CHCHD3

Gene Symbol CHCHD3
Entrez Gene 54927
Alt Symbol MINOS3, PPP1R22
Species Human
Gene Type protein-coding
Description coiled-coil-helix-coiled-coil-helix domain containing 3
Other Description MICOS complex subunit MIC19|coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial|mitochondrial inner membrane organizing system 3|protein phosphatase 1, regulatory subunit 22
Swissprots Q9NX63
Accessions EAL24074 EAW83800 EAW83801 EAW83802 EAW83803 Q9NX63 AK000427 BAA91157 AK129533 AK294071 BAH11665 AK310236 BC011596 AAH11596 BC014839 AAH14839 HQ447383 ADQ31869 XM_011516363 XP_011514665 XM_011516364 XP_011514666 XM_011516365 XP_011514667 NM_017812 NP_060282
Function Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. {ECO:0000269|PubMed:22567091}.
Subcellular Location Mitochondrion inner membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}; Intermembrane side {ECO:0000250}. Cytoplasm {ECO:0000269|PubMed:22567091}. Nucleus {ECO:0000269|PubMed:22567091}.
Tissue Specificity Detected at low levels in brain, placenta, lung, liver, kidney and pancreas with increased levels in heart and skeletal muscle. Higher expression in primary lung cancers than in normal lung tissue. {ECO:0000269|PubMed:22567091}.

CHCHD3 (Y-12) - sc-164059 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-CHCHD3 antibody - ab69328 from Abcam

Prices $382.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-CHCHD3 antibody (ab69328) at 1/500 dilution + Human placenta tissue lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-CHCHD3 antibody - ab133427 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Immunohistochemical analysis of formalin fixed, paraffin embedded Human kidney tissue labelling CHCHD3 with ab133427 at 5µg/ml, followed by biotinylated Horse anti-Goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-CHCHD3 Antibody - LS-C306930 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-CHCHD3 Antibody (aa107-156) - LS-C111963 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
CHCHD3 antibody LS-C111963 Western blot of Placenta lysate.

Anti-CHCHD3 Antibody (aa2-227, clone 7G4) - LS-C338750 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7G4
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHCHD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHCHD3.

Anti-CHCHD3 Antibody (aa2-227, clone 2G8) - LS-C336849 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2G8
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHCHD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHCHD3.

Anti-CHCHD3 Antibody (aa35-84) - LS-C111962 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
CHCHD3 antibody LS-C111962 Western blot of HeLa lysate.

Anti-CHCHD3 Antibody (Internal) - LS-C112926 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
CHCHD3 antibody (1 ug/ml) staining of Human Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CHCHD3 Antibody (aa2-227, clone 3E10) - LS-C338777 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E10
IHC of paraffin-embedded Human prostate tissue using anti-CHCHD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min).

Anti-CHCHD3 Antibody (aa2-227, clone 2A6) - LS-C336855 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2A6
IHC of paraffin-embedded Human pancreas tissue using anti-CHCHD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-CHCHD3 Antibody (Internal) IHC-plus™ - LS-B6861 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-CHCHD3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6861 concentration 5 ug/ml.

Anti-CHCHD3 Antibody (aa83-116) - LS-C344345 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of lysate from 293T cell line with CHCHD3 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.

CHCHD3 Antibody - NBP1-83656 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

CHCHD3 Antibody - NBP2-21606 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CHCHD3 Antibody [NBP2-21606] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 12% SDS PAGE gel, diluted at 1:1000.

CHCHD3 Antibody - NBP1-56871 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CHCHD3 Antibody [NBP1-56871] - Titration: 2 ug/ml Positive Control: whole cell protein extracts, immortalized human fibroblasts.

CHCHD3 Antibody - NBP1-56870 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CHCHD3 Antibody [NBP1-56870] - Hela cell lysate, concentration 0.2-1 ug/ml.

CHCHD3 Antibody - H00054927-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: CHCHD3 Antibody [H00054927-B01P] - Analysis of CHCHD3 expression in human placenta.

CHCHD3 Antibody - NBP1-52009 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: CHCHD3 Antibody [NBP1-52009] - (0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CHCHD3 Antibody - NBP1-52008 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: CHCHD3 Antibody [NBP1-52008] - (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CHCHD3 antibody produced in rabbit - HPA042935 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-CHCHD3: <B>Cat. No. HPA042935</B>: Immunohistochemical staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells.

Anti-CHCHD3 antibody produced in goat - SAB2501548 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-CHCHD3 <B>Cat. No. SAB2501548</B>: (0.3 μg/mL) staining of Human Placenta lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CHCHD3 antibody produced in mouse - SAB1400591 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Polyclonal Anti-CHCHD3, antibody produced in mouse, purified immunoglobulin: <B>Cat. No. SAB1400591</B>: Antibody concentration: 1.0 μg/mL using HeLa cells.

CHCHD3 antibody - N-terminal region (ARP57039_P050) - ARP57039_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

CHCHD3 antibody - middle region (ARP57040_P050) - ARP57040_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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