DSN1

Gene Symbol	DSN1
Entrez Gene	79980
Alt Symbol	C20orf172, KNL3, MIS13, dJ469A13.2, hKNL-3
Species	Human
Gene Type	protein-coding
Description	DSN1, MIS12 kinetochore complex component
Other Description	DSN1, MIND kinetochore complex component, homolog\|kinetochore null 3 homolog\|kinetochore-associated protein DSN1 homolog
Swissprots	Q9H8P4 Q5JW55 Q5JW56 B4DWT2 E1P5U9 Q9H410
Accessions	EAW76101 EAW76102 EAW76103 EAW76104 EAW76105 EAW76106 EAW76107 EAW76108 Q9H410 AA534892 AK023408 BAB14564 AK093031 BAC04024 AK290738 BAF83427 AK301671 BAG63144 AK301840 BAG63283 BC026011 AAH26011 BC035821 BC058899 AAH58899 HQ448110 ADQ32591 XM_006723876 XP_006723939 NM_001145315 NP_001138787 NM_001145316 NP_001138788 NM_001145317 NP_001138789 NM_001145318 NP_001138790 NM_024918 NP_079194
Function	Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. {ECO:0000269\|PubMed:15502821, ECO:0000269\|PubMed:16585270}.
Subcellular Location	Nucleus {ECO:0000269\|PubMed:15502821, ECO:0000269\|PubMed:16585270}. Chromosome, centromere, kinetochore {ECO:0000269\|PubMed:15502821, ECO:0000269\|PubMed:16585270}. Note=Associated with the kinetochore. {ECO:0000269\|PubMed:15502821, ECO:0000269\|PubMed:16585270}.

DSN1 (Q-16) - sc-85549 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

DSN1 (Q-16): sc-85549. Western blot analysis of DSN1 expression in HeLa (A), Jurkat (B), NIH/3T3 (C), CCRF-CEM (D) and IMR-32 (E) nuclear extracts and mouse brain tissue extract (F).

DSN1 (S-17) - sc-85550 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

DSN1 (S-17): sc-85550. Western blot analysis of DSN1 expression in Jurkat (A), CCRF-CEM (B) and IMR-32 (C) nuclear extracts.

Anti-DSN1 antibody - ab201158 from Abcam

Prices	$359.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-DSN1 antibody (ab201158) at 1/500 dilutionLane 1 : HepG2 cell lysateLane 2 : MCF7 cell lysateLane 3 : 7721 cell lysateLane 4 : 7703 cell lysateLane 5 : 231 cell lysateLane 6 : 435s cell lysate

Anti-DSN1 antibody - ab80062 from Abcam

Prices	$370.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DSN1 antibody - ab134223 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DSN1 antibody (ab134223) at 1 µg/ml + HepG2 cell lysate at 10 µg

Anti-DSN1 antibody - ab134244 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DSN1 antibody (ab134244) at 1 µg/ml + MCF7 cell lysate at 10 µg

Anti-DSN1 antibody - ab77791 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-DSN1 antibody (ab77791) at 1/500 dilution + recombinant fragment (the immunogen) at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/5000 dilutiondeveloped using the ECL technique

Anti-DSN1 Antibody (aa257-286, AP) - LS-C234758 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DSN1 Antibody (aa257-286, APC) - LS-C234760 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DSN1 Antibody (aa257-286, Biotin) - LS-C234759 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DSN1 Antibody (aa59-108) - LS-C152063 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-DSN1 Antibody (aa257-286, FITC) - LS-C234761 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DSN1 Antibody (aa150-199) - LS-C152064 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-DSN1 Antibody (aa257-286, Azide-free, HRP) - LS-C249868 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DSN1 Antibody (aa257-286, PE) - LS-C234766 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DSN1 Antibody (aa257-286) - LS-C167545 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

DSN1 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The DSN1 antibody detected the DSN1 protein (arrow).

Anti-DSN1 Antibody (aa264-295) - LS-C320958 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DSN1 Antibody (aa257-356) - LS-C307897 from LifeSpan Bioscience

Prices	$455.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-DSN1 Antibody (Internal) - LS-C144971 from LifeSpan Bioscience

Prices	$365.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

DSN1 Antibody - NBP1-88302 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: DSN1 Antibody [NBP1-88302] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

DSN1 Antibody (2A7) - H00079980-M01 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2A7

Sandwich ELISA: DSN1 Antibody (2A7) [H00079980-M01] - Detection limit for recombinant GST tagged DSN1 is 1 ng/ml as a capture antibody.

DSN1 Polyclonal Antibody - bs-7796R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DSN1 antibody produced in rabbit - SAB2702119 from Sigma-Aldrich

Prices	$326.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunofluorescence</B><BR/>Anti-DSN1: <B>Cat. No. SAB2702119</B>: Immunofluroescence analysis of paraformaldehyde-fixed HeLa cells using DSN1 antibody at a 1:1,000 dilution with 10% normal donkey serum in PHEM buffer. ACA is used as a marker for inner kinetochore.

Monoclonal Anti-C20ORF172, (C-terminal) antibody produced in mouse - SAB1403374 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2A7

<B>Immunoblotting</B><BR/>Monoclonal Anti-C20orf172: <B>Cat. No. SAB1403374</B>: Antibody concentration: 1 μg/mL. Specific band of ~37 kDa using immunogen protein lysate.

Anti-DSN1 antibody produced in rabbit - HPA002813 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-DSN1: <B>Cat. No. HPA002813</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human vulva/anal skin tissue showing nuclear staining of surface epithelial cells.

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