MED24

Gene Symbol MED24
Entrez Gene 9862
Alt Symbol ARC100, CRSP100, CRSP4, DRIP100, THRAP4, TRAP100
Species Human
Gene Type protein-coding
Description mediator complex subunit 24
Other Description CRSP complex subunit 4|activator-recruited cofactor 100 kDa component|cofactor required for Sp1 transcriptional activation subunit 4|cofactor required for Sp1 transcriptional activation, subunit 4, 100kDa|mediator of RNA polymerase II transcription subunit 24|mediator of RNA polymerase II transcription, subunit 24 homolog|thyroid hormone receptor associated protein 4|thyroid hormone receptor-associated protein 4|thyroid hormone receptor-associated protein complex 100 kDa component|vitamin D3 receptor-interacting protein complex 100 kDa component|vitamin D3 receptor-interacting protein complex component DRIP100
Swissprots A8K4S5 Q9NNY5 Q14143 B3KMR9 O75448
Accessions CAH05649 EAW60629 EAW60630 EAW60631 O75448 AF055995 AAC39855 AF277379 AAF78764 AK022508 BAG51081 AK291040 BAF83729 AK293309 BAG56829 AK299864 BAG61718 AK300992 BAG62611 AK303502 BAG64535 AK303745 BAG64717 BC011375 AAH11375 D50920 BAA09479 DB144359 DQ891082 ABM82008 DQ894264 ABM85190 EU392533 ACB88915 EU392534 ACB88916 EU392535 ACB88917 EU392536 ACB88918 EU392537 ACB88919 EU392538 ACB88920 EU392539 ACB88921 EU392540 ACB88922 EU392541 ACB88923 EU392542 ACB88924 EU392543 ACB88925 EU392544 ACB88926 EU392545 ACB88927 EU392546 ACB88928 EU392547 ACB88929 EU392548 EU392549 EU392550 EU392551 EU392552 EU392553 EU392554 XM_005257874 XP_005257931 XM_006722204 XP_006722267 XM_006722206 XP_006722269 XM_006722207 XP_006722270 XM_011525529 XP_011523831 XM_011525530 XP_011523832 XM_011525531 XP_011523833 XM_011525532 XP_011523834 XM_011525533 XP_011523835 XM_011525534 XP_011523836 XM_011525535 XP_011523837 XM_011525536 XP_011523838 XM_011525537 XP_011523839 XM_011525538 XP_011523840 XR_934627 NM_001079518 NP_001072986 NM_001267797 NP_001254726 NM_014815 NP_055630 NR_052017
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. {ECO:0000269|PubMed:12218053, ECO:0000269|PubMed:16595664}.
Subcellular Location Nucleus {ECO:0000305}.
Tissue Specificity Ubiquitous. Abundant in skeletal muscle, heart and placenta. {ECO:0000269|PubMed:9653119}.

TRAP100 (C-16) - sc-5338 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-TRAP100 antibody - ab194951 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-TRAP100 antibody (ab194951) at 1/500 dilution + 293 cell extract

Anti-TRAP100 antibody - ab65110 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
These images show Huvec cell line stained with ab65110 at a 1/500 dilution, with and without the immunising peptide.

Anti-MED24 / TRAP100 Antibody (aa801-850) - LS-C118711 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of HUVEC cells, using MED24 Antibody. The picture on the right is treated with the synthesized peptide.

Anti-MED24 / TRAP100 Antibody (Internal) - LS-C55586 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.05 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-MED24 / TRAP100 Antibody (aa939-989) - LS-C288267 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse TRAP100/MED24 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate (50 ug for WB) from MCF7 (7), PANC1 (P), 293T (T) and mouse NIH3T3 (M) cells. For IP, whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells was used. Antibody: Affinity purified rabbit anti-TRAP100/MED24 antibody used for WB at 1 ug/ml (A and B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Anti-MED24 / TRAP100 Antibody (aa801-850) IHC-plus™ - LS-B11072 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MED24 / TRAP100 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11072 concentration 5 ug/ml.

MED24 Antibody - NB100-92293 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: MED24 Antibody [NB100-92293] - Analysis of MED24 at 1:500 dilution. Lane 1 - HEK293T whole cell lysate, Lane 2 - Raw264.7 whole cell lysate, Lane 3 - H9C2 whole cell lysate.

MED24 Antibody - NB100-74599 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: MED24 Antibody [NB100-74599] - Detection of Human and Mouse TRAP100/MED24 on HeLa whole cell lysate using NB100-74599.

MED24 Antibody - NBP1-06089 from Novus Biologicals

Host Goat
Clonality Polyclonal
Western Blot: MED24 Antibody [NBP1-06089] - (0.05ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-MED24 (821-835) antibody produced in rabbit - SAB1100742 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-MED24 (821-835) : <B>Cat. No. SAB1100742</B>: Immunofluorescence of HUVEC cells using MED24 (821-835) , Cat. No. SAB1100742 (red) at a 1:50 dilution, taken at 40x magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-MED24 antibody produced in rabbit - HPA039547 from Sigma-Aldrich

Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MED24: <B>Cat. No. HPA039547</B>: Immunohistochemical staining of human fallopian tube shows strong positivity in cilia.

Anti-MED24, C-Terminal antibody produced in rabbit - SAB4503717 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-MED24, C-Terminal: <B>Cat. No. SAB4503717</B>: Immunofluorescence of HuvEc cells using Anti-MED24, C-Terminal antibody. Cells on the left were treated with the Anti-MED24, C-Terminal antibody. Cells on the right (negative control) were treated with both Anti-MED24, C-Terminal antibody and the synthesized immunogen peptide.

Anti-THRAP4/MED24 antibody produced in goat - SAB2501037 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-THRAP4 / MED24: <B>Cat. No. SAB2501037</B>: (0.05 μg/mL) staining of Human Heart lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MED24 Antibody (OAAF02055) - OAAF02055 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

MED24 antibody - AP06644PU-N from Acris Antibodies

Prices $330.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot (WB) analysis of TRAP100 antibody (Cat.-No.: AP06644PU-N) in extracts from 293 cells.

Anti-TRAP100 Antibody - AC21-1292 from Abcore

Prices $359.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-TRAP100 Antibody (AMCA) - AC21-1292-01 from Abcore

Prices $495.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-TRAP100 Antibody (AP) - AC21-1292-02 from Abcore

Prices $505.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-TRAP100 Antibody (APC) - AC21-1292-03 from Abcore

Prices $515.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-TRAP100 Antibody (APC-Cy5.5) - AC21-1292-04 from Abcore

Prices $539.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-TRAP100 Antibody (APC-Cy7) - AC21-1292-05 from Abcore

Prices $539.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-TRAP100 Antibody (Avidin) - AC21-1292-06 from Abcore

Prices $519.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-TRAP100 Antibody (Biotin) - AC21-1292-07 from Abcore

Prices $519.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-TRAP100 Antibody (BPE) - AC21-1292-08 from Abcore

Prices $499.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
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