XRCC5
| Gene Symbol | XRCC5 |
|---|---|
| Entrez Gene | 7520 |
| Alt Symbol | KARP-1, KARP1, KU80, KUB2, Ku86, NFIV |
| Species | Human |
| Gene Type | protein-coding |
| Description | X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) |
| Other Description | 86 kDa subunit of Ku antigen|ATP-dependent DNA helicase 2 subunit 2|ATP-dependent DNA helicase II 80 kDa subunit|CTC box-binding factor 85 kDa subunit|CTC85|CTCBF|DNA repair protein XRCC5|Ku autoantigen, 80kDa|Ku86 autoantigen related protein 1|TLAA|X-ray repair cross-complementing protein 5|lupus Ku autoantigen protein p86|nuclear factor IV|thyroid-lupus autoantigen |
| Swissprots | Q9UCQ0 Q4VBQ5 A8K3X5 Q9UCQ1 P13010 Q53HH7 Q7M4N0 Q0Z7V0 |
| Accessions | AAC52087 AAD14875 AAY14659 AAY24231 ABG46942 CBX87168 EAW70562 EAW70563 P13010 AK026166 AK096408 AK222603 BAD96323 AK290740 BAF83429 BC019027 AAH19027 BC095442 AAH95442 BG717693 BP375944 BU184024 DQ892660 ABM83586 DQ895925 ABM86851 J04977 AAA59475 M30938 AAA36154 X57500 CAA40736 NM_021141 NP_066964 |
| Function | Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'- deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-el |
| Subcellular Location | Nucleus. Nucleus, nucleolus. Chromosome. |
| Top Pathways | Non-homologous end-joining |
Ku-86 CRISPR/Cas9 KO Plasmid (h) - sc-400549 from Santa Cruz Biotechnology
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Ku-86 HDR Plasmid (h) - sc-400549-HDR from Santa Cruz Biotechnology
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Ku-86 Double Nickase Plasmid (h) - sc-400549-NIC from Santa Cruz Biotechnology
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Ku-86 Double Nickase Plasmid (h2) - sc-400549-NIC-2 from Santa Cruz Biotechnology
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Ku-86 CRISPR Activation Plasmid (h) - sc-400549-ACT from Santa Cruz Biotechnology
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Ku-86 CRISPR Activation Plasmid (h2) - sc-400549-ACT-2 from Santa Cruz Biotechnology
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Ku-86 Lentiviral Activation Particles (h) - sc-400549-LAC from Santa Cruz Biotechnology
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Ku-86 Lentiviral Activation Particles (h2) - sc-400549-LAC-2 from Santa Cruz Biotechnology
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Ku-86 siRNA (h) - sc-29384 from Santa Cruz Biotechnology
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Ku-86 shRNA Plasmid (h) - sc-29384-SH from Santa Cruz Biotechnology
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Ku-86 shRNA (h) Lentiviral Particles - sc-29384-V from Santa Cruz Biotechnology
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Ku80/XRCC5 RNAi - H00007520-R02 from Novus Biologicals
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MISSION® esiRNA - EHU096711 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN1488 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1488 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1456 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR08219 from Sigma-Aldrich
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XRCC5 siRNA (Human) - CRH5140 from Cohesion Biosciences
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XRCC5 - MBS8236210 from MyBioSource
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shRNA set against Human XRCC5 (NM_021141.3) - SHH448128 from Creative biogene
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XRCC5 shRNA Plasmid (Human) - CHH5140 from Cohesion Biosciences
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XRCC5 miRNA 3'UTR clone - MiUTR3H-02422 from Creative biogene
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