| Gene Symbol | ALOX15 |
|---|---|
| Entrez Gene | 246 |
| Alt Symbol | 12-LOX, 15-LOX-1, 15LOX-1 |
| Species | Human |
| Gene Type | protein-coding |
| Description | arachidonate 15-lipoxygenase |
| Other Description | 12/15-lipoxygenase|15-LOX|15-lipooxygenase-1|arachidonate 12-lipoxygenase, leukocyte-type|arachidonate omega-6 lipoxygenase |
| Swissprots | P16050 Q99657 B7ZA11 A8K2P4 Q8N6R7 |
| Accessions | AAB49305 AAC52118 AAR84235 EAW90427 EAW90428 P16050 AK290309 BAF82998 AK296792 BAH12431 AK296821 BAH12437 AK296904 BAH12452 AK312557 BAG35454 AK316126 BAH14497 BC029032 AAH29032 M23892 AAA36182 M95923 AAA35482 NM_001140 NP_001131 |
| Function | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12- hydroperoxyeicosatetraenoic acid/12-HPETE and 15- hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function throug |
| Subcellular Location | Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Lipid droplet. Note=Predominantly cytosolic; becomes enriched at membranes upon calcium binding. Translocates from the cytosol to the plasma membrane when stimulated by IL13/interleukin-13 and in macrophages binding apoptotic cells. |
| Tissue Specificity | Detected in monocytes and eosinophils (at protein level). Expressed in airway epithelial cells. {ECO:0000269|PubMed:21831839, ECO:0000269|PubMed:9414270}. |
| Top Pathways | Arachidonic acid metabolism, Linoleic acid metabolism |
15-LO CRISPR/Cas9 KO Plasmid (h) - sc-401591 from Santa Cruz Biotechnology
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15-LO HDR Plasmid (h) - sc-401591-HDR from Santa Cruz Biotechnology
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15-LO Double Nickase Plasmid (h) - sc-401591-NIC from Santa Cruz Biotechnology
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15-LO Double Nickase Plasmid (h2) - sc-401591-NIC-2 from Santa Cruz Biotechnology
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15-LO CRISPR Activation Plasmid (h) - sc-401591-ACT from Santa Cruz Biotechnology
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15-LO CRISPR Activation Plasmid (h2) - sc-401591-ACT-2 from Santa Cruz Biotechnology
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15-LO Lentiviral Activation Particles (h) - sc-401591-LAC from Santa Cruz Biotechnology
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15-LO Lentiviral Activation Particles (h2) - sc-401591-LAC-2 from Santa Cruz Biotechnology
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15-LO siRNA (h) - sc-105003 from Santa Cruz Biotechnology
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15-LO shRNA Plasmid (h) - sc-105003-SH from Santa Cruz Biotechnology
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15-LO shRNA (h) Lentiviral Particles - sc-105003-V from Santa Cruz Biotechnology
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CompoZr® Knockout ZFN Kit - CKOZFN3079 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND3079 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR05692 from Sigma-Aldrich
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ALOX15 siRNA (Human) - CRH0170 from Cohesion Biosciences
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15-Lipoxygenase 1 RNAi - H00000246-R01 from Novus Biologicals
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ALOX15 - MBS8211424 from MyBioSource
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15-LO CRISPR/Cas9 KO Plasmid (h2) - sc-401591-KO-2 from Santa Cruz Biotechnology
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15-LO HDR Plasmid (h2) - sc-401591-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human ALOX15 (NM_001140.3) - SHH235366 from Creative biogene
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shRNA set against Human ALOX15(NM_001140.3) - SHG032029 from Creative biogene
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Human ALOX15 siRNA - orb260672 from Biorbyt
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ALOX15 shRNA Plasmid (Human) - CHH0170 from Cohesion Biosciences
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ALOX15 miRNA 3'UTR clone - MiUTR1H-00313 from Creative biogene
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