WIPF2

Gene Symbol WIPF2
Entrez Gene 147179
Alt Symbol WICH, WIRE
Species Human
Gene Type protein-coding
Description WAS/WASL interacting protein family, member 2
Other Description WAS/WASL-interacting protein family member 2|WASP-binding protein|WASP-interacting protein-related protein|WIP- and CR16-homologous protein|WIP-related protein
Swissprots Q8TF74 Q8TE44 Q71RE1 Q658J8 A8K0L3
Accessions EAW60648 EAW60649 Q8TF74 AB043786 BAB85113 AF370396 AAQ15232 AJ431177 CAD24007 AK026913 AK055025 AK055057 BAG51456 AK289578 BAF82267 AK289779 BAF82468 AK290301 BAF82990 AK311544 AK315836 BAF98727 AL834239 CAH56360 AU138315 BC019706 BC025965 BC030561 BC043408 BC047435 BC064504 BC065551 AAH65551 CB240594 CN404607 DQ586635 U90911 XM_005257083 XP_005257140 XM_005257084 XP_005257141 XM_011524412 XP_011522714 XM_011524413 XP_011522715 NM_133264 NP_573571
Function Plays an active role in the formation of cell surface protrusions downstream of activated PDGFB receptors. Plays an important role in actin-microspike formation through cooperation with WASL. May cooperate with WASP and WASL to induce mobilization and reorganization of the actin filament system. {ECO:0000269|PubMed:11829459, ECO:0000269|PubMed:12213210}.
Subcellular Location Cytoplasm, cytoskeleton {ECO:0000269|PubMed:11829459, ECO:0000269|PubMed:12213210}. Note=Localized to stress fibers and bundles of actin filaments.
Tissue Specificity Expressed mainly in brain, colon, lung and stomach (at protein level). Ubiquitously expressed, with high expression in brain, kidney, lung, and placenta. {ECO:0000269|PubMed:11829459, ECO:0000269|PubMed:12213210}.

WIRE (P-13) - sc-160920 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
WIRE (P-13): sc-160920. Western blot analysis of WIRE expression in HeLa (A), Ramos (B) and U-87 MG (C) whole cell lysates.

WIRE (Y-13) - sc-160921 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-WIPF2 antibody - ab108132 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab108132 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108132 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-WIPF2 / WIRE Antibody (aa392-419, AP) - LS-C242746 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPF2 / WIRE Antibody (aa392-419, Biotin) - LS-C242747 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPF2 / WIRE Antibody (aa392-419, APC) - LS-C242748 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPF2 / WIRE Antibody (aa392-419, FITC) - LS-C242750 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPF2 / WIRE Antibody (aa392-419, PE) - LS-C242751 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPF2 / WIRE Antibody (aa392-419, Azide-free, HRP) - LS-C247253 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPF2 / WIRE Antibody (aa392-419) - LS-C326362 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPF2 / WIRE Antibody (aa323-372) - LS-C116493 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
WIPF2 antibody LS-C116493 Western blot of HepG2 cell lysate.

Anti-WIPF2 / WIRE Antibody (aa392-419) - LS-C160624 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
WIPF2 antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-WIPF2 / WIRE Antibody (aa191-240) - LS-C201603 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human Jurkat. WIPF2 antibody dilution 1.0 ug/ml.

WIRE Antibody - NBP1-86856 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: WIRE Antibody [NBP1-86856] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

WIRE Antibody - NBP1-86858 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

WIRE Antibody - NBP1-86857 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: WIRE Antibody [NBP1-86857] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4

WIPF2 Polyclonal Antibody - bs-9869R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-WIPF2 Polyclonal Antibody, Unconjugated (bs-9869R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

Anti-WIPF2 antibody produced in rabbit - HPA024467 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-WIPF2: <B>Cat. No. HPA024467</B>: Immunohistochemical staining of human rectum shows distinct cytoplasmic positivity in glandular cells.

Anti-WIPF2 antibody produced in rabbit - HPA024000 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-WIPF2: <B>Cat. No. HPA024000</B>: Immunohistochemical staining of human duodenum shows nuclear and cytoplasmic positivity in glandular cells.

Anti-WIPF2 antibody produced in rabbit - HPA024001 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-WIPF2: <B>Cat. No. HPA024001</B>: Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.

WIPF2 antibody - middle region (ARP52556_P050) - ARP52556_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

WIPF2 Antibody - C-terminal region (ARP52557_P050) - ARP52557_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

WIPF2 Antibody - C-terminal region (OAAB00997) - OAAB00997 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

WIPF2-Antibody-C-term - AP10977b from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
WIPF2 Antibody (C-term) (Cat. #AP10977b) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the WIPF2 antibody detected the WIPF2 protein (arrow).

WIPF2 antibody - 70R-21321 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
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