NLRP3

Gene Symbol NLRP3
Entrez Gene 114548
Alt Symbol AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, FCAS, FCAS1, FCU, MWS, NALP3, PYPAF1
Species Human
Gene Type protein-coding
Description NLR family, pyrin domain containing 3
Other Description NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3|NACHT, LRR and PYD containing protein 3|NACHT, LRR and PYD domains-containing protein 3|PYRIN-containing APAF1-like protein 1|caterpiller protein 1.1|cold autoinflammatory syndrome 1 protein|cryopyrin|nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3
Swissprots B2RC97 Q96P20 B7ZKT3 O75434 B7ZKS9 Q8TEU9 Q17RS2 B7ZKT2 Q6TG35 Q8TCW0 Q59H68 Q5JQS8 Q8WXH9 Q5JQS9
Accessions AAL12497 AAL12498 BAD51451 BAD51452 BAD51453 BAD51454 EAW77183 EAW77184 EAW77185 EAW77186 EAW77187 Q96P20 AB208891 BAD92128 AF054176 AAC39910 AF410477 AAL33908 AF418985 AAL14640 AF420469 AAL65136 AF427617 AAL33911 AF468522 AAL78632 AK027194 AK314998 BAG37494 AL601856 AY092033 AAM14669 AY422168 AAQ98889 BC117211 AAI17212 BC143359 AAI43360 BC143362 AAI43363 BC143363 AAI43364 DA244290 XM_005273036 XP_005273093 XM_005273037 XP_005273094 XM_011544048 XP_011542350 XM_011544049 XP_011542351 XM_011544050 XP_011542352 XM_011544051 XP_011542353 XM_011544052 XP_011542354 XM_011544053 XP_011542355 XM_011544054 XP_011542356 XM_011544055 XP_011542357 NM_001079821 NP_001073289 NM_001127461 NP_001120933 NM_001127462 NP_001120934 NM_001243133 NP_001230062 NM_004895 NP_004886 NM_183395 NP_899632
Function May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. {ECO:0000269|PubMed:11786556, ECO:0000269|PubMed:14662828}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:11786556, ECO:0000269|PubMed:14662828, ECO:0000269|PubMed:17164409}.
Tissue Specificity Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. {ECO:0000269|PubMed:11786556, ECO:0000269|PubMed:12032915, ECO:0000269|PubMed:17164409, ECO:0000269|PubMed:17907925}.
Top Pathways NOD-like receptor signaling pathway, Pertussis, Influenza A

NLRP3 (D2P5E) Rabbit mAb - 13158 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2P5E
Western blot analysis of extracts from THP-1 cells using NLRP3 (D2P5E) Rabbit mAb.

Cryopyrin (N-14) - sc-34411 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Cryopyrin (M-12) - sc-34410 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Cryopyrin (D-12) - sc-34408 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Cryopyrin (H-66) - sc-66846 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cryopyrin (H-66): sc-66846. Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of squamous epithelial cells.

Cryopyrin (6F12) - sc-134306 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Cryopyrin (6F12): sc-134306. Western blot analysis of Cryopyrin expression in human Cryopyrin transfected (A) and non-transfected (B) 293T whole cell lysates.

Anti-CIAS1 / NALP3 antibody - ab4207 from Abcam

Prices $390.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-CIAS1 / NALP3 antibody - ab91525 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CIAS1 / NALP3 antibody (ab91525) at 1 µg/mlLane 1 : K562 cell lysate without blocking peptideLane 2 : K562 cell lysate plus blocking peptideLysates/proteins at 15 µg per lane.

Anti-CIAS1 / NALP3 antibody - ab51952 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Immunohistochemistry of paraffin embedded Human tonsil labelling CIAS1/NALP3 with ab51952 at 2µg/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-CIAS1 / NALP3 antibody - ab91413 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-CIAS1 / NALP3 antibody (ab91413) at 1/100 dilution + MDA-MB231 cell line lysate at 35 µg

Anti-CIAS1 / NALP3 antibody - ab98151 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-CIAS1 / NALP3 antibody (ab98151) at 1/1500 dilution + Raji whole cell lysate at 30 µg

Anti-CIAS1 / NALP3 antibody [EPR4777] - ab109314 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4777
ab109314 staining CIAS1/NALP3 in Human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). The image show the expression of NLRP3 in alveolar macrophages.

Anti-CIAS1 / NALP3 antibody [EPR4777] (DyLight® 488) - ab139884 from Abcam

Prices $371.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4777
Flow cytometric analysis of HT1080 cells labelling CIAS1 / NALP3 (red) with ab139884 at 1/100 dilution, compared to a nonspecific control antibody (green).

Anti-CIAS1 / NALP3 antibody [EPR4777] (HRP) - ab196923 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4777
All lanes : Anti-CIAS1 / NALP3 antibody [EPR4777] (HRP) (ab196923) at 1/3000 dilutionLane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLane 2 : HT1080 (Human fibrosarcoma cell line) Whole Cell LysateLane 3 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-CIAS1 / NALP3 antibody [Nalpy3-a] - ab16097 from Abcam

Prices $379.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone Nalpy3-a
Overlay histogram showing Saos 2 cells stained with ab16097 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16097, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-CIAS1 / NALP3 antibody [nalpy3-b] - ab17267 from Abcam

Prices $400.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone nalpy3-b
All lanes : Anti-CIAS1 / NALP3 antibody [nalpy3-b] (ab17267) at 1/1000 dilutionLane 1 : Human THP-1 cell lysate - untreatedLane 2 : Human THP-1 cell lysate - treated with PMA + LPSLane 3 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated Horse anti-mouse IgG polyclonal at 1/2000 dilutionPerformed under reducing conditions.

Human CIAS1 / NALP3 full length protein - ab198069 from Abcam

Prices $837.00
Sizes 5 µg
4-20% SDS-PAGE analysis of 1 µg ab198069 with Coomassie staining.

Anti-NALP3 / NLRP3 Antibody (N-Terminus) - LS-C327991 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NALP3 / NLRP3 Antibody - LS-C341685 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-NALP3 / NLRP3 Antibody (aa158-207) IHC-plus™ - LS-B8262 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-NALP3 / NLRP3 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8262 dilution 5-10 ug/ml.

Anti-NALP3 / NLRP3 Antibody - LS-C334651 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of K562 cell line, using NALP3 antibody.

Anti-NALP3 / NLRP3 Antibody (aa4-223) - LS-C150009 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal
NALP3 in THP-1 Human Cell Line. NALP3 was detected in immersion fixed THP-1 human acute monocytic leukemia cells, unstimulated (lower panel) and stimulated (upper panel) with PMA and LPS, using Sheep Anti-Human NALP3 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Anti-NALP3 / NLRP3 Antibody - LS-C334192 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of K-562 cell line, using NLRP3 antibody.

Anti-NALP3 / NLRP3 Antibody (aa1-93, clone Cryo-1) - LS-C344930 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone Cryo-1
NLRP3 is detected in mouse macrophages using anti-NLRP3/NALP3 (mouse), mAb (Cryo-1) . Cell extracts from mouse macrophages, WT (lane 1) or NLRP3 KO (lane 2), were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-NLRP3/NALP3 (mouse), mAb (Cryo-1) (1 ug/ml). Proteins were visualized by a chemiluminescence detection system.

Anti-NALP3 / NLRP3 Antibody (aa1024-1036) IHC-plus™ - LS-B1766 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-NLRP3 antibody IHC of human peripheral blood leukocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1766 concentration 5 ug/ml.
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