DPH7

Gene Symbol DPH7
Entrez Gene 92715
Alt Symbol C9orf112, RRT2, WDR85
Species Human
Gene Type protein-coding
Description diphthamide biosynthesis 7
Other Description WD repeat domain 85|WD repeat-containing protein 85|diphthamide biosynthesis protein 7|diphthine methyltransferase
Swissprots Q96AB7 Q9BTV6
Accessions CAF86364 CCQ43831 EAW88400 EAW88401 Q9BTV6 AJ420445 AK075115 BAC11411 AK094389 AK307180 AK308998 AK310226 AK310987 BC003123 AAH03123 BC016349 BC017335 AAH17335 BC040173 AAH40173 HQ448236 ADQ32716 XM_005266123 XP_005266180 XM_005266124 XP_005266181 XM_005266125 XP_005266182 XM_005266126 XP_005266183 XM_005266128 XP_005266185 XM_005266129 XP_005266186 XM_005266130 XP_005266187 XM_011519188 XP_011517490 XM_011519189 XP_011517491 XM_011519190 XP_011517492 XM_011519191 XP_011517493 XM_011519192 XP_011517494 XM_011519193 XP_011517495 XM_011519194 XP_011517496 XM_011519195 XP_011517497 XM_011519196 XP_011517498 XM_011519197 XP_011517499 XM_011519198 XP_011517500 XM_011519199 XP_011517501 XM_011519200 XP_011517502 NM_138778 NP_620133
Function Catalyzes the demethylation of diphthine methyl ester to form diphthine, an intermediate diphthamide biosynthesis, a post- translational modification of histidine which occurs in translation elongation factor 2 (EEF2) which can be ADP- ribosylated by diphtheria toxin and by Pseudomonas exotoxin A (Eta). {ECO:0000250|UniProtKB:P38332, ECO:0000269|PubMed:19965467, ECO:0000269|PubMed:23486472}.

WDR85 (N-18) - sc-249340 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-WDR85 antibody - ab111910 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-WDR85 antibody (ab111910) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

Anti-WDR85 antibody - ab150815 from Abcam

Prices $443.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-WDR85 antibody (ab150815) at 1/250 dilutionLane 1 : RT-4 cell lysateLane 2 : U-251 MG cell lysateLane 3 : Human plasmaLane 4 : Human liver lysateLane 5 : Human tonsil lysatedeveloped using the ECL technique

Anti-WDR85 antibody - ab168318 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-WDR85 antibody (ab168318) at 1/500 dilutionLane 1 : WDR85 transfected 293T cellsLane 2 : Non transfected 293T cellsLysates/proteins at 15 µl per lane.SecondaryGoat anti-Mouse IgG

Anti-WDR85 antibody - N-terminal - ab177340 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-WDR85 antibody - N-terminal (ab177340) at 1 µg/ml + Jurkat whole cell lysate at 10 µg

Anti-DPH7 / WDR85 Antibody (aa32-51, AP) - LS-C273541 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DPH7 / WDR85 Antibody (aa32-51, APC) - LS-C273542 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DPH7 / WDR85 Antibody (aa32-51, Biotin) - LS-C273543 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DPH7 / WDR85 Antibody (aa32-51, PE) - LS-C273546 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DPH7 / WDR85 Antibody (aa32-51) - LS-C330296 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DPH7 / WDR85 Antibody (aa32-51, FITC) - LS-C273544 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DPH7 / WDR85 Antibody (aa32-51, Azide-free, HRP) - LS-C273545 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DPH7 / WDR85 Antibody (aa23-51) - LS-C160607 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
WDR85 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The WDR85 antibody detected the WDR85 protein (arrow).

Anti-DPH7 / WDR85 Antibody (aa22-71) - LS-C170660 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

WDR85 Antibody - NBP1-86714 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

WDR85 Antibody - NBP2-20902 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: WDR85 Antibody [NBP2-20902] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.

WDR85 Antibody - H00092715-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: WDR85 Antibody [H00092715-B01] - analysis of C9orf112 expression in transfected 293T cell line by C9orf112 MaxPab polyclonal antibody. Lane 1: C9orf112 transfected lysate (49.72 KDa). Lane 2: Non-transfected lysate.

Anti-DPH7 antibody produced in rabbit - HPA022911 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-WDR85: <B>Cat. No. HPA022911</B>: Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.

Anti-WDR85 (51-65) antibody produced in rabbit - SAB1102941 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human WDR85 was separated on SDS-PAGE, blotted with Anti-WDR85 (51-65) (<B>Cat. No. SAB1102941</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000

Anti-WDR85 (386-400) antibody produced in rabbit - SAB1102942 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human WDR85 was separated on SDS-PAGE, blotted with Anti-WDR85 (386-400) (<B>Cat. No. SAB1102942</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000

WDR85 Antibody - N-terminal region (ARP69141_P050) - ARP69141_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

WDR85-Antibody-N-term - AP12477a from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
WDR85 Antibody (N-term) (Cat. #AP12477a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the WDR85 antibody detected the WDR85 protein (arrow).

WDR85 Antibody - N-terminal region (OAAB08747) - OAAB08747 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

WDR85 Antibody - abx026840 from Abbexa

Host Rabbit
Clonality Polyclonal

WDR85 (N-term) antibody - AP54555PU-N from Acris Antibodies

Prices $330.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
WDR85 Antibody (N-term) (AP54555PU-N) western blot analysis in HepG2 cell line lysates (35 µg/lane). This demonstrates the WDR85 antibody detected the WDR85 protein (arrow).
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