| Gene Symbol | POLR2B |
|---|---|
| Entrez Gene | 5431 |
| Alt Symbol | POL2RB, RPB2, hRPB140 |
| Species | Human |
| Gene Type | protein-coding |
| Description | polymerase (RNA) II (DNA directed) polypeptide B, 140kDa |
| Other Description | DNA-directed RNA polymerase II 140 kDa polypeptide|DNA-directed RNA polymerase II subunit B|DNA-directed RNA polymerase II subunit RPB2|RNA polymerase II second largest subunit|RNA polymerase II subunit 2|RNA polymerase II subunit B2 |
| Swissprots | P30876 Q8IZ61 A8K1A8 |
| Accessions | AAB23139 ABX80206 EAX05519 P30876 AF055028 AAC09367 AK098271 AK289823 BAF82512 AK294901 BAG57990 AK295132 BAG58155 AK299090 BAG61152 BC023503 AAH23503 BC046499 BU619780 DC323029 X63563 CAA45124 NM_000938 NP_000929 NM_001303268 NP_001290197 NM_001303269 NP_001290198 |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity). {ECO:0000250}. |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:9852112}. |
| Top Pathways | Huntington's disease, Epstein-Barr virus infection, Purine metabolism, RNA polymerase, Pyrimidine metabolism |
POLR2B CRISPR/Cas9 KO Plasmid (h) - sc-402591 from Santa Cruz Biotechnology
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POLR2B HDR Plasmid (h) - sc-402591-HDR from Santa Cruz Biotechnology
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RPB2 Double Nickase Plasmid (h) - sc-402591-NIC from Santa Cruz Biotechnology
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POLR2B Double Nickase Plasmid (h2) - sc-402591-NIC-2 from Santa Cruz Biotechnology
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RPB2 CRISPR Activation Plasmid (h) - sc-402591-ACT from Santa Cruz Biotechnology
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POLR2B CRISPR Activation Plasmid (h2) - sc-402591-ACT-2 from Santa Cruz Biotechnology
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RPB2 Lentiviral Activation Particles (h) - sc-402591-LAC from Santa Cruz Biotechnology
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POLR2B Lentiviral Activation Particles (h2) - sc-402591-LAC-2 from Santa Cruz Biotechnology
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POLR2B siRNA (h) - sc-62962 from Santa Cruz Biotechnology
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POLR2B shRNA Plasmid (h) - sc-62962-SH from Santa Cruz Biotechnology
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POLR2B shRNA (h) Lentiviral Particles - sc-62962-V from Santa Cruz Biotechnology
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RPB2 RNAi - H00005431-R01 from Novus Biologicals
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MISSION® esiRNA - EHU048971 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17112 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17112 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR00967 from Sigma-Aldrich
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POLR2B siRNA (Human) - CRH3634 from Cohesion Biosciences
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shRNA set against Human POLR2B (NM_000938.1) - SHH379388 from Creative biogene
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shRNA set against Human POLR2B(NM_000938.1) - SHR127216 from Creative biogene
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POLR2B shRNA Plasmid (Human) - CHH3634 from Cohesion Biosciences
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POLR2B miRNA 3'UTR clone - MiUTR1H-08072 from Creative biogene
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