DGCR8

Gene Symbol DGCR8
Entrez Gene 54487
Alt Symbol C22orf12, DGCRK6, Gy1, pasha
Species Human
Gene Type protein-coding
Description DGCR8 microprocessor complex subunit
Other Description DiGeorge syndrome critical region 8|DiGeorge syndrome critical region gene 8|microprocessor complex subunit DGCR8
Swissprots Q6MZE9 Q9NRW2 Q6DCB2 Q6Y2L0 Q9H6T7 Q9H6L8 B2R8G1 Q8WYQ5 Q96GP8 Q96G39
Accessions BAB83032 CBM42911 CBV35644 EAX02998 EAX02999 EAX03000 Q8WYQ5 AA737782 AF165527 AAF82263 AK025427 AK025539 BAB15165 AK025780 BAB15238 AK094381 AK222796 BAD96516 AK313357 BAG36158 AM392653 CAL37531 AY189282 AAO86726 BC009323 AAH09323 BC009984 AAH09984 BC037564 BC071568 BC078147 AAH78147 BQ001948 BQ227899 BX649187 CAE46205 CR456356 CAG30242 CU013066 CAK54497 CU013354 CAK54796 DA433651 XM_006724268 XP_006724331 NM_001190326 NP_001177255 NM_022720 NP_073557
Function Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri- miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri- miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self- ren
Subcellular Location Nucleus. Nucleus, nucleolus. Note=Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies.
Tissue Specificity Ubiquitously expressed. {ECO:0000269|PubMed:12705904}.

DGCR8 (D78E4) Rabbit mAb - 6914 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D78E4
Western blot analysis of extracts from 293 and HeLa cells using DGCR8 (D78E4) Rabbit mAb.

DGS8 (E-10) - sc-377249 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DGS8 (E-10): sc-377249. Western blot analysis of DGS8 expression in Jurkat (A), CTLL-2 (B), HuT 78 (C) and H9 (D) whole cell lysates.

DGS8 (H-6) - sc-271259 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DGS8 (H-6): sc-271259. Western blot analysis of DGS8 expression in HeLa (A) and Jurkat (B) whole cell lysates.

DGS8 (N-19) - sc-48473 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DGS8 (N-19): sc-48473. Western blot analysis of DGS8 expression in non-transfected: sc-117752 (A) and human DGS8 transfected: sc-117436 (B) 293T whole cell lysates.

Anti-DGCR8 antibody - ab90579 from Abcam

Prices $390.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DGCR8 with ab90579 at 1/200 (1µg/ml). Detection: DAB.

Anti-DGCR8 antibody - ab36865 from Abcam

Prices $382.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DGCR8 antibody (ab36865) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-DGCR8 antibody - ab135733 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-DGCR8 antibody (ab135733) at 1/100 dilution + NCI-H460 lysate at 35 µg

Anti-DGCR8 antibody - ab90649 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling DGCR8 with ab90649 at 1/1000 (1µg/ml). Detection: DAB.

Anti-DGCR8 antibody - ab85136 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab85136 at a 1/100 dilution staining DGCR8 in Human spleen tissue (paraffin section).

Anti-DGCR8 Antibody (aa446-460) - LS-C112717 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-DGCR8 Antibody (Internal, AP) - LS-C276846 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DGCR8 Antibody (Internal, APC) - LS-C276847 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DGCR8 Antibody (Internal, Biotin) - LS-C276848 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DGCR8 Antibody (Internal, FITC) - LS-C276849 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DGCR8 Antibody (Internal, Azide-free, HRP) - LS-C276850 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DGCR8 Antibody (Internal, PE) - LS-C276851 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DGCR8 Antibody (Internal) - LS-C54718 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
DGCR8 antibody (0.1 ug/ml) staining of NIH3T3 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-DGCR8 Antibody (clone 4G3) - LS-C134081 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4G3
Detection limit for recombinant GST tagged DGCR8 is 0.1 ng/ml as a capture antibody.

Anti-DGCR8 Antibody - LS-C311087 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DGCR8 Antibody - LS-C307228 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-DGCR8 Antibody - LS-C137005 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DGCR8 Antibody (aa1-50) - LS-C286892 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human DGCR8 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DGCR8 used at a dilution of 1:250.

Anti-DGCR8 Antibody (Internal) - LS-C327211 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DGCR8 Antibody (aa400-450) - LS-C289205 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human DGCR8 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DGCR8 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

Anti-DGCR8 Antibody (Internal) - LS-C345786 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
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