| Gene Symbol | Apex1 |
|---|---|
| Entrez Gene | 11792 |
| Alt Symbol | APE, Apex, HAP1, Ref-1 |
| Species | Mouse |
| Gene Type | protein-coding |
| Description | apurinic/apyrimidinic endonuclease 1 |
| Other Description | AP endonuclease 1|APEN|APEX nuclease|DNA-(apurinic or apyrimidinic site) lyase|apurinic-apyrimidinic endonuclease 1|redox factor-1 |
| Swissprots | P28352 |
| Accessions | AAC13769 BAA07270 EDL20835 P28352 AK013296 BAB28774 AK178250 AK180111 AK186266 AK208670 AK212859 BC052401 AAH52401 D90374 BAA14382 XM_006518456 XP_006518519 NM_009687 NP_033817 |
| Function | Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' |
| Subcellular Location | Nucleus. Nucleus, nucleolus {ECO:0000250}. Nucleus speckle {ECO:0000255|PROSITE-ProRule:PRU00764}. Endoplasmic reticulum {ECO:0000250}. Cytoplasm. Note=Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (amino acid position 63-79). S-nitrosylation at Cys-92 and Cys-309 regulates its nuclear-cytosolic shuttling. Ubiquitinated form is localized predominantly in the cytoplasm. Detected in the cytoplasm of B-cells stimulated to switch (By similarity). {ECO:0000250} |
| Tissue Specificity | Expressed in both resting and stimulated B cells stimulated to switch (at protein level). |
| Top Pathways | Base excision repair |
Ref-1 CRISPR/Cas9 KO Plasmid (m) - sc-419148 from Santa Cruz Biotechnology
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Ref-1 HDR Plasmid (m) - sc-419148-HDR from Santa Cruz Biotechnology
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Ref-1 Double Nickase Plasmid (m) - sc-419148-NIC from Santa Cruz Biotechnology
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Ref-1 Double Nickase Plasmid (m2) - sc-419148-NIC-2 from Santa Cruz Biotechnology
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Ref-1 CRISPR Activation Plasmid (m) - sc-419148-ACT from Santa Cruz Biotechnology
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Ref-1 CRISPR Activation Plasmid (m2) - sc-419148-ACT-2 from Santa Cruz Biotechnology
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Ref-1 Lentiviral Activation Particles (m) - sc-419148-LAC from Santa Cruz Biotechnology
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Ref-1 Lentiviral Activation Particles (m2) - sc-419148-LAC-2 from Santa Cruz Biotechnology
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Ref-1 siRNA (m) - sc-36401 from Santa Cruz Biotechnology
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Ref-1 shRNA Plasmid (m) - sc-36401-SH from Santa Cruz Biotechnology
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Ref-1 shRNA (m) Lentiviral Particles - sc-36401-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EMU023881 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN26822 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND26822 from Sigma-Aldrich
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APEX1 siRNA (Mouse) - CRM0205 from Cohesion Biosciences
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APEX1 - MBS8225561 from MyBioSource
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shRNA set against Mouse APEX1 (NM_009687.2) - SHH238178 from Creative biogene
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Mouse APEX1 siRNA - orb279681 from Biorbyt
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APEX1 shRNA Plasmid (Mouse) - CHM0205 from Cohesion Biosciences
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