RWDD3

Gene Symbol RWDD3
Entrez Gene 25950
Alt Symbol RSUME
Species Human
Gene Type protein-coding
Description RWD domain containing 3
Other Description RWD domain-containing protein 3|RWD domain-containing sumoylation enhancer|RWD-containing sumoylation enhancer
Swissprots Q08AJ7 Q6FID3 Q9BX35 C9JI45 A6NP44 C9J9L7 A8K9F0 Q9Y3V2
Accessions EAW73019 EAW73020 EAW73021 EAW73022 EAW73023 Q9Y3V2 AA336253 AA336662 AA825527 AK292665 BAF85354 AK292699 BAF85388 AK296476 BAG59117 AL050062 CAB43254 AM392773 CAL37651 AM393257 CAL38135 BC010936 BC016307 BC065813 BC125142 AAI25143 BU858771 BX537497 BX648581 CA454169 CN306675 CR533493 CAG38524 DC409838 NM_001128142 NP_001121614 NM_001199682 NP_001186611 NM_001278247 NP_001265176 NM_001278248 NP_001265177 NM_015485 NP_056300 NR_103483 NR_103484
Function Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the: binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Isoform 1 and isoform 2 positively regulate the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Isoform 1 and isoform 2 negatively regulate the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Isoform 2 promotes the sumoylation and transcriptional activity of the glucocorticoid receptor NR3C1 and enhances the interaction of SUMO1 and NR3C1 with UBE2I/UBC9. Has no
Subcellular Location Nucleus {ECO:0000269|PubMed:17956732}. Cytoplasm {ECO:0000269|PubMed:17956732}. Note=Colocalizes with UBC9/UBE2I in nuclear spots.
Tissue Specificity Isoform 1 and isoform 2 are expressed in glioma tumors (at protein level). Expressed in a wide number of tissues with highest expression in cerebellum, pituitary, heart, kidney, liver, stomach, pancreas, prostate and spleen. Low levels in thalamus, spinal cord, esophagus, thymus, lung and peripheral blood leukocytes. A higher level expression seen in pituitary tumors as compared to the pituitary gland. {ECO:0000269|PubMed:17956732, ECO:0000269|PubMed:22009797, ECO:0000269|PubMed:23469069}.

RWDD3 (Y-17) - sc-244053 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-RWDD3 antibody - ab91555 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RWDD3 antibody (ab91555) at 1 µg/mlLane 1 : Rat kidney tissue lysateLane 2 : Rat kidney tissue lysate with blocking peptideLysates/proteins at 15 µg per lane.

Anti-RWDD3 antibody - ab128285 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-RWDD3 antibody (ab128285) at 1 µg/ml + 293T cell lysate at 10 µgdeveloped using the ECL technique

Anti-RWDD3 antibody - ab183875 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RWDD3 antibody (ab183875) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : Hela whole cell lysateLane 4 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-RWDD3 Antibody (C-Terminus) IHC-plus™ - LS-B5040 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-RWDD3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5040 concentration 5 ug/ml.

Anti-RWDD3 Antibody (N-Terminus) IHC-plus™ - LS-B5041 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-RWDD3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5041 concentration 10 ug/ml.

Anti-RWDD3 Antibody (aa174-204, AP) - LS-C240315 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RWDD3 Antibody (aa174-204, APC) - LS-C240316 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RWDD3 Antibody (aa174-204, Biotin) - LS-C240317 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RWDD3 Antibody (aa174-204, FITC) - LS-C240318 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RWDD3 Antibody (aa174-204, PE) - LS-C240321 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RWDD3 Antibody (aa174-204, Azide-free, HRP) - LS-C248065 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RWDD3 Antibody (aa174-204) - LS-C324869 from LifeSpan Bioscience

Prices $435.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RWDD3 Antibody (aa92-141) - LS-C151358 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RWDD3 antibody Western Blot of 293T.

RWDD3 Antibody - NBP1-72132 from Novus Biologicals

Prices $399.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: RWDD3 Antibody [NBP1-72132] - Staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

RWDD3 Antibody - NBP1-72133 from Novus Biologicals

Prices $399.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: RWDD3 Antibody [NBP1-72133] - Staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

RWDD3 Antibody - NBP1-76310 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: RWDD3 Antibody [NBP1-76310] - Rat kidney tissue lysate with RWDD3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

RWDD3 Antibody - NBP1-76309 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: RWDD3 Antibody [NBP1-76309] - Mouse kidney tissue lysate with RWDD3 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.

RWDD3 Antibody - H00025950-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: RWDD3 Antibody [H00025950-B01] - analysis of RWDD3 expression in transfected 293T cell line by RWDD3 MaxPab polyclonal antibody. Lane 1: RWDD3 transfected lysate (21.45 KDa). Lane 2: Non-transfected lysate.

Anti-RWDD3 antibody produced in rabbit - SAB3500105 from Sigma-Aldrich

Prices $316.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-RWDD3: <B>Cat. No. SAB3500105</B>: RWDD3 in mouse kidney tissue lysate with RWDD3 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.

Anti-RWDD3 antibody produced in rabbit - HPA046146 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-RWDD3: <B>Cat. No. HPA046146</B>: Immunohistochemical staining of human kidney shows distinct positivity in glomeruli.

Anti-RWDD3 antibody produced in rabbit - SAB3500104 from Sigma-Aldrich

Prices $316.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-RWDD3: <B>Cat. No. SAB3500104</B>: RWDD3 in rat kidney tissue lysate with RWDD3 antibody at1 μg/mL in (A) the absence and (B) the presence of blocking peptide.

RWDD3 antibody - middle region (ARP59371_P050) - ARP59371_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

RWDD3 antibody - orb75279 from Biorbyt

Prices $723.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Western blot analysis of rat kidney tissue lysate at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide using RWDD3 antibody

RWDD3 antibody - orb75280 from Biorbyt

Prices $723.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Western blot analysis of mouse kidney tissue lysate at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide using RWDD3 antibody
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