AJUBA

Gene Symbol AJUBA
Entrez Gene 84962
Alt Symbol JUB
Species Human
Gene Type protein-coding
Description ajuba LIM protein
Other Description LIM domain-containing protein ajuba|jub, ajuba homolog
Swissprots D3DS37 Q96IF1 A8MX18
Accessions EAW66204 EAW66205 Q96IF1 AA909473 AK025567 AK096128 BAG53217 AW615335 AY169959 AAO37641 BC007580 AAH07580 BC016733 BC034968 AAH34968 BU543883 BX109281 CB105505 DQ893513 ABM84439 DQ896486 ABM87485 NM_001289097 NP_001276026 NM_032876 NP_116265 NM_198086 NP_932352
Function Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell- cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1- induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin tr
Subcellular Location Cytoplasm, cytoskeleton. Cell membrane. Cell junction. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, P-body. Note=Shuttles between the cytoplasm and the nucleus. Localizes on centrosomes during G2-M phase. Preferentially co- localizes with cadherin-adhesive complexes at sites of cell-cell contacts. Colocalizes with GFI1 in the nucleus.
Top Pathways Hippo signaling pathway

Ajuba Antibody - 4897 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from P19 cells (lane 1) and COS-7 cells transfected with Ajuba (lane 2), using Ajuba Antibody.

Ajuba (B-3) - sc-398008 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ajuba (B-3): sc-398008. Western blot analysis of Ajuba expression in non-transfected 293T: sc-117752 (A), human Ajuba transfected 293T: sc-170504 (B), Hep G2 (C) and Caco-2 (D) whole cell lysates.

Ajuba (E-3) - sc-374610 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ajuba (E-3): sc-374610. Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar and cytoplasmic localization.

Ajuba (F-9) - sc-514001 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ajuba (F-9): sc-514001. Western blot analysis of Ajuba expression in non-transfected 293T: sc-117752 (A), human Ajuba transfected 293T: sc-170504 (B), Hep G2 (C), PC-3 (D), Caco-2 (E) and HeLa (F) whole cell lysates.

Ajuba (F-11) - sc-514026 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ajuba (F-11): sc-514026. Western blot analysis of Ajuba expression in non-transfected: sc-117752 (A) and human Ajuba transfected: sc-170504 (B) 293T whole cell lysates.

Ajuba (C-13) - sc-46721 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Ajuba (C-13): sc-46721. Western blot analysis of Ajuba expression in non-transfected (A) and human Ajuba transfected (B) whole cell lysates.

Ajuba (H-155) - sc-67155 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Ajuba (H-155): sc-67155. Western blot analysis of Ajuba expression in non-transfected (A) and human Ajuba transfected (B) whole cell lysates.

Ajuba (K-13) - sc-46722 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Ajuba (K-13): sc-46722. Western blot analysis of Ajuba expression in non-transfected (A) and human Ajuba transfected (B) whole cell lysates.

Ajuba (M-13) - sc-46723 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Ajuba (M-13): sc-46723. Western blot analysis of Ajuba expression in non-transfected (A) and human Ajuba transfected (B) whole cell lysates.

Anti-Ajuba antibody - ab64451 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Ajuba antibody (ab64451) at 1 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 2 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)Lane 3 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate (ab3960)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Ajuba antibody - ab67666 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-Ajuba antibody (ab67666) at 1/500 dilutionLane 1 : Ajuba transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-Ajuba antibody [21G4] - ab151400 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 21G4
Anti-Ajuba antibody [21G4] (ab151400) at 1/100 dilution + HaCaT Human keratinocyte cells

Anti-JUB / Ajuba Antibody (aa224-239) - LS-C19041 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Ajuba Antibody - Western Blot. Western blot of Affinity Purified anti-Ajuba antibody shows detection of a 57-kD band consistent with the expected MW for Ajuba (arrowhead). Lanes correspond to 1) HeLa nuclear extract, and 2) HeLa, 3) A431, 4) Jurkat and 5) 293 whole cell lysates. Immunoprecipitation of Ajuba followed by western blotting may result in cleaner background staining. Approximately 5 ug of each preparation was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Ajuba antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time. Personal Communication. E. Pugacheva, Fox Chase Cancer Center, Philadelphia, PA.

Anti-JUB / Ajuba Antibody (aa224-239) - LS-C63850 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-JUB / Ajuba Antibody (aa1-121) - LS-C125020 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-JUB / Ajuba Antibody - LS-C126649 from LifeSpan Bioscience

Prices $680.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-JUB / Ajuba Antibody - LS-C126650 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-JUB / Ajuba Antibody - LS-C308568 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Ajuba Antibody [Unconjugated] - AF5594 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Western blot shows lysates of PC-3 human prostate cancer cell line, A549 human lung carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human Ajuba Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5594) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Ajuba at approximately 55 to 60 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Ajuba Antibody - NBP1-89570 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Ajuba Antibody [NBP1-89570] - Analysis in human cell line HepG2.

Ajuba Antibody - NB600-468 from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: Ajuba Antibody [NB600-468] - Analysis of a 57-kDa band consistent with the expected MW for Ajuba (arrowhead). Lanes correspond to 1) HeLa nuclear extract, and 2) HeLa, 3) A431, 4) Jurkat and 5) 293 whole cell lysates. Immunoprecipitation of Ajuba followed by western blotting may result in cleaner background staining. Approximately 5 ug of each preparation was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Ajuba antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time.

Ajuba Antibody - H00084962-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Ajuba Antibody - H00084962-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: Ajuba Antibody [H00084962-B01] - analysis of JUB expression in transfected 293T cell line by JUB MaxPab polyclonal antibody (B01). Lane 1: JUB transfected lysate (56.90 KDa). Lane 2: Non-transfected lysate.

Anti-JUB antibody produced in mouse - SAB1408142 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-JUB: <B>Cat. No. SAB1408142</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~59.2 kDa using JUB transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-AJUBA antibody produced in rabbit - HPA006171 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-JUB: <B>Cat. No. HPA006171</B>: Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
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