Apex1

Gene Symbol Apex1
Entrez Gene 11792
Alt Symbol APE, Apex, HAP1, Ref-1
Species Mouse
Gene Type protein-coding
Description apurinic/apyrimidinic endonuclease 1
Other Description AP endonuclease 1|APEN|APEX nuclease|DNA-(apurinic or apyrimidinic site) lyase|apurinic-apyrimidinic endonuclease 1|redox factor-1
Swissprots P28352
Accessions AAC13769 BAA07270 EDL20835 P28352 AK013296 BAB28774 AK178250 AK180111 AK186266 AK208670 AK212859 BC052401 AAH52401 D90374 BAA14382 XM_006518456 XP_006518519 NM_009687 NP_033817
Function Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3'
Subcellular Location Nucleus. Nucleus, nucleolus {ECO:0000250}. Nucleus speckle {ECO:0000255|PROSITE-ProRule:PRU00764}. Endoplasmic reticulum {ECO:0000250}. Cytoplasm. Note=Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (amino acid position 63-79). S-nitrosylation at Cys-92 and Cys-309 regulates its nuclear-cytosolic shuttling. Ubiquitinated form is localized predominantly in the cytoplasm. Detected in the cytoplasm of B-cells stimulated to switch (By similarity). {ECO:0000250}
Tissue Specificity Expressed in both resting and stimulated B cells stimulated to switch (at protein level).
Top Pathways Base excision repair

Anti-APE1 antibody - ab48840 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-APE1 antibody (ab48840) at 1.25 µg/ml + SP2/0 lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-HAP1 Antibody (aa562-573) - LS-C54959 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-APEX1 (AB2) antibody produced in rabbit - AV37013 from Sigma-Aldrich

Prices $267.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: SP2/0 (<B>Cat. No. AV37013</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 1.25 μg/mL in cell type SP2/0

Anti-APEX1 (AB3) antibody produced in rabbit - AV37014 from Sigma-Aldrich

Prices $267.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: SP2/0 (<B>Cat. No. AV37014</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 2.5 μg/mL in cell type SP2/0

APEX1 antibody - N-terminal region (ARP37013_T100) - ARP37013_T100 from Aviva Systems Biology

Prices $229.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

APEX1 antibody - N-terminal region (ARP37014_T100) - ARP37014_T100 from Aviva Systems Biology

Prices $229.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Hap1-mouse-Antibody-C-Term - AF2578a from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AF2578a (0.1 µg/ml) staining of Mouse Brain lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Hap1 antibody, C-term - GTX89095 from GeneTex

Prices $319.00
Sizes 100 μg
Host Goat
Clonality Polyclonal
Anti-Hap1 antibody, C-term used in Western blot. GTX89095

APEX1 antibody - 70R-31661 from Fitzgerald

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti -HAP1 (Huntingtin-associated Protein 1, HAP-1, Neuroan 1, HAP2, HLP1) - 150520 from USBiological Life Science

Host Goat
Clonality Polyclonal

APEX1 Antibody - 28-604 from ProSci

Prices $250.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

APEX1 Antibody - 28-605 from ProSci

Prices $250.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Antibody used in WB on Mouse SP2/0 at 2.5 ug/ml.

APEX1 Antibody - GWB-65015D from Genway Biotech

Prices $294.00
Sizes 100 µg
Clonality Polyclonal

APEX1 Antibody - GWB-457270 from Genway Biotech

Prices $294.00
Sizes 100 µg
Clonality Polyclonal

Polyclonal Antibody to Oxoguanine Glycosylase 1 (OGG1) - PAC704Mu01 from Cloud-clone

Host Rabbit
Clonality Polyclonal

Polyclonal Antibody to Oxoguanine Glycosylase 1 (OGG1) - PAC704Mu02 from Cloud-clone

Host Rabbit
Clonality Polyclonal

APEX1-antibody-N-terminal-region-AI11099 - AI11099 from Abgent, a WuXi AppTec company

Prices $232.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
WB Suggested Anti-APEX1 Antibody Titration: 1.25ug/ml ELISA Titer: 1:12500 Positive Control: SP2/0 cell lysate
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