POLR2B

Gene Symbol POLR2B
Entrez Gene 5431
Alt Symbol POL2RB, RPB2, hRPB140
Species Human
Gene Type protein-coding
Description polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
Other Description DNA-directed RNA polymerase II 140 kDa polypeptide|DNA-directed RNA polymerase II subunit B|DNA-directed RNA polymerase II subunit RPB2|RNA polymerase II second largest subunit|RNA polymerase II subunit 2|RNA polymerase II subunit B2
Swissprots P30876 Q8IZ61 A8K1A8
Accessions AAB23139 ABX80206 EAX05519 P30876 AF055028 AAC09367 AK098271 AK289823 BAF82512 AK294901 BAG57990 AK295132 BAG58155 AK299090 BAG61152 BC023503 AAH23503 BC046499 BU619780 DC323029 X63563 CAA45124 NM_000938 NP_000929 NM_001303268 NP_001290197 NM_001303269 NP_001290198
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity). {ECO:0000250}.
Subcellular Location Nucleus {ECO:0000269|PubMed:9852112}.
Top Pathways Huntington's disease, Epstein-Barr virus infection, Purine metabolism, RNA polymerase, Pyrimidine metabolism

POLR2B (E-12) - sc-166803 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
POLR2B (E-12): sc-166803. Western blot analysis of POLR2B expression in HeLa nuclear extract.

POLR2B (H-201) - sc-67318 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
POLR2B (H-201): sc-67318. Western blot analysis of POLR2B expression in 293T whole cell lysate (A) and Ramos nuclear extract (B).

POLR2B (N-15) - sc-54679 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
POLR2B (N-15): sc-54679. Western blot analysis of POLR2B expression in HeLa (A) and Ramos (B) nuclear extracts.

POLR2B (S-20) - sc-55039 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
POLR2B (S-20): sc-55039. Western blot analysis of POLR2B expression in HeLa (A), 3611-RF (B), IMR-32 (C), Jurkat (D) and Sol8 (E) nuclear extracts.

POLR2B (T-15) - sc-55040 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-RPB2 antibody - ab96797 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-RPB2 antibody (ab96797) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

Anti-RPB2 antibody - ab10332 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-RPB2 antibody - ab123568 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-RPB2 antibody (ab123568) at 1/100 dilution + 293 cell lysate at 35 µg

Anti-RPB2 antibody - ChIP Grade - ab10338 from Abcam

Prices $392.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-POLR2B / RPB2 Antibody (C-Terminus) - LS-C93354 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-POLR2B / RPB2 Antibody (C-Terminus) - LS-C93355 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR2B / RPB2 Antibody (aa796-826, AP) - LS-C239288 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR2B / RPB2 Antibody (aa796-826, APC) - LS-C239289 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR2B / RPB2 Antibody (aa796-826, Biotin) - LS-C239290 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR2B / RPB2 Antibody (aa119-168) - LS-C31479 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
HepG2 Cell Lysate

Anti-POLR2B / RPB2 Antibody (aa796-826, FITC) - LS-C239291 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR2B / RPB2 Antibody (aa796-826, PE) - LS-C239295 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR2B / RPB2 Antibody (aa796-826, Azide-free, HRP) - LS-C248521 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR2B / RPB2 Antibody (aa75-125) - LS-C287242 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR2B / RPB2 Antibody (aa796-826) - LS-C324169 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR2B / RPB2 Antibody (aa797-826) - LS-C163849 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
POLR2B Antibody western blot of 293 cell line lysates (35 ug/lane). The POLR2B antibody detected the POLR2B protein (arrow).

Anti-POLR2B / RPB2 Antibody - LS-C334391 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of A431 cell line, using POLR2B antibody.

Anti-POLR2B / RPB2 Antibody (aa450-500) - LS-C287244 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Rpb2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (50 ug for WB; 10 cm plate for IP) from 293T cells. Antibodies: Affinity purified rabbit anti-Rpb2 used at the indicated concentration (A), 1 ug/ml (B) or 10 ug/10 cm plate for IP. Detection: Chemiluminescence with exposure times as indicated (A) or 10 seconds (B).

Anti-POLR2B / RPB2 Antibody (aa1-172) - LS-C185491 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded NCIN87 xenograft using POLR2B antibody at 1:500 dilution.

Anti-POLR2B / RPB2 Antibody (aa805-854) IHC-plus™ - LS-B1557 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-POLR2B / RPB2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1557 concentration 5 ug/ml.
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