GPX4

Gene Symbol GPX4
Entrez Gene 2879
Alt Symbol GPx-4, GSHPx-4, MCSP, PHGPx, snGPx, snPHGPx
Species Human
Gene Type protein-coding
Description glutathione peroxidase 4
Other Description phospholipid hydroperoxidase|phospholipid hydroperoxide glutathione peroxidase, mitochondrial|sperm nucleus glutathione peroxidase
Swissprots P36969 O43381 Q9UPK2 Q6PJ59
Accessions AAC03239 AAC28920 AAC32261 AAP72965 EAW69543 EAW69544 EAW69545 EAW69546 P36969 AI274351 AK225859 AL558552 BC010157 AAH10157 BC011836 AAH11836 BC021567 AAH21567 BC022071 AAH22071 BC032695 AAH32695 BC039849 AAH39849 BC046163 AAH46163 BC058329 BI561548 BM764133 BQ648717 CF127510 HY049693 X71973 CAA50793 NM_001039847 NP_001034936 NM_001039848 NP_001034937 NM_002085 NP_002076
Function Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage (By similarity). {ECO:0000250}.
Subcellular Location Isoform Mitochondrial: Mitochondrion.
Tissue Specificity Present primarily in testis.
Top Pathways Glutathione metabolism

GPx-4 (B-12) - sc-166120 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GPx-4 (B-12): sc-166120. Western blot analysis of GPx-4 expression in rat testis tissue extract.

GPx-4 (C-14) - sc-27531 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GPx-4 (C-14): sc-27531. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic and nuclear staining of cells in seminiferous ducts and Leydig cells.

GPx-4 (E-12) - sc-166570 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GPx-4 (E-12): sc-166570. Western blot analysis of GPx-4 expression in non-transfected: sc-117752 (A) and human GPx-4 transfected: sc-115819 (B) 293T whole cell lysates.

GPx-4 (D-3) - sc-166437 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GPx-4 (D-3): sc-166437. Western blot analysis of GPx-4 expression in HeLa whole cell lysate (A) and mouse testis tissue extract (B).

GPx-4 (H-90) - sc-50497 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GPx-4 (H-90): sc-50497. Western blot analysis of GPx-4 expression in mouse testis (A) and rat testis (B) tissue extracts.

GPx-4 (P-16) - sc-55199 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

GPx-4 (N-18) - sc-27529 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GPx-4 (N-18): sc-27529. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

GPx-4 (Y-13) - sc-55200 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Glutathione Peroxidase 4 antibody - ab185689 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Glutathione Peroxidase 4 antibody (ab185689) at 1/500 dilution + Mouse kidney cell line extract

Anti-Glutathione Peroxidase 4 antibody - ab40993 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Glutathione Peroxidase 4 antibody (ab40993) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 4 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 5 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 6 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 7 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Glutathione Peroxidase 4 antibody - ab42808 from Abcam

Prices $372.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-Glutathione Peroxidase 4 antibody - ab41787 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Glutathione Peroxidase 4 antibody (ab41787) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 4 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 5 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 6 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Glutathione Peroxidase 4 antibody - ab116703 from Abcam

Prices $378.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-Glutathione Peroxidase 4 antibody (ab116703) at 0.1 µg/ml + Human Testis lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-Glutathione Peroxidase 4 antibody [1H11] - ab16739 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1H11
Ab16739 at a concentration of 1µg/ml lysate immunoprecipitating approx. 22kDa Glutathione peroxidase 4 from HL60 cell lysate (lane 2). Ab16800 was used to stain the Westrn blot. Lane 1) contains non-immunoprecipitated HL60 cell lysate.

Anti-Glutathione Peroxidase 4 antibody [7A4] - ab16737 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7A4
ICC/IF image of ab16737 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16737, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] - ab125066 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPNCIR144
ab125066 staining Glutathione Peroxidase 4 in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081 an Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Nuclear staining was carried out with DAPI.See Abreview

Anti-GPX4 / MCSP Antibody (aa184-196) IHC-plus™ - LS-B3246 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-GPX4 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3246 concentration 5 ug/ml.

Anti-GPX4 / MCSP Antibody (aa81-93) IHC-plus™ - LS-B1596 from LifeSpan Bioscience

Prices $395.00
Sizes 1 ea
Host Rabbit
Clonality Polyclonal
Anti-GPX4 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1596 concentration 10 ug/ml.

Anti-GPX4 / MCSP Antibody (clone 1H11) IHC-plus™ - LS-B7835 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 1H11
Anti-GPX4 / MCSP antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7835 dilution 1:200.

Anti-GPX4 / MCSP Antibody (C-Terminus) - LS-C56834 from LifeSpan Bioscience

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-GPX4 / MCSP Antibody - LS-C61931 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-GPX4 / MCSP Antibody (N-Terminus) - LS-C123624 from LifeSpan Bioscience

Prices $575.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPX4 / MCSP Antibody (C-Terminus) - LS-C18861 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-GPx4 Antibody - Western Blot. Western blot of affinity purified anti-GPx4 to detect GPx4 in testis extract (arrow). tissue extract (40 ug) was electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1000 dilution. Personal Communication, Dolph Hatfield, CCR-NCI, Bethesda, MD.

Anti-GPX4 / MCSP Antibody - LS-C129320 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-GPX4 / MCSP Antibody - LS-C125672 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
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