| Gene Symbol | POLR3A |
|---|---|
| Entrez Gene | 11128 |
| Alt Symbol | ADDH, HLD7, RPC1, RPC155, hRPC155 |
| Species | Human |
| Gene Type | protein-coding |
| Description | polymerase (RNA) III (DNA directed) polypeptide A, 155kDa |
| Other Description | DNA-directed RNA polymerase III largest subunit|DNA-directed RNA polymerase III subunit A|DNA-directed RNA polymerase III subunit RPC1|RNA polymerase III 155 kDa subunit|RNA polymerase III subunit C1|RNA polymerase III subunit C160|RNA polymerase III subunit RPC155-D |
| Swissprots | Q8TCW5 Q8IW34 O14802 |
| Accessions | EAW54617 O14802 AF021351 AAB86536 AK024744 AK026023 AK300329 BAH13260 AY091459 AAM12029 AY091460 AAM12030 AY091461 AAM12031 AY091462 AAM12032 BC014399 AAH14399 BC041089 AAH41089 CA449326 DA103416 DA353439 NM_007055 NP_008986 |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsR |
| Subcellular Location | Nucleus. |
| Tissue Specificity | Expressed in the brain, in the cortex and the white matter (at protein level). {ECO:0000269|PubMed:21855841}. |
| Top Pathways | Cytosolic DNA-sensing pathway, Epstein-Barr virus infection, Purine metabolism, RNA polymerase, Pyrimidine metabolism |
POLR3A CRISPR/Cas9 KO Plasmid (h) - sc-406851 from Santa Cruz Biotechnology
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POLR3A HDR Plasmid (h) - sc-406851-HDR from Santa Cruz Biotechnology
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POLR3A Double Nickase Plasmid (h) - sc-406851-NIC from Santa Cruz Biotechnology
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POLR3A Double Nickase Plasmid (h2) - sc-406851-NIC-2 from Santa Cruz Biotechnology
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POLR3A CRISPR Activation Plasmid (h) - sc-406851-ACT from Santa Cruz Biotechnology
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POLR3A CRISPR Activation Plasmid (h2) - sc-406851-ACT-2 from Santa Cruz Biotechnology
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POLR3A Lentiviral Activation Particles (h) - sc-406851-LAC from Santa Cruz Biotechnology
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POLR3A Lentiviral Activation Particles (h2) - sc-406851-LAC-2 from Santa Cruz Biotechnology
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POLR3A siRNA (h) - sc-90684 from Santa Cruz Biotechnology
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POLR3A shRNA Plasmid (h) - sc-90684-SH from Santa Cruz Biotechnology
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POLR3A shRNA (h) Lentiviral Particles - sc-90684-V from Santa Cruz Biotechnology
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POLR3A RNAi - H00011128-R01 from Novus Biologicals
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MISSION® esiRNA - EHU037881 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17126 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17126 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR09455 from Sigma-Aldrich
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POLR3A siRNA (Human) - CRH7612 from Cohesion Biosciences
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shRNA set against Human POLR3A (NM_007055.3) - SHH379528 from Creative biogene
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Human POLR3A siRNA - orb268114 from Biorbyt
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POLR3A shRNA Plasmid (Human) - CHH7612 from Cohesion Biosciences
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POLR3A miRNA 3'UTR clone - MiUTR3H-13189 from Creative biogene
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