PRPF19

Gene Symbol PRPF19
Entrez Gene 27339
Alt Symbol NMP200, PRP19, PSO4, SNEV, UBOX4, hPSO4
Species Human
Gene Type protein-coding
Description pre-mRNA processing factor 19
Other Description PRP19/PSO4 homolog|PRP19/PSO4 pre-mRNA processing factor 19 homolog|nuclear matrix protein 200|nuclear matrix protein NMP200 related to splicing factor PRP19|pre-mRNA-processing factor 19|psoralen 4|senescence evasion factor
Swissprots Q9UMS4
Accessions EAW73904 EAW73905 Q9UMS4 AF086469 AJ131186 CAB51857 AL555169 AW673738 BC008719 AAH08719 BC018665 AAH18665 BC018698 AAH18698 DQ893003 ABM83929 DQ896249 ABM87248 NM_014502 NP_055317
Function Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex (PubMed:20595234). Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries (PubMed:21536736). The XAB2 complex, which contains PRPF19, is also involved in pre- mRNA splicing, transcription and transcription-coupled repair (PubMed:17981804). Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/D
Subcellular Location Nucleus {ECO:0000269|PubMed:11082287, ECO:0000269|PubMed:12429849, ECO:0000269|PubMed:19188445, ECO:0000269|PubMed:20176811}. Nucleus, nucleoplasm {ECO:0000269|PubMed:11082287}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:11082287}. Cytoplasm {ECO:0000269|PubMed:11435423}. Lipid droplet {ECO:0000250|UniProtKB:Q99KP6}. Note=Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin. Recruited on damaged DNA at sites of double-strand break. {ECO:0000269|PubMed:11082287, ECO:0000269|PubMed:18263876}.
Tissue Specificity Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines. {ECO:0000269|PubMed:10404385, ECO:0000269|PubMed:11082287, ECO:0000269|PubMed:11435423, ECO:0000269|PubMed:12960389, ECO:0000269|PubMed:16388800}.
Top Pathways Ubiquitin mediated proteolysis, Spliceosome

PRP19 (G-7) - sc-514338 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PRP19 (G-7): sc-514338. Western blot analysis of PRP19 expression in HeLa nuclear extract (A) and Jurkat (B) and A-431 (C) whole cell lysates.

PRP19 (C-18) - sc-47923 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PRP19 (C-18): sc-47923. Western blot analysis of PRP19 expression in HeLa nuclear extract (A) and HEK293 (B), HeLa (C), Jurkat (D) and MCF7 (E) whole cell lysates.

PRP19 (N-15) - sc-47925 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PRP19 (36-K) - sc-101236 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
PRP19 (36-K): sc-101236. Western blot analysis of PRP19 expression in HeLa nuclear extract.

Anti-PRP19 antibody - ab104171 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PRP19 antibody (ab104171) at 1/100 dilution + MCF-7 cell lysate at 35 µg

Anti-PRP19 antibody - ab57275 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-PRP19 antibody [EPR7446] - ab126776 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7446
All lanes : Anti-PRP19 antibody [EPR7446] (ab126776) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : A431 cell lysateLane 3 : MCF7 cell lysateLane 4 : PC12 cell lysateLane 5 : NIH 3T3 cell lysateLane 6 : HeLa cell lysateLane 7 : K562 cell lysateLane 8 : U937 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-PRPF19 / PRP19 Antibody (C-Terminus) - LS-C193736 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Guinea Pig
Clonality Polyclonal

Anti-PRPF19 / PRP19 Antibody (aa10-40, AP) - LS-C267179 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PRPF19 / PRP19 Antibody (aa10-40, APC) - LS-C267181 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PRPF19 / PRP19 Antibody (aa10-40, Biotin) - LS-C267182 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PRPF19 / PRP19 Antibody (N-Terminus) - LS-C31991 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
HepG2 Cell Lysate

Anti-PRPF19 / PRP19 Antibody (aa10-40, FITC) - LS-C267183 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PRPF19 / PRP19 Antibody (aa10-40, Azide-free, HRP) - LS-C267186 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PRPF19 / PRP19 Antibody (aa151-200) - LS-C200533 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of the lysates from HT-29 cells using PRPF19 antibody.

Anti-PRPF19 / PRP19 Antibody (aa10-40, PE) - LS-C267187 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PRPF19 / PRP19 Antibody (aa151-200) - LS-C31992 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
PRPF19 antibody LS-C31992 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.

Anti-PRPF19 / PRP19 Antibody (aa1-91, Azide-free) - LS-C328398 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-PRPF19 / PRP19 Antibody (aa10-40) - LS-C330021 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PRPF19 / PRP19 Antibody - LS-C334892 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of SW620 cell line, using PRPF19 antibody.

Anti-PRPF19 / PRP19 Antibody (C-Terminus) - LS-C287030 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of human PRP19/PSO4 by Western Blot. Samples: Whole cell lysate (40 ug for WB) from Jurkat cells. Antibody: Affinity purified rabbit anti-PRP19/PSO antibody used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 2 minutes.

Anti-PRPF19 / PRP19 Antibody (aa171-220) IHC-plus™ - LS-B9675 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PRPF19 / PRP19 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9675 concentration 10 ug/ml.

Anti-PRPF19 / PRP19 Antibody (Internal, clone EPR7446) - LS-C152380 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7446
IHC of paraffin-embedded human colon carcinoma tissue using anti-PRP19 antibody.

Anti-PRPF19 / PRP19 Antibody - LS-C332559 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using PRPF19 antibody.

Anti-PRPF19 / PRP19 Antibody (aa25-75) - LS-C286391 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse PRP19/PSO4 by Immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-PRP19/PSO4 used at a dilution of 1:250.
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