-210) - sc-98987 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

WNK2 (H-210): sc-98987. Western blot analysis of WNK2 expression in human heart tissue extract.

WNK2 (T-15) - sc-51251 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

WNK2 (W-14) - sc-51252 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

WNK2 (46.21) - sc-100452 from Santa Cruz Biotechnology

Prices	$295.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

WNK2 (46.21): sc-100452. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon tissue showing membrane and cytoplasmic localization.

Anti-WNK2 antibody - ab192397 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-WNK2 antibody (ab192397)Lane 1 : HeLa whole cell lysateLane 2 : Mouse sp2/0 whole cell lysateLane 3 : MCF7 whole cell lysate

Anti-WNK2 antibody - ab57691 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

WNK2 antibody (ab57691) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.

Anti-WNK2 antibody - ab133696 from Abcam

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-WNK2 antibody (ab133696) at 1 µg/ml + 721_B cell lysate at 10 µg

Anti-WNK2 antibody - ab28852 from Abcam

Prices	$242.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Top blot: anti Myc-Tag antibody; Bottom Blot: ab28852 (1/500)Lane 1 : Hek293 cell lysate Transfected with empty vectorLane 2 : Hek293 cell lysate Transfected with MYC-WNK1Lane 3 : Hek293 cell lysate Transfected with MYC-WNK2Lane 4 : Hek293 cell lysate Transfected with MYC-WNK3Lane 5 : Hek293 cell lysate Transfected with MYC-WKN4SecondaryGoat anti-rabbit IgG conjugated to HRP at 1/3000 dilutiondeveloped using the ECL technique

Anti-WNK2 Antibody (N-Terminus) - LS-C11067 from LifeSpan Bioscience

Prices	$415.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-WNK2 Antibody (aa2118-2218, Azide-free) - LS-C329109 from LifeSpan Bioscience

Prices	$485.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-WNK2 Antibody - LS-C340923 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-WNK2 Antibody (aa136-185) - LS-C151933 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-WNK2 Antibody (clone 2E10) - LS-C133809 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2E10

Immunoperoxidase of monoclonal antibody to WNK2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml].

WNK2 Antibody - 25910002 from Novus Biologicals

Prices	$369.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

WNK2 Antibody - NBP2-33875 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence: WNK2 Antibody [NBP2-33875] - Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.

WNK2 Antibody (2E10) - H00065268-M01 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2E10

WNK2 Antibody - NBP2-33876 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33876] - Staining of human fallopian tube shows cytoplasmic positivity in glandular cells.

Anti-WNK2 antibody produced in rabbit - HPA015555 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-WNK2: <B>Cat. No. HPA015555</B>: Immunohistochemical staining of Human pancreas shows cytoplasmic positivity in exocrine glandular cells.

Anti-WNK2 antibody produced in rabbit - HPA016519 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-WNK2: <B>Cat. No. HPA016519</B>: Immunohistochemical staining of Human duodenum shows strong cytoplasmic positivity in glandular cells.

Monoclonal Anti-WNK2 antibody produced in mouse - WH0065268M1 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2E10

<B>Immunoblotting</B><BR/>Monoclonal Anti-WNK2, <B>Cat. No. WH0065268M1</B> used at the antibody concentration: 1 μg/mL. Specific band of ~37.11 kDa using immunogen protein lysate.

Monoclonal Anti-WNK2, (C-terminal) antibody produced in mouse - SAB1405259 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2A2

<B>Immunoblotting</B><BR/>Monoclonal Anti-WNK2: <B>Cat. No. SAB1405259</B>: Antibody concentration: 1 μg/mL. Specific band of ~37.1 kDa using immunogen protein lysate.

WNK2 Antibody - N-terminal region (ARP65290_P050) - ARP65290_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

WNK2-Antibody-monoclonal-M01 - AT4541a from Abgent, a WuXi AppTec company

Prices	$315.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2E11

Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .

WNK2 Antibody - abx008052 from Abbexa

Host	Rabbit
Clonality	Polyclonal

WNK2 antibody - H00065268-M01 from Acris Antibodies

Prices	$360.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2E10

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